Abstract:
:A proteomic analysis of a wild-type and of a phoB mutant showed that Vibrio cholerae expresses genes of two major regulons in response to phosphate starvation. The Pho regulon, expressed by the wild-type, allowed the cells to adapt to the new environment. Induction of the general stress regulon was mainly observed in the phoB mutant as a strategy to resist stress and survive. Some functions of the adaptative and survival responses play roles in the pathogenicity of the bacteria. Among the members of the Pho regulon, we found a porin described as an important factor for the intestinal colonisation. Other functions not obviously related to phosphate metabolism, expressed preferentially by the wild-type cells, have also been implicated in virulence. These findings might explain the lack of virulence of the phoB mutant. The Pho regulon picture of V. cholerae, however, will not be complete until minor members and membrane proteins are identified. Among the phosphate-starvation induced genes we have found 13 hypothetical ones and for some of them functions have been assigned. The majority of the genes identified here have not been described before, thus they could be used to expand the proteomic reference map of V. cholerae El Tor.
journal_name
Proteomicsjournal_title
Proteomicsauthors
von Krüger WM,Lery LM,Soares MR,de Neves-Manta FS,Batista e Silva CM,Neves-Ferreira AG,Perales J,Bisch PMdoi
10.1002/pmic.200500238subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1495-511issue
5eissn
1615-9853issn
1615-9861journal_volume
6pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500030
更新日期:2015-10-01 00:00:00
abstract::The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300645
更新日期:2004-02-01 00:00:00
abstract::The spring workshop of the HUPO-PSI convened in Siena to further progress the data standards which are already making an impact on data exchange and deposition in the field of proteomics. Separate work groups pushed forward existing XML standards for the exchange of Molecular Interaction data (PSI-MI, MIF) and Mass Sp...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200500626
更新日期:2005-09-01 00:00:00
abstract::We bring a "spectrum" of classical data mining and statistical analysis methods to bear on discrimination of two groups of spectra from 24 diseased and 17 normal patients. Our primary goal is to accurately estimate the generalizability of this small dataset. After an aggressive preprocessing step that reduces consider...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300516
更新日期:2003-09-01 00:00:00
abstract::Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201500290
更新日期:2016-01-01 00:00:00
abstract::Due to the limited applicability of conventional protein identification methods to the proteomes of organisms with unsequenced genomes, researchers have developed approaches to identify proteins using mass spectrometry and sequence similarity database searches. Both the integration of mass spectrometry with bioinforma...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200390004
更新日期:2003-01-01 00:00:00
abstract::Protein interactions have been at the focus of computational biology in recent years. In particular, interest has come from two different communities--structural and systems biology. Here, we will discuss key systems and structural biology methods that have been used for analysis and prediction of protein-protein inte...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100597
更新日期:2012-05-01 00:00:00
abstract::Plants exhibit resistance against incompatible pathogens, via localized and systemic responses as part of an integrated defense mechanism. To study the compatible and incompatible interactions between rice and bacteria, a proteomic approach was applied. Rice cv. Java 14 seedlings were inoculated with compatible (Xo743...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600470
更新日期:2006-11-01 00:00:00
abstract::Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated software, named IQuant, which integrates a postprocessing tool of protein identification and advanced statistical algorithms to process the MS/MS signals generated f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300361
更新日期:2014-10-01 00:00:00
abstract::During clinical mastitis in dairy cows, the quantity of milk produced decreases and the composition of the milk is altered. As the severity of inflammation associated with the disease increases, the chemical composition of milk approaches that of blood as a consequence of increased permeability of the blood mammary ba...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300723
更新日期:2004-07-01 00:00:00
abstract::Warm ischemia reperfusion (IR) injury of the liver is associated with changes in the expression and/or post-translational modification of numerous proteins. Only a few of these have been identified. We used 2-D DIGE to identify cytosolic proteins altered in the early stage of IR in an established rat model of segmenta...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100053
更新日期:2011-11-01 00:00:00
abstract::The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400072
更新日期:2014-10-01 00:00:00
abstract::The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertili...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800588
更新日期:2009-02-01 00:00:00
abstract::The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700508
更新日期:2008-05-01 00:00:00
