Identification by 2-D DIGE of apoplastic proteins regulated by oligogalacturonides in Arabidopsis thaliana.

abstract：:Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...

journal_title：Proteomics

authors： Erce MA,Pang CN,Hart-Smith G,Wilkins MR

更新日期：2012-02-01 00:00:00

abstract：:The glycan structures of the major and more than ten minor populated isoforms of antithrombin (AT) were determined after separation of the isoforms by IEF using IPG strips. The bands excised from the gel were reduced, derivatized by iodoacetamide and submitted to tryptic digestion. The digest was analyzed by RP-HPLC-E...

journal_title：Proteomics

authors： Plematl A,Demelbauer UM,Josic D,Rizzi A

更新日期：2005-10-01 00:00:00

abstract：:Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (...

journal_title：Proteomics

authors： Huang QY,Huang HQ

更新日期：2011-09-01 00:00:00

abstract：:An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to F...

journal_title：Proteomics

authors： Noga MJ,Lewandowski JJ,Suder P,Silberring J

更新日期：2005-11-01 00:00:00

abstract：:To exploit advances in proteomics for drug discovery, high-throughout methods for target validation that directly address the cellular roles of proteins are required. To do this, we have characterized fluorophore-assisted light inactivation (FALI) which uses coherent or diffuse light targeted by fluorescein-labeled pr...

journal_title：Proteomics

authors： Beck S,Sakurai T,Eustace BK,Beste G,Schier R,Rudert F,Jay DG

更新日期：2002-03-01 00:00:00

abstract：:We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...

journal_title：Proteomics

authors： Goodwin RJ,Dungworth JC,Cobb SR,Pitt AR

更新日期：2008-09-01 00:00:00

abstract：:LTQ Orbitrap data analyzed with ProteinPilot can be further improved by MaxQuant raw data processing, which utilizes precursor-level high mass accuracy data for peak processing and MGF creation. In particular, ProteinPilot results from MaxQuant-processed peaklists for Orbitrap data sets resulted in improved spectral u...

journal_title：Proteomics

authors： Jagtap P,Bandhakavi S,Higgins L,McGowan T,Sa R,Stone MD,Chilton J,Arriaga EA,Seymour SL,Griffin TJ

更新日期：2012-06-01 00:00:00

abstract：:Protein quantification at proteome-wide scale is an important aim, enabling insights into fundamental cellular biology and serving to constrain experiments and theoretical models. While proteome-wide quantification is not yet fully routine, many datasets approaching proteome-wide coverage are becoming available throug...

journal_title：Proteomics

authors： Wang M,Herrmann CJ,Simonovic M,Szklarczyk D,von Mering C

更新日期：2015-09-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a major complication of chronic viral hepatitis C. Therapy for HCC is still disappointing. It is thus of great importance to identify novel HCC markers for early detection of the disease, and tumor-specific proteins as potential therapeutic targets. We have used a proteomic approach t...

journal_title：Proteomics

authors： Blanc JF,Lalanne C,Plomion C,Schmitter JM,Bathany K,Gion JM,Bioulac-Sage P,Balabaud C,Bonneu M,Rosenbaum J

更新日期：2005-09-01 00:00:00

abstract：:MDM2 is a multidomain protein that functions as an E3 ubiquitin ligase, transcription repressor, mRNA-binding protein, translation factor, and molecular chaperone. The small molecule Nutlin-3 has been engineered to bind to the N-terminal hydrophobic pocket domain of MDM2. This binding of Nutlin-3 has two consequences:...

journal_title：Proteomics

authors： Hernychova L,Man P,Verma C,Nicholson J,Sharma CA,Ruckova E,Teo JY,Ball K,Vojtesek B,Hupp TR

更新日期：2013-08-01 00:00:00

abstract：:The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...

journal_title：Proteomics

authors： Kasyapa C,Gu TL,Nagarajan L,Polakiewicz R,Cowell JK

更新日期：2009-08-01 00:00:00

abstract：:Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...

journal_title：Proteomics

authors： Grozav AG,Willard BB,Kozuki T,Chikamori K,Micluta MA,Petrescu AJ,Kinter M,Ganapathi R,Ganapathi MK

