Urinary Proteomics Associates with COVID-19 Severity: Pilot Proof-of-Principle Data and Design of a Multicentric Diagnostic Study.

abstract：:Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking t...

journal_title：Proteomics

authors： Anand S,Foot N,Ang CS,Gembus KM,Keerthikumar S,Adda CG,Mathivanan S,Kumar S

更新日期：2018-09-01 00:00:00

abstract：:Diurnal physiological acclimation regulated by a circadian system is an advantage for plant fitness. The circadian system is composed of a signal input, the clock and output pathways. Understanding the regulation mechanism of the output pathways remains a major challenge. Diurnal proteomic change reflects the state of...

journal_title：Proteomics

authors： Wang Z,Wang T

更新日期：2011-01-01 00:00:00

abstract：:The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...

journal_title：Proteomics

authors： Kasyapa C,Gu TL,Nagarajan L,Polakiewicz R,Cowell JK

更新日期：2009-08-01 00:00:00

abstract：:CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...

journal_title：Proteomics

authors： Kim MS,Zhong J,Kandasamy K,Delanghe B,Pandey A

更新日期：2011-06-01 00:00:00

abstract：:A classical proteomic analysis was used to establish a reference map of proteins associated with healthy human erythrocyte ghosts. Following osmotic lysis and differential centrifugation, ghost proteins were separated by either one-dimensional gel electrophoresis (1-DE) or two-dimensional gel electrophoresis (2-DE). S...

journal_title：Proteomics

authors： Low TY,Seow TK,Chung MC

更新日期：2002-09-01 00:00:00

abstract：:A curated set of 81 proven and 44 predicted lipoproteins of Escherichia coli K-12 was defined with the combined use of a literature survey, a variety of predictive tools and human expertise. The well-documented Gram-negative proteome of E. coli K-12 was chosen to assess how the different approaches complement each oth...

journal_title：Proteomics

authors： Gonnet P,Rudd KE,Lisacek F

更新日期：2004-06-01 00:00:00

abstract：:The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...

journal_title：Proteomics

authors： Schaumburg J,Diekmann O,Hagendorff P,Bergmann S,Rohde M,Hammerschmidt S,Jänsch L,Wehland J,Kärst U

更新日期：2004-10-01 00:00:00

abstract：:A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...

journal_title：Proteomics

authors： Huelseweh B,Ehricht R,Marschall HJ

更新日期：2006-05-01 00:00:00

abstract：:In the era of quantitative proteomics, where mass spectrometry plays a pivotal role, in particular associated with the use of data-independent acquisition, it is time to perform an overview of this growing field with special focus on one of the most promising approaches: SWATH-MS, and to present future perspectives fo...

journal_title：Proteomics

authors： Anjo SI,Santa C,Manadas B

更新日期：2017-02-01 00:00:00

abstract：:DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...

journal_title：Proteomics

authors： Timms JF,Cramer R

更新日期：2008-12-01 00:00:00

abstract：:A multiplexed fluorescence immunoassay using a novel planar waveguide technology-based microarray system, ZeptoMARK (Zeptosens), was developed to detect simultaneously urokinase-type plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1), and vascular endothelial growth factor (VEGF) in extracts of bre...

journal_title：Proteomics

authors： Weissenstein U,Schneider MJ,Pawlak M,Cicenas J,Eppenberger-Castori S,Oroszlan P,Ehret S,Geurts-Moespot A,Sweep FC,Eppenberger U

更新日期：2006-03-01 00:00:00

abstract：:Ovarian cancer is one of the leading causes of mortality by gynecological cancer. Despite good response to surgery and initial chemotherapy, essentially based on cisplatin (cis-diamino-dichloro-platinum(II) (CDDP)) compounds, frequent recurrences with chemoresistance acquisition are responsible for poor prognosis. Sev...

journal_title：Proteomics

authors： Le Moguen K,Lincet H,Marcelo P,Lemoisson E,Heutte N,Duval M,Poulain L,Vinh J,Gauduchon P,Baudin B

更新日期：2007-11-01 00:00:00

abstract：:We previously reported that NSC606985, a new camptothecin analog, induces apoptosis of acute myeloid leukemic cells, which is triggered by proteolytic activation of protein kinase C delta (PKC delta). Here, we performed quantitative proteomic analysis of NSC606985-treated and untreated leukemic U937 cells with two-dim...

journal_title：Proteomics

authors： Zheng Y,Wang LS,Xia L,Han YH,Liao SH,Wang XL,Cheng JK,Chen GQ

更新日期：2009-04-01 00:00:00

abstract：:Sirtuins are a family of NAD(+)-dependent protein deacetylases that regulate cellular functions through deacetylation of a wide range of protein targets. Overexpression of Sir2, the first gene discovered in this family, is able to extend the life span in various organisms. The anti-aging effects of human homologues of...

journal_title：Proteomics

authors： Law IK,Liu L,Xu A,Lam KS,Vanhoutte PM,Che CM,Leung PT,Wang Y

更新日期：2009-05-01 00:00:00

abstract：:The main goal of this study was to analyze, using proteomic techniques, changes in protein expression of acute myeloid leukemia (AML) cells that could give insights into a better early prognosis for tumor pathophysiology. Proteomic analysis of different subtypes of AML cells was carried out using 2-DE and MALDI-TOF PM...

journal_title：Proteomics

authors： López-Pedrera C,Villalba JM,Siendones E,Barbarroja N,Gómez-Díaz C,Rodríguez-Ariza A,Buendía P,Torres A,Velasco F

