Abstract:
:Cells are a fundamental unit of life, and the ability to study the phenotypes and behaviors of individual cells is crucial to understanding the workings of complex biological systems. Cell phenotypes (epigenomic, transcriptomic, proteomic, and metabolomic) exhibit dramatic heterogeneity between and within the different cell types and states underlying cellular functional diversity. Cell genotypes can also display heterogeneity throughout an organism, in the form of somatic genetic variation-most notably in the emergence and evolution of tumors. Recent technical advances in single-cell isolation and the development of omics approaches sensitive enough to reveal these aspects of cell identity have enabled a revolution in the study of multicellular systems. In this review, we discuss the technologies available to resolve the genomes, epigenomes, transcriptomes, proteomes, and metabolomes of single cells from a wide variety of living systems.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Mincarelli L,Lister A,Lipscombe J,Macaulay ICdoi
10.1002/pmic.201700312subject
Has Abstractpub_date
2018-09-01 00:00:00pages
e1700312issue
18eissn
1615-9853issn
1615-9861journal_volume
18pub_type
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