Abstract:
:Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate in the surrogate E. coli, followed by MS analysis in a single-step procedure. The performance of this strategy is illustrated using two distinct proteins from Mycobacterium tuberculosis as model substrates, the GroEL2 and HspX chaperones. A comparative analysis with a standard method that includes mass spectrometry analysis of in vitro phosphorylated substrates is also addressed.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Molle V,Leiba J,Zanella-Cléon I,Becchi M,Kremer Ldoi
10.1002/pmic.201000316subject
Has Abstractpub_date
2010-11-01 00:00:00pages
3910-5issue
21eissn
1615-9853issn
1615-9861journal_volume
10pub_type
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