Abstract:
:A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich-enzyme-linked immunosorbent assay and signal amplification by streptavidin-poly-horseradish peroxidase. Specific immunoassays for Staphylococcus enterotoxin B, ricin, Venezuelan equine encephalitis virus, St. Louis encephalitis virus, West Nile virus, Yellow fever virus, Orthopox virus species, Francisella tularensis, Yersinia pestis, Brucella melitensis, Burkholderia mallei and Escherichia coli EHEC O157:H7 were developed and optimized. All assays could be completed within 1 to 1 1/2 h and detection levels were demonstrated to be as low as in well established ELISAs. Most interesting, as a result of careful antibody screening and testing, it is currently possible to analyse at least five of the "dirty dozen" agents on one single protein chip in parallel.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Huelseweh B,Ehricht R,Marschall HJdoi
10.1002/pmic.200500721subject
Has Abstractpub_date
2006-05-01 00:00:00pages
2972-81issue
10eissn
1615-9853issn
1615-9861journal_volume
6pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Proteases are critical in many physiological processes and the human genome encodes for 566 predicted proteolytic enzymes. Therefore, there is great interest in identifying and characterizing physiologic protease-substrate relationships. The coagulation cascade is a well-described network of serine proteases. However,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000674
更新日期:2011-06-01 00:00:00
abstract::Here, we describe the Interactorium, a tool in which a Virtual Cell is used as the context for the seamless visualisation of the yeast protein interaction network, protein complexes and protein 3-D structures. The tool has been designed to display very complex networks of up to 40 000 proteins or 6000 multiprotein com...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900260
更新日期:2009-12-01 00:00:00
abstract::Proteins determine the quality of barley in malting and brewing end-uses. In this regard, water-soluble barley proteins play a major role in the formation, stability, and texture of head foams. Our objective was to survey the barley seed proteins that could be involved in the foaming properties of beer. Therefore, two...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401153
更新日期:2005-07-01 00:00:00
abstract::Despite affecting up to 70% of HIV-positive patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600236
更新日期:2017-03-01 00:00:00
abstract::A novel proteolysis approach was developed by using infrared (IR) radiation and trypsin-immobilized silica microspheres. Protein solutions containing trypsin-immobilized microspheres in sealed transparent Eppendorf tubes were allowed to digest under an IR lamp at 37 degrees C. The feasibility and performance of the pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800659
更新日期:2009-02-01 00:00:00
abstract::Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrP(TSE)) of the host-encoded cellular prion protein (PrP(C)). PrP(TSE) has a central role in the pathogenesis of the disease but other factors are likely involved ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900085
更新日期:2009-08-01 00:00:00
abstract::To date, proteomics approaches have aimed to either identify novel proteins or change in protein expression/modification in various organisms under normal or disease conditions. One major aspect of functional proteomics is to identify protein biological properties in a given context, however, forward proteomics approa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600211
更新日期:2006-10-01 00:00:00
abstract::Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200070
更新日期:2012-08-01 00:00:00
abstract::We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500511
更新日期:2006-03-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs. A large-scale outbreak of PED occurred in China in 2010, with PEDV emerging in the United States in 2013 and spreading rapidly, posing significant economic and p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400458
更新日期:2015-06-01 00:00:00
abstract::Lipid particles (LP) of all types of cells are a depot of neutral lipids. The present investigation deals with the isolation of LP from the yeast Yarrowia lipolytica and the characterization of their lipid and protein composition. Properties of LP varied depending on the carbon source. LP from glucose-grown cells reve...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500339
更新日期:2006-03-01 00:00:00
abstract::Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenes...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000460
更新日期:2011-06-01 00:00:00
abstract::The Annual 2014 Spring Workshop of the Proteomics Standards Initiative (PSI) of the Human Proteome Organization (HUPO) was held this year jointly with the metabolomics COordination of Standards in MetabOlomicS (COSMOS) group. The range of existing MS standards (mzML, mzIdentML, mzQuantML, mzTab, TraML) was reviewed an...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.201470164
更新日期:2014-11-01 00:00:00
abstract::Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. I...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300736