更新日期：2011-03-01 00:00:00

abstract：:Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of these channels is tightly controlled by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signaling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP2 ), Ca2+ /calmodulin, and pho...

journal_title：Proteomics

authors： Erdem FA,Salzer I,Heo S,Chen WQ,Jung G,Lubec G,Boehm S,Yang JW

更新日期：2017-10-01 00:00:00

abstract：:Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. I...

journal_title：Proteomics

authors： Gilbert I,Schiffmann S,Rubenwolf S,Jensen K,Mai T,Albrecht C,Lankenau A,Beste G,Blank K,Gaub HE,Clausen-Schaumann H

更新日期：2004-05-01 00:00:00

abstract：:We here present a user-friendly and extremely lightweight tool that can serve as a stand-alone front-end for the Open MS Search Algorithm (OMSSA) search engine, or that can directly be used as part of an informatics processing pipeline for MS driven proteomics. The OMSSA graphical user interface (OMSSAGUI) tool is wri...

journal_title：Proteomics

authors： Tharakan R,Martens L,Van Eyk JE,Graham DR

更新日期：2008-06-01 00:00:00

abstract：:Advanced glycation endproduct (AGE) formation is an important mechanism for protein deterioration during diabetic complications and ageing. The effects on AGE formation following dihydroxyacetone (DHA) stress were studied in two model organisms, the yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegan...

journal_title：Proteomics

authors： Molin M,Pilon M,Blomberg A

更新日期：2007-10-01 00:00:00

abstract：:The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...

journal_title：Proteomics

authors： Jorba N,Juarez S,Torreira E,Gastaminza P,Zamarreño N,Albar JP,Ortín J

更新日期：2008-05-01 00:00:00

abstract：:Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such a...

journal_title：Proteomics

authors： Banfi C,Brioschi M,Wait R,Begum S,Gianazza E,Fratto P,Polvani G,Vitali E,Parolari A,Mussoni L,Tremoli E

更新日期：2006-03-01 00:00:00

abstract：:Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylati...

journal_title：Proteomics

authors： Wang T,Guan W,Du Y,Xu Y,He Z,Zhang Y,Kang C,Wan X,Chi X,Sun K,Zhang X

更新日期：2021-01-24 00:00:00

abstract：:Urinary microvesicles constitute a rich source of membrane-bound and intracellular proteins that may provide important clues of pathophysiological mechanisms in renal disease. In the current study, we analyzed and compared the proteome of urinary microvesicles from patients with idiopathic membranous nephropathy (iMN)...

journal_title：Proteomics

authors： Rood IM,Merchant ML,Wilkey DW,Zhang T,Zabrouskov V,van der Vlag J,Dijkman HB,Willemsen BK,Wetzels JF,Klein JB,Deegens JK

更新日期：2015-11-01 00:00:00

abstract：:When the p-value is set at <0.05 in statistical group comparisons, a 5% rate of "false significant" results is expected. In order to test the reliability of our 2-DE method, we loaded each of 24 gels with equal-sized samples (200 mug protein from pooled rat brain, pH 4-7, stained with ruthenium fluorescent stain for v...

journal_title：Proteomics

authors： Fuxius S,Eravci M,Broedel O,Weist S,Mansmann U,Eravci S,Baumgartner A

更新日期：2008-05-01 00:00:00

abstract：:The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen prepa...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Nebendahl M,Janssen O,Petersen A

更新日期：2010-12-01 00:00:00

abstract：:Plant secretomics is a newly emerging area of the plant proteomics field. It basically describes the global study of secreted proteins into the extracellular space of plant cell or tissue at any given time and under certain conditions through various secretory mechanisms. A combination of biochemical, proteomics and b...

journal_title：Proteomics

authors： Agrawal GK,Jwa NS,Lebrun MH,Job D,Rakwal R

更新日期：2010-02-01 00:00:00

abstract：:Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of...

journal_title：Proteomics

authors： Wowk PF,Zardo ML,Miot HT,Goldenberg S,Carvalho PC,Mörking PA

更新日期：2017-08-01 00:00:00

abstract：:Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. Ho...

journal_title：Proteomics

authors： Zavan L,Bitto NJ,Johