Abstract:
:Autosomal dominant tubulointerstitial kidney disease associated to the MUC1 gene (ADTKD-MUC1; formerly MCKD1) belongs to a heterogeneous group of rare hereditary kidney diseases that is prototypically caused by frameshift mutations in the MUC1 repeat domain. The mutant MUC1 (insC) lacks the transmembrane domaine, exhibits aberant cellular topology, and hence might gain a function during the pathological process. To get insight into potential pathomechanisms we perform differential proteomics of extracellular vesicles shed by renal epithelia into the urine of patients. The study is based on three ADTKD patients and individual controls applying iTRAQ/LC-MS/MS. A total of 796 proteins were identified across all biological and technical replicates, and 298 proteins were quantified. A proportion of 47 proteins were fold-changed species. GO Term Enrichment analysis revealed proteins with significantly changed expression in ADTKD-associated extracellular vesicles as vesicular transport-associated proteins. Among these VTA1 is involved in the endosomal multivesicular body pathway associated with transport to lysosomes or export via exosomes. VTA1 is also claimed to play roles as a cofactor of the AAA ATPases VPS4A and VPS4B in the disassembly of ESCRT III. Protein interaction databases list VPS4B, CHMP2A, and IST1 as VTA1 binding partners. (Data are available via ProteomeXchange with identifier PXD008389.).
journal_name
Proteomicsjournal_title
Proteomicsauthors
Staubach S,Wenzel A,Beck BB,Rinschen MM,Müller S,Hanisch FGdoi
10.1002/pmic.201700456subject
Has Abstractpub_date
2018-04-01 00:00:00pages
e1700456issue
7eissn
1615-9853issn
1615-9861journal_volume
18pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200206)2:6<690::AID-PROT690>3.0.
更新日期:2002-06-01 00:00:00
abstract::Trophoblastic cell lines are widely used in in vitro studies of placental function as a surrogate for primary trophoblasts. To date, no reference proteomics dataset exists to directly compare the shared and unique characteristics of these cells. Here, we performed comparative proteomic profiling of the BeWo and HTR8/S...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700037
更新日期:2017-05-01 00:00:00
abstract::The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400072
更新日期:2014-10-01 00:00:00
abstract::In a previous report we demonstrated protein kinase C (PKC)-mediated phosphorylation of the ligand-binding domain (LBD) of orphan nuclear receptor TR2. In this report, we provide the evidence of PKC-mediated phosphorylation of the DNA-binding domain (DBD) of TR2. Two PKC target sites were predicted within the DBD, at ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500068
更新日期:2006-01-01 00:00:00
abstract::Proteases are critical in many physiological processes and the human genome encodes for 566 predicted proteolytic enzymes. Therefore, there is great interest in identifying and characterizing physiologic protease-substrate relationships. The coagulation cascade is a well-described network of serine proteases. However,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000674
更新日期:2011-06-01 00:00:00
abstract::Staphylococcus aureus is an opportunistic human pathogen, which can cause life-threatening disease. Proteome analyses of the bacterium can provide new insights into its pathophysiology and important facets of metabolic adaptation and, thus, aid the recognition of targets for intervention. However, the value of such pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500091
更新日期:2015-11-01 00:00:00
abstract::Although the importance of the extracellular signal-regulated kinase (ERK) pathway in regulating the transition from G1 to S has been extensively studied, its role during the G2/M transition is less well understood. Previous reports have shown that inhibition of the ERK pathway in mammalian cells delays entry as well ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600365
更新日期:2006-08-01 00:00:00
abstract::Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300465
更新日期:2003-10-01 00:00:00
abstract::Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900502
更新日期:2010-02-01 00:00:00
abstract::In recent years, standardization and quality control have become important key points in industry, e.g. in drug discovery and for developing medical products. Is quality control in academic Proteomics a minor problem nowadays, where standard data formats and public repositories for data sharing exist? In this article,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000441
更新日期:2011-03-01 00:00:00
abstract::Heat shock proteins (HSPs) are crucial for the maintenance of cell integrity during normal cellular growth as well as during pathophysiological conditions. While functioning mainly as molecular chaperones, HSPs also appear to be involved in diverse biological activities, such as apoptosis, carcinogenesis, and cytoprot...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400951
更新日期:2004-11-01 00:00:00
abstract::This study aimed at establishing a sensitive multiple reaction monitoring-mass spectrometry (MRM-MS) method for the quantification of the drug metabolizing cytochrome P450 (CYP)3A4 enzyme in human liver homogenates. Liver samples were subjected to trypsin digestion. MRM-MS analyses were performed using three transitio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500386
更新日期:2016-11-01 00:00:00
abstract::Unconjugated bilirubin (UCB) is a powerful antioxidant and a modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop a physiological jaundice, in case of severe hyperbilirubinemia UCB may become neurotoxic causing severe long-term neuronal damages, also kno...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900579
