Abstract:
:The quality of samples and of pre-analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two-dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered in order to obtain high quality and reproducibility of spots. The mechanical dissection and cell separation by magnetic beads coated with anti-Ber and EP4 antibodies to minimize the contamination of nonepithelial cells are described.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Sarto C,Valsecchi C,Mocarelli Pdoi
10.1002/1615-9861(200211)2:11<1627::AID-PROT1627>3subject
Has Abstractpub_date
2002-11-01 00:00:00pages
1627-9issue
11eissn
1615-9853issn
1615-9861journal_volume
2pub_type
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