Protein composition of oil bodies from mature Brassica napus seeds.

abstract：:Antibody-based proteomics applied to tissue microarray (TMA) technology provides a very efficient means of visualizing and locating antigen expression in large collections of normal and pathological tissue samples. To characterize antigen expression on TMAs, the use of image analysis methods avoids the effects of huma...

journal_title：Proteomics

authors： Decaestecker C,Lopez XM,D'Haene N,Roland I,Guendouz S,Duponchelle C,Berton A,Debeir O,Salmon I

更新日期：2009-10-01 00:00:00

abstract：:Current treatments for advanced prostate cancer focus on inhibition of the androgen receptor (AR) by androgen deprivation therapy (ADT). However, complex interactions mediated by tumor suppressors, oncogenes, aberrations of AR expression, or de novo androgen production have been shown to induce the adaptive response o...

journal_title：Proteomics

authors： Soekmadji C,Rockstroh A,Ramm GA,Nelson CC,Russell PJ

更新日期：2017-12-01 00:00:00

abstract：:MDM2 is a multidomain protein that functions as an E3 ubiquitin ligase, transcription repressor, mRNA-binding protein, translation factor, and molecular chaperone. The small molecule Nutlin-3 has been engineered to bind to the N-terminal hydrophobic pocket domain of MDM2. This binding of Nutlin-3 has two consequences:...

journal_title：Proteomics

authors： Hernychova L,Man P,Verma C,Nicholson J,Sharma CA,Ruckova E,Teo JY,Ball K,Vojtesek B,Hupp TR

更新日期：2013-08-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:The gut microbiome has been shown to play a significant role in human healthy and diseased states. The dynamic signaling that occurs between the host and microbiome is critical for the maintenance of host homeostasis. Analyzing the human microbiome with metaproteomics, metabolomics, and integrative multi-omics analyse...

journal_title：Proteomics

authors： Peters DL,Wang W,Zhang X,Ning Z,Mayne J,Figeys D

更新日期：2019-08-01 00:00:00

abstract：:Gene expression analyses of embryonic stem cells (ESCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal and pluripotency. We employed a 2-DE-based proteomics approach to analyze human ESC line, Royan H5, in undifferentiated cells and differ...

journal_title：Proteomics

authors： Fathi A,Pakzad M,Taei A,Brink TC,Pirhaji L,Ruiz G,Sharif Tabe Bordbar M,Gourabi H,Adjaye J,Baharvand H,Salekdeh GH

更新日期：2009-11-01 00:00:00

abstract：:FtsH is the only membrane-bound and essential protease in Escherichia coli. It is responsible for the degradation of regulatory proteins and enzymes such as the heat-shock sigma factor RpoH or LpxC, the key enzyme of lipopolysaccharide biosynthesis. To find new FtsH targets, we trapped substrates in E. coli cells from...

journal_title：Proteomics

authors： Arends J,Thomanek N,Kuhlmann K,Marcus K,Narberhaus F

更新日期：2016-12-01 00:00:00

abstract：:The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...

journal_title：Proteomics

authors： Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

更新日期：2019-11-01 00:00:00

abstract：:The discovery of PTMs in proteins by MS requires nearly complete sequence coverage of the detected proteolytic peptides. Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and/or signal suppression in complex samples. When several lysine resid...

journal_title：Proteomics

authors： Markoutsa S,Bahr U,Papasotiriou DG,Häfner AK,Karas M,Sorg BL

更新日期：2014-03-01 00:00:00

abstract：:The rice heterotrimeric G-protein complex, a guanine-nucleotide-dependent on-off switch, mediates vital cellular processes and responses to biotic and abiotic stress. Exchange of bound GDP (resting state) for GTP (active state) is spontaneous in plants including rice and thus there is no need for promoting guanine nuc...

journal_title：Proteomics

authors： Biswal AK,McConnell EW,Werth EG,Lo SF,Yu SM,Hicks LM,Jones AM

更新日期：2019-05-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:Unconjugated bilirubin (UCB) is a powerful antioxidant and a modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop a physiological jaundice, in case of severe hyperbilirubinemia UCB may become neurotoxic causing severe long-term neuronal damages, also kno...

journal_title：Proteomics

authors： Deganuto M,Cesaratto L,Bellarosa C,Calligaris R,Vilotti S,Renzone G,Foti R,Scaloni A,Gustincich S,Quadrifoglio F,Tiribelli C,Tell G

更新日期：2010-04-01 00:00:00

abstract：:By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...

journal_title：Proteomics

authors： Medjahed D,Luke BT,Tontesh TS,Smythers GW,Munroe DJ,Lemkin PF

更新日期：2003-08-01 00:00:00

abstract：:Myostatin plays a major role in muscle growth and development and animals with disruption of this gene display marked increases in muscle mass. Little is known about muscle physiological adaptations in relation to this muscle hypertrophy. To provide a more comprehensive view, we analyzed bovine muscles from control, h...

journal_title：Proteomics

authors： Bouley J,Meunier B,Chambon C,De Smet S,Hocquette JF,Picard B

更新日期：2005-02-01 00:00:00

abstract：:Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...