更新日期:2010-04-01 00:00:00
abstract::We bring a "spectrum" of classical data mining and statistical analysis methods to bear on discrimination of two groups of spectra from 24 diseased and 17 normal patients. Our primary goal is to accurately estimate the generalizability of this small dataset. After an aggressive preprocessing step that reduces consider...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300516
更新日期:2003-09-01 00:00:00
abstract::Colorectal cancer risk is increased when dietary folate intake is low, with or without a deficiency in methylenetetrahydrofolate reductase (MTHFR). We have observed that intestinal tumors are induced in mice fed low-folate diets, and that tumor incidence is increased when these mice also have MTHFR deficiency. This st...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400280
更新日期:2014-11-01 00:00:00
abstract::Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of these channels is tightly controlled by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signaling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP2 ), Ca2+ /calmodulin, and pho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700015
更新日期:2017-10-01 00:00:00
abstract::3Z-3-[(1H-pyrrol-2-yl)-methylidene]-1-(1-piperidinylmethyl)-1,3-2H-indol-2-one (Z24), a synthetic anti-angiogenic compound, inhibits the growth and metastasis of certain tumors. Previous works have shown that Z24 induces hepatotoxicity in rodents. We examined the hepatotoxic mechanism of Z24 at the protein level and l...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900699
更新日期:2010-08-01 00:00:00
abstract::Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200356
更新日期:2013-03-01 00:00:00
abstract::Oligomeric ubiquitin structures (i.e. ubiquitin "chains") may be formed through any of seven different lysine residues in the polypeptide, or via the amine group of Met 1. Different types of ubiquitin chains can confer very different biological outcomes to a protein substrate, yet the structural characteristics of E2s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500058
更新日期:2015-09-01 00:00:00
abstract::Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000382
更新日期:2011-02-01 00:00:00
abstract::Myogenesis is a complex process of which the underlying mechanisms are conserved between species, including birds and mammals. Despite a good understanding of the stages of myogenesis, many of the mechanisms involved in the regulation of proliferation of the successive myoblast generations, the cellular transitions ce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701101
更新日期:2008-10-01 00:00:00
abstract::We have developed a proteome database (DB), BiomarkerDigger (http://biomarkerdigger.org) that automates data analysis, searching, and metadata-gathering function. The metadata-gathering function searches proteome DBs for protein-protein interaction, Gene Ontology, protein domain, Online Mendelian Inheritance in Man, a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800593
更新日期:2009-07-01 00:00:00
abstract::Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A2/B1, and A3) play an important role in proliferation, although the functional overlap among members of hnRNPs A/B remains largely unknown. In this study, we have employed RNAi knockdown and proteomic approaches to investigate the biological functions of h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300549
更新日期:2014-06-01 00:00:00
abstract::Warm ischemia reperfusion (IR) injury of the liver is associated with changes in the expression and/or post-translational modification of numerous proteins. Only a few of these have been identified. We used 2-D DIGE to identify cytosolic proteins altered in the early stage of IR in an established rat model of segmenta...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100053
更新日期:2011-11-01 00:00:00
abstract::Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700139
更新日期:2007-08-01 00:00:00
abstract::The hypothalamus is the central regulatory unit that balances a number of body functions including metabolic rate, hunger, and satiety signals. Hypothalamic neurons monitor and respond to alterations of circulating nutrients and hormones that reflect the peripheral energy status. These extracellular signals are integr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700248
更新日期:2007-10-01 00:00:00
abstract::Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500129
更新日期:2016-02-01 00:00:00
abstract::A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500721
更新日期:2006-05-01 00:00:00
abstract::CD28 delivers a co-stimulatory signal for T cell antigen receptor induced activation of T cells through a mechanism which remains mostly elusive to date. In order to try and gain insight into CD28 function, we therefore applied state-of-the-art mass spectrometric protein identification technology to the analysis of CD...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200101)1:1<70::AID-PROT70>3.0.CO
更新日期:2001-01-01 00:00:00
abstract::Lipid particles (LP) of all types of cells are a depot of neutral lipids. The present investigation deals with the isolation of LP from the yeast Yarrowia lipolytica and the characterization of their lipid and protein composition. Properties of LP varied depending on the carbon source. LP from glucose-grown cells reve...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500339
更新日期:2006-03-01 00:00:00