Abstract:
:A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differentiated from their target analytes by LC-MS due to individual retention times and/or by MS/MS due to specific fragment ions. Both quantification methods are demonstrated using peptide mixtures of low and high complexity.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Winter D,Seidler J,Kugelstadt D,Derrer B,Kappes B,Lehmann WDdoi
10.1002/pmic.200900695subject
Has Abstractpub_date
2010-04-01 00:00:00pages
1510-4issue
7eissn
1615-9853issn
1615-9861journal_volume
10pub_type
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