abstract::In 2001, the German Federal Ministry of Education and Research (BMBF) initiated the National Genome Research Network (NGFN; www.ngfn.de) as a nation-wide multidisciplinary networking platform aiming at the analysis of common human diseases and aging. Within the NGFN the Human Brain Proteome Project (HBPP; www.smp-prot...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700980
更新日期:2008-03-01 00:00:00
abstract::In this report we introduce a new concept "proteomic trajectory mapping" for the investigation of a complex phenomenon underlying biological transformation and transition. We define proteomic trajectory to be the kinetic trace of protein expression and present a successful proteomic trajectory mapping of complex molec...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500813
更新日期:2006-06-01 00:00:00
abstract::Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400610
更新日期:2015-09-01 00:00:00
abstract::Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500129
更新日期:2006-03-01 00:00:00
abstract::Toxic oil syndrome (TOS) is a disease that appeared in Spain in 1981. It affected more than 20 000 people and produced over 300 deaths in the first 2 years. In this paper, a prospective study on the differences in gene expression in sera between a control versus a TOS-affected population, both originally exposed to th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300630
更新日期:2004-02-01 00:00:00
abstract::We compared peptide identification by database (DB) search methods with de novo sequencing results for proteomics study in an organism without genome sequence information. When the former was done by searching the Expressed Sequence Tag (EST) DB of the sample organism or the NCBI nonredundant (nr) protein DB of green ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300620
更新日期:2003-12-01 00:00:00
abstract::Determination of protein-protein interactions is an important component in assigning function and discerning the biological relevance of proteins within a broader cellular context. In vitro protein-protein interaction methodologies, including affinity chromatography, coimmunoprecipitation, and newer approaches such as...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500517
更新日期:2006-04-01 00:00:00
abstract::The flavonoid flavone contained in a variety of fruits and vegetables was identified as a very potent apoptosis inducer in human colonic cancer cells. In search of the molecular targets of flavone action in HT-29 cells we analyzed changes in mRNA and protein expression levels by proteomics and oligonucleotide array te...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300754
更新日期:2004-08-01 00:00:00
abstract::Mass spectrometry-based proteomics is used to gain insight into the abundance and subcellular localization of cellular signaling components, the composition of molecular complexes and the regulation of signaling pathways. Multicellular organisms have evolved signaling networks and fast responses to stimuli that can be...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000418
更新日期:2011-02-01 00:00:00
abstract::We report on a novel strategy for identification of specific sulfation motifs in chondroitin/dermatan sulfate (CS/DS) chain derived from decorin (Dcn), based on enzyme cleavage and multistage MS (MS(n)). Released CS/DS chains were digested with chondroitin B and in parallel with AC I lyases to obtain oligosaccharides ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800440
更新日期:2009-07-01 00:00:00
abstract::Calcineurin is a heterodimeric serine/threonine protein phosphatase, important for many cellular processes such as T-cell regulation, cardiac hypertrophy and kidney development. We previously reported the characterization of Caenorhabditis elegans calcineurin mutants as providing a simple but excellent genetic model s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500315
更新日期:2006-02-01 00:00:00
abstract::The field of biochemistry is currently faced with the enormous challenge of assigning functional significance to more than thirty thousand predicted protein products encoded by the human genome. In order to accomplish this daunting task, methods will be required that facilitate the global analysis of proteins in compl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200109)1:9<1067::AID-PROT1067>3.
更新日期:2001-09-01 00:00:00
abstract::Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either (13) C-naphthalene or (13) C-fluorene ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200569
更新日期:2013-10-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs. A large-scale outbreak of PED occurred in China in 2010, with PEDV emerging in the United States in 2013 and spreading rapidly, posing significant economic and p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400458
更新日期:2015-06-01 00:00:00
abstract::We performed phosphoproteome analysis of proteins from the extremely thermophilic Gram-negative eubacterium Thermus thermophilus HB8 using gel-free mass spectrometric method. We identified 52 phosphopeptides from 48 proteins and determined 46 phosphorylation sites: 30 on serine, 12 on threonine, and 4 on tyrosine. The...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100573
更新日期:2012-05-01 00:00:00
abstract::Targeted quantitative proteomic analyses aim at systematically measuring the abundance of proteins in large sets of samples, without biases or missing values. One typical implementation is the verification of biomarker candidates in bodily fluids, which measures extended lists of validated transitions using triple qua...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201400450
更新日期:2015-03-01 00:00:00