Abstract:
:Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. In contrast, we use a second chip surface for the local application of detection antibodies, thereby efficiently eliminating antibody cross-reactions. Here, we illustrate the potential advantages of using single-chain Fv fragments rather than mAbs as capture and detection molecules with this double chip technology.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Gilbert I,Schiffmann S,Rubenwolf S,Jensen K,Mai T,Albrecht C,Lankenau A,Beste G,Blank K,Gaub HE,Clausen-Schaumann Hdoi
10.1002/pmic.200300736subject
Has Abstractpub_date
2004-05-01 00:00:00pages
1417-20issue
5eissn
1615-9853issn
1615-9861journal_volume
4pub_type
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