Novel eye-specific calmodulin methylation characterized by protein mapping in Drosophila melanogaster.

abstract：:A method for quantitative proteomic analysis based on the selective isolation of multiply charged peptides (RH peptides) containing arginine and histidine residues is described. Two pools of proteins are digested in tandem with lysyl-endopeptidase and trypsin and the primary amino groups of proteolytic peptides are se...

journal_title：Proteomics

authors： Sánchez A,González LJ,Betancourt L,Gil J,Besada V,Fernández-de-Cossío J,Rodríguez-Ulloa A,Marrero K,Alvarez F,Fando R,Padrón G

更新日期：2006-08-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry (MS/MS) were used to determine the multiple acetylation sites in the histone acetyltransferase (HAT): p300-HAT. Partial cleavage of the peptides containing acetylated lysine residues by trypsin provided a set of nested sequences...

journal_title：Proteomics

authors： Wang D,Thompson P,Cole PA,Cotter RJ

更新日期：2005-06-01 00:00:00

abstract：:Since detergent-resistant lipid rafts play important roles in the signal transduction for myogenesis, their comprehensive proteomic analysis could provide new insights to understand their function in myotubes. Here, the detergent-resistant lipid rafts were isolated from C2C12 myotubes and analyzed by capillary RPLC/MS...

journal_title：Proteomics

authors： Kim BW,Lee JW,Choo HJ,Lee CS,Jung SY,Yi JS,Ham YM,Lee JH,Hong J,Kang MJ,Chi SG,Hyung SW,Lee SW,Kim HM,Cho BR,Min DS,Yoon G,Ko YG

更新日期：2010-07-01 00:00:00

abstract：:This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. T...

journal_title：Proteomics

authors： Heo SH,Choi JH,Kim YM,Jung CW,Lee J,Jin HY,Kim GH,Lee BH,Shin CH,Yoo HW

更新日期：2013-04-01 00:00:00

abstract：:Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high-throughput ...

journal_title：Proteomics

authors： Dam S,Dyrlund TF,Ussatjuk A,Jochimsen B,Nielsen K,Goffard N,Ventosa M,Lorentzen A,Gupta V,Andersen SU,Enghild JJ,Ronson CW,Roepstorff P,Stougaard J

更新日期：2014-02-01 00:00:00

abstract：:A characteristic of two-dimensional proteomics gels is a general bimodal distribution of isoelectric (pI) values. Discussion of this feature has focussed on the balance of acidic and basic ionisable residues, and potential relationships between pI distributions and organism classification or protein subcellular locati...

journal_title：Proteomics

authors： Chan P,Lovrić J,Warwicker J

更新日期：2006-06-01 00:00:00

abstract：:The glycan structures of the major and more than ten minor populated isoforms of antithrombin (AT) were determined after separation of the isoforms by IEF using IPG strips. The bands excised from the gel were reduced, derivatized by iodoacetamide and submitted to tryptic digestion. The digest was analyzed by RP-HPLC-E...

journal_title：Proteomics

authors： Plematl A,Demelbauer UM,Josic D,Rizzi A

更新日期：2005-10-01 00:00:00

abstract：:Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...

journal_title：Proteomics

authors： Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

更新日期：2005-04-01 00:00:00

abstract：:The quantification of changes in protein abundance in complex biological specimens is essential for proteomic studies in basic and applied research. Here we report on the development and validation of the DeepQuanTR software for identification and quantification of differentially expressed proteins using LC-MALDI-MS. ...

journal_title：Proteomics

authors： Fugmann T,Neri D,Roesli C

更新日期：2010-07-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrP(TSE)) of the host-encoded cellular prion protein (PrP(C)). PrP(TSE) has a central role in the pathogenesis of the disease but other factors are likely involved ...

journal_title：Proteomics

authors： Giorgi A,Di Francesco L,Principe S,Mignogna G,Sennels L,Mancone C,Alonzi T,Sbriccoli M,De Pascalis A,Rappsilber J,Cardone F,Pocchiari M,Maras B,Schininà ME

更新日期：2009-08-01 00:00:00

abstract：:Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

journal_title：Proteomics

authors： Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

更新日期：2006-02-01 00:00:00

abstract：:Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...

journal_title：Proteomics

authors： Kim SY,Joglekar MV,Hardikar AA,Phan TH,Khanal D,Tharkar P,Limantoro C,Johnson J,Kalionis B,Chrzanowski W

更新日期：2019-09-01 00:00:00

abstract：:During clinical mastitis in dairy cows, the quantity of milk produced decreases and the composition of the milk is altered. As the severity of inflammation associated with the disease increases, the chemical composition of milk approaches that of blood as a consequence of increased permeability of the blood mammary ba...

journal_title：Proteomics

authors： Hogarth CJ,Fitzpatrick JL,Nolan AM,Young FJ,Pitt A,Eckersall PD

更新日期：2004-07-01 00:00:00

abstract：:The explosion of site- and context-specific in vivo phosphorylation events presents a potentially rich source of biological knowledge and calls for novel data analysis and modeling paradigms. Perhaps the most immediate challenge is delineating detected phosphorylation sites to their effector kinases. This is important...

journal_title：Proteomics

authors： Tan CS,Linding R

更新日期：2009-12-01 00:00:00

abstract：:Basal-like breast cancers are commonly negative for expression of estrogen and progesterone receptors and HER-2 (triple-negative breast cancer), which makes this subtype of breast cancers more aggressive and less responsive to standard treatment. We have applied a small-scale chemical proteomics method using bisindoly...