journal_title：Proteomics

authors： Echan LA,Tang HY,Ali-Khan N,Lee K,Speicher DW

更新日期：2005-08-01 00:00:00

abstract：:Most proteins in all organisms undergo crucial N-terminal modifications involving N-terminal methionine excision, N-alpha-acetylation or N-myristoylation (N-Myr), or S-palmitoylation. We investigated the occurrence of these poorly annotated but essential modifications in proteomes, focusing on eukaryotes. Experimental...

journal_title：Proteomics

authors： Martinez A,Traverso JA,Valot B,Ferro M,Espagne C,Ephritikhine G,Zivy M,Giglione C,Meinnel T

更新日期：2008-07-01 00:00:00

abstract：:A proteomic analysis of a wild-type and of a phoB mutant showed that Vibrio cholerae expresses genes of two major regulons in response to phosphate starvation. The Pho regulon, expressed by the wild-type, allowed the cells to adapt to the new environment. Induction of the general stress regulon was mainly observed in ...

journal_title：Proteomics

authors： von Krüger WM,Lery LM,Soares MR,de Neves-Manta FS,Batista e Silva CM,Neves-Ferreira AG,Perales J,Bisch PM

更新日期：2006-03-01 00:00:00

abstract：:GlycoMod (http://www.expasy.ch/tools/glycomod/) is a software tool designed to find all possible compositions of a glycan structure from its experimentally determined mass. The program can be used to predict the composition of any glycoprotein-derived oligosaccharide comprised of either underivatised, methylated or ac...

journal_title：Proteomics

authors： Cooper CA,Gasteiger E,Packer NH

更新日期：2001-02-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...

journal_title：Proteomics

authors： Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

更新日期：2006-12-01 00:00:00

abstract：:Spatial organisation of proteins according to their function plays an important role in the specificity of their molecular interactions. Emerging proteomics methods seek to assign proteins to sub-cellular locations by partial separation of organelles and computational analysis of protein abundance distributions among ...

journal_title：Proteomics

authors： Trotter MW,Sadowski PG,Dunkley TP,Groen AJ,Lilley KS

更新日期：2010-12-01 00:00:00

abstract：:Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...

journal_title：Proteomics

authors： Molle V,Leiba J,Zanella-Cléon I,Becchi M,Kremer L

更新日期：2010-11-01 00:00:00

abstract：:A proteome wide analysis was performed in Escherichia coli to identify the impact on protein N-termini of actinonin, an antibiotic specifically inhibiting peptide deformylase (PDF). A strategy and tool suite (SILProNaQ) was employed to provide large-scale quantitation of N-terminal modifications. In control conditions...

journal_title：Proteomics

authors： Bienvenut WV,Giglione C,Meinnel T

更新日期：2015-07-01 00:00:00

abstract：:Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...

journal_title：Proteomics

authors： Yu H,Hackenbroch L,Meyer FRL,Reiser J,Razzazi-Fazeli E,Nöbauer K,Besenfelder U,Vogl C,Brem G,Mayrhofer C

更新日期：2019-03-01 00:00:00

abstract：:Absolute protein concentration determination is becoming increasingly important in a number of fields including diagnostics, biomarker discovery and systems biology modeling. The recently introduced quantification concatamer methodology provides a novel approach to performing such determinations, and it has been appli...

journal_title：Proteomics

authors： Swainston N,Jameson D,Carroll K

更新日期：2011-01-01 00:00:00

abstract：:Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...

journal_title：Proteomics

authors： Ahmed N,Barker G,Oliva K,Garfin D,Talmadge K,Georgiou H,Quinn M,Rice G

更新日期：2003-10-01 00:00:00

abstract：:Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...

journal_title：Proteomics

authors： Uchiyama N,Kuno A,Tateno H,Kubo Y,Mizuno M,Noguchi M,Hirabayashi J

更新日期：2008-08-01 00:00:00

abstract：:Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...

journal_title：Proteomics

authors： Davoodi J,Kelly J,Gendron NH,MacKenzie AE

更新日期：2007-06-01 00:00:00

abstract：:In theory, proteases with broad cleavage specificity could be applied to digest protein samples to improve the phosphoproteomic analysis coverage. However, in practice this approach is seldom employed. This is because the identification of phosphopeptides without enzyme specificity by conventional database search stra...

journal_title：Proteomics

authors： Dong M,Ye M,Cheng K,Dong J,Zhu J,Qin H,Bian Y,Zou H

更新日期：2014-11-01 00:00:00

abstract：:Antibodies that recognize PTMs of histones play a central role in epigenetic proteomic research. Modification-specific antibodies are employed in chromatin immunoprecipitation, for Western blotting and during the immunoprecipitation steps for MS-based global proteomic analyses. Knowledge about the antibodies' off-targ...

journal_title：Proteomics

authors： Heubach Y,Planatscher H,Sommersdorf C,Maisch D,Maier J,Joos TO,Templin MF,Poetz O

更新日期：2013-03-01 00:00:00