更新日期：2006-04-01 00:00:00

abstract：:Ischemia/reperfusion (I/R) injury is a major consequence of a cardiovascular intervention. The study of changes of the left and right ventricle proteomes from hearts subjected to I/R may be a key to revealing the pathological mechanisms underlying I/R-induced heart contractile dysfunction. Isolated rat hearts were per...

journal_title：Proteomics

authors： Cadete VJ,Lin HB,Sawicka J,Wozniak M,Sawicki G

更新日期：2012-08-01 00:00:00

abstract：:Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due ...

journal_title：Proteomics

authors： Yuan C,Jiao L,Yang L,Ying W,Hu Z,Liu J,Cui F,Li L,Qian L,Teng Y,Hang H,Qian X,Yang X

更新日期：2008-06-01 00:00:00

abstract：:Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...

journal_title：Proteomics

authors： Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

更新日期：2011-04-01 00:00:00

abstract：:In this study, poly(glycidyl methacrylate-ethyleneglycol dimethacrylate) monolith functionalized with cobalt phthalocyanine tetracarboxylic acid is prepared. The polymer monolithic material is used for glycopeptides enrichment coupled with MALDI-TOF MS. By taking advantage of cobalt phthalocyanine including hydrogen b...

journal_title：Proteomics

authors： Zhang W,Song N,Zheng H,Feng W,Jia Q

更新日期：2018-10-01 00:00:00

abstract：:This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. T...

journal_title：Proteomics

authors： Heo SH,Choi JH,Kim YM,Jung CW,Lee J,Jin HY,Kim GH,Lee BH,Shin CH,Yoo HW

更新日期：2013-04-01 00:00:00

abstract：:In mass spectrometry-based proteomics, protein identification results usually consist of peptide sequences and database-dependent accession identifiers of the matching proteins. Often certain annotations are only available in particular databases that in turn must be queried by a certain identifier. In order to simpli...

journal_title：Proteomics

authors： Boehm AM,Sickmann A

更新日期：2006-08-01 00:00:00

abstract：:Exercise training has been recommended as a nonpharmacological strategy for the prevention and attenuation of skeletal muscle atrophy in distinct pathophysiological conditions. Despite the well-established phenotypic alterations, the molecular mechanisms underlying exercise-induced skeletal muscle remodeling are poorl...

journal_title：Proteomics

authors： Padrão AI,Ferreira R,Amado F,Vitorino R,Duarte JA

更新日期：2016-03-01 00:00:00

abstract：:The phosphorylation of heat shock protein 27 (HSP27) occurs differently in human renal cell carcinoma (RCC) compared to homologous normal kidney tissue. Two-dimensional electrophoresis was used to separate and visualize HSP27, via immunostaining with anti-HSP27 antibody, in tumor and normal renal samples, obtained aft...

journal_title：Proteomics

authors： Tremolada L,Magni F,Valsecchi C,Sarto C,Mocarelli P,Perego R,Cordani N,Favini P,Galli Kienle M,Sanchez JC,Hochstrasser DF,Corthals GL

更新日期：2005-02-01 00:00:00

abstract：:The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...

journal_title：Proteomics

authors： Marengo E,Robotti E,Antonucci F,Cecconi D,Campostrini N,Righetti PG

更新日期：2005-02-01 00:00:00

abstract：:The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A l...

journal_title：Proteomics

authors： Korf U,Derdak S,Tresch A,Henjes F,Schumacher S,Schmidt C,Hahn B,Lehmann WD,Poustka A,Beissbarth T,Klingmüller U

更新日期：2008-11-01 00:00:00

abstract：:Proteolytic modification of components of the extracellular milieu by metalloproteinases plays important roles in the regulation of multiple cellular and physiological processes and pathological conditions. ADAMTS1 is a secreted enzyme of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) fami...

journal_title：Proteomics

authors： Canals F,Colomé N,Ferrer C,Plaza-Calonge Mdel C,Rodríguez-Manzaneque JC

更新日期：2006-04-01 00:00:00

abstract：:Advanced glycation endproduct (AGE) formation is an important mechanism for protein deterioration during diabetic complications and ageing. The effects on AGE formation following dihydroxyacetone (DHA) stress were studied in two model organisms, the yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegan...

journal_title：Proteomics

authors： Molin M,Pilon M,Blomberg A

更新日期：2007-10-01 00:00:00

abstract：:Biotherapeutics, molecules produced from biological systems, require rigorous purification steps to remove impurities including host cell proteins (HCPs). Regulatory guidelines require manufacturers to monitor process-related impurities along the purification workflow. Mass spectrometry (MS) has recently been consider...

journal_title：Proteomics

authors： Trauchessec M,Hesse AM,Kraut A,Berard Y,Herment L,Fortin T,Bruley C,Ferro M,Manin C

更新日期：2021-01-18 00:00:00

abstract：:A characteristic of two-dimensional proteomics gels is a general bimodal distribution of isoelectric (pI) values. Discussion of this feature has focussed on the balance of acidic and basic ionisable residues, and potential relationships between pI distributions and organism classification or protein subcellular locati...

journal_title：Proteomics

authors： Chan P,Lovrić J,Warwicker J

更新日期：2006-06-01 00:00:00

abstract：:Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...

journal_title：Proteomics

authors： Lee AY,Goo Park S,Kho CW,Young Park S,Cho S,Lee SC,Lee DH,Myung PK,Park BC

更新日期：2004-11-01 00:00:00