Abstract:
:Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS analysis often yields a large number of hits of borderline statistical confidence. To simplify their validation, we propose to use rapid de novo interpretation of all acquired MS/MS spectra and, with the help of a simple software tool, display the candidate sequences together with each database search hit. We demonstrate that comparing hit database sequences and independent de novo interpretations of the same MS/MS spectra assists in rapid examination of ambiguous matches.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Thomas H,Shevchenko Adoi
10.1002/pmic.200800250subject
Has Abstractpub_date
2008-10-01 00:00:00pages
4173-7issue
20eissn
1615-9853issn
1615-9861journal_volume
8pub_type
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