Abstract:
:We compared peptide identification by database (DB) search methods with de novo sequencing results for proteomics study in an organism without genome sequence information. When the former was done by searching the Expressed Sequence Tag (EST) DB of the sample organism or the NCBI nonredundant (nr) protein DB of green plants using either the MASCOT or SEQUEST software program, it was confirmed that the former is as accurate as the latter. Peptides identified from EST DB were twice as many as those from the nr protein DB, in spite of the fact that the EST DB has less data (26 222 EST) than the NCBI nr protein DB (224 238). This study demonstrates that EST DB with tandem mass spectra can be used reliably for high-throughput proteomics studies in an organism without genome information.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Kwon KH,Kim M,Kim JY,Kim KW,Kim SI,Park YM,Yoo JSdoi
10.1002/pmic.200300620subject
Has Abstractpub_date
2003-12-01 00:00:00pages
2305-9issue
12eissn
1615-9853issn
1615-9861journal_volume
3pub_type
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