journal_title：Proteomics

authors： Dolai S,Xu Q,Liu F,Molloy MP

更新日期：2011-07-01 00:00:00

abstract：:The rice heterotrimeric G-protein complex, a guanine-nucleotide-dependent on-off switch, mediates vital cellular processes and responses to biotic and abiotic stress. Exchange of bound GDP (resting state) for GTP (active state) is spontaneous in plants including rice and thus there is no need for promoting guanine nuc...

journal_title：Proteomics

authors： Biswal AK,McConnell EW,Werth EG,Lo SF,Yu SM,Hicks LM,Jones AM

更新日期：2019-05-01 00:00:00

abstract：:Most proteins in all organisms undergo crucial N-terminal modifications involving N-terminal methionine excision, N-alpha-acetylation or N-myristoylation (N-Myr), or S-palmitoylation. We investigated the occurrence of these poorly annotated but essential modifications in proteomes, focusing on eukaryotes. Experimental...

journal_title：Proteomics

authors： Martinez A,Traverso JA,Valot B,Ferro M,Espagne C,Ephritikhine G,Zivy M,Giglione C,Meinnel T

更新日期：2008-07-01 00:00:00

abstract：:Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...

journal_title：Proteomics

authors： Josic D,Clifton JG

更新日期：2007-08-01 00:00:00

abstract：:Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylati...

journal_title：Proteomics

authors： Wang T,Guan W,Du Y,Xu Y,He Z,Zhang Y,Kang C,Wan X,Chi X,Sun K,Zhang X

更新日期：2021-01-24 00:00:00

abstract：:The transfection of glycoprotein glycosyltransferase genes into cells leads to modification of both the structure and function of the glycoproteins and as a result, changes in glycome patterns. N-glycan branching enzymes hold some promise as a model system for the identification of glycome patterns. Both N-acetylgluco...

journal_title：Proteomics

authors： Taniguchi N,Ekuni A,Ko JH,Miyoshi E,Ikeda Y,Ihara Y,Nishikawa A,Honke K,Takahashi M

更新日期：2001-02-01 00:00:00

abstract：:Blood plasma and serum are very useful samples for the detection, identification and quantitation of proteins associated with both health and disease. However, analysis of plasma and serum is a challenge because traces of interesting polypeptides and proteins can be dominated by the very high concentration of albumin ...

journal_title：Proteomics

authors： Baussant T,Bougueleret L,Johnson A,Rogers J,Menin L,Hall M,Aberg PM,Rose K

更新日期：2005-03-01 00:00:00

abstract：:Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...

journal_title：Proteomics

authors： Aziz MM,Maleki M,Rueda L,Raza M,Banerjee S

更新日期：2011-10-01 00:00:00

abstract：:As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...

journal_title：Proteomics

authors： Wang W,Guo T,Song T,Lee CS,Balgley BM

更新日期：2007-04-01 00:00:00

abstract：:A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differenti...

journal_title：Proteomics

authors： Winter D,Seidler J,Kugelstadt D,Derrer B,Kappes B,Lehmann WD

更新日期：2010-04-01 00:00:00

abstract：:Oligogalacturonides (OGs) are elicitors of plant defence responses released from the homogalacturonan of the plant cell wall during the attack by pathogenic micro-organisms. The signalling pathway mediated by OGs remains poorly understood, and no proteins involved in their signal perception and transduction have yet b...

journal_title：Proteomics

authors： Casasoli M,Spadoni S,Lilley KS,Cervone F,De Lorenzo G,Mattei B

更新日期：2008-03-01 00:00:00

abstract：:In this study we investigated the cellular response to the application of high hydrostatic pressure. High pressure is increasingly used for food preservation. With high resolution 2-D electrophoresis we compared the protein patterns of atmospherically grown Lactobacillus sanfranciscensis with those pressure treated up...

journal_title：Proteomics

authors： Drews O,Weiss W,Reil G,Parlar H,Wait R,Görg A

更新日期：2002-06-01 00:00:00

abstract：:The tuber of potato (Solanum tuberosum) is commonly used as a model for underground storage organs. In this study, changes in the proteome were followed from tuberization, through tuber development and storage into the sprouting phase. Data interrogation using principal component analysis was able to clearly discrimin...

journal_title：Proteomics

authors： Lehesranta SJ,Davies HV,Shepherd LV,Koistinen KM,Massat N,Nunan N,McNicol JW,Kärenlampi SO

更新日期：2006-11-01 00:00:00

abstract：:Normal Chinese Liver Proteome Expression Profile is one of the major parts of Human Liver Proteome Project. Before starting the studies, it is necessary to examine the interindividual variation of normal liver proteome and evaluate the minimal size of samples for proteomic analysis. In this study, normal liver samples...

journal_title：Proteomics

authors： Zhang X,Guo Y,Song Y,Sun W,Yu C,Zhao X,Wang H,Jiang H,Li Y,Qian X,Jiang Y,He F

更新日期：2006-10-01 00:00:00

abstract：:Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an ...

journal_title：Proteomics

authors： Schueder F,Unterauer EM,Ganji M,Jungmann R

更新日期：2020-10-08 00:00:00

abstract：:The flavonoid flavone contained in a variety of fruits and vegetables was identified as a very potent apoptosis inducer in human colonic cancer cells. In search of the molecular targets of flavone action in HT-29 cells we analyzed changes in mRNA and protein expression levels by proteomics and oligonucleotide array te...

journal_title：Proteomics

authors： Herzog A,Kindermann B,Döring F,Daniel H,Wenzel U

更新日期：2004-08-01 00:00:00