更新日期:2004-05-01 00:00:00
abstract::Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000382
更新日期:2011-02-01 00:00:00
abstract::Trophoblastic cell lines are widely used in in vitro studies of placental function as a surrogate for primary trophoblasts. To date, no reference proteomics dataset exists to directly compare the shared and unique characteristics of these cells. Here, we performed comparative proteomic profiling of the BeWo and HTR8/S...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700037
更新日期:2017-05-01 00:00:00
abstract::Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600693
更新日期:2007-03-01 00:00:00
abstract::With recent publications of several large-scale protein-protein interaction (PPI) studies, the realization of the full yeast interaction network is getting closer. Here, we have analysed several yeast protein interaction datasets to understand their strengths and weaknesses. In particular, we investigate the effect of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800150
更新日期:2008-11-01 00:00:00
abstract::Colorectal cancer risk is increased when dietary folate intake is low, with or without a deficiency in methylenetetrahydrofolate reductase (MTHFR). We have observed that intestinal tumors are induced in mice fed low-folate diets, and that tumor incidence is increased when these mice also have MTHFR deficiency. This st...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400280
更新日期:2014-11-01 00:00:00
abstract::This review provides a brief overview of the development of data-independent acquisition (DIA) mass spectrometry-based proteomics and selected DIA data analysis tools. Various DIA acquisition schemes for proteomics are summarized first including Shotgun-CID, DIA, MSE , PAcIFIC, AIF, SWATH, MSX, SONAR, WiSIM, BoxCar, S...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900276
更新日期:2020-09-01 00:00:00
abstract::Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected by Citrus exocortis viroid (CEVd). Among the differentially expressed proteins detected, 45 were su...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200286
更新日期:2013-03-01 00:00:00
abstract::The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400928
更新日期:2004-10-01 00:00:00
abstract::Normal sera contain a large number of naturally occurring autoantibodies which can mask important disease-associated ones. Western blotting has evolved as the most important tool to demonstrate autoantibodies in autoimmune diseases, because of its ability to simultaneous screening for a wide spectrum of different anti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300375
更新日期:2003-06-01 00:00:00
abstract::Serine/threonine protein phosphatase 1 (PP1) regulates multiple cellular processes. Protein phosphorylation-dephosphorylation is largely altered during ischemia and subsequent reperfusion. The brain is particularly vulnerable to stress resulting from ischemia-reperfusion (IR), however, the acquisition of ischemic tole...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700214
更新日期:2007-09-01 00:00:00
abstract::Biological membranes are compartmentalized into microdomains that exhibit particular lipid and protein compositions. Membrane microdomains, such as tetraspanin-enriched microdomains and lipid rafts, have been suggested to play a role in a variety of physiological and pathological processes. Therefore, the characteriza...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600282
更新日期:2006-12-01 00:00:00
abstract::Most proteins execute their functions through interacting with other proteins. Thus, understanding protein-protein interactions (PPIs) is essential to decipher biological functions in a living cell. To predict large-scale PPIs, effective and efficient computational approaches are desirable to integrate heterogeneous d...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200326
更新日期:2013-01-01 00:00:00
abstract::Separation and identification of hydrophobic membrane proteins is a major challenge in proteomics. Identification of such sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins by peptide mass fingerprinting (PMF) via matrix-assisted laser desorption/ionization-time of flight (MALDI-TO...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400851
更新日期:2004-12-01 00:00:00
abstract::Arabidopsis thaliana cell suspension cultures have been used to investigate the effects of salinity and hyperosmotic stress on plant cellular proteins. We show that 200 mM NaCl and 400 mM sorbitol treatments induce extracellular medium acidification in Arabidopsis cell cultures, a typical response of plant cells to sa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401282
更新日期:2005-11-01 00:00:00
abstract::Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400241
更新日期:2015-04-01 00:00:00
abstract::Niemann-Pick disease, type C1 (NPC1) is a fatal, autosomal recessive, neurodegenerative disorder caused by mutations in the NPC1 gene. As a result of the genetic defect, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal/lysosomal system causing both visceral and neurological def...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800285
更新日期:2019-09-01 00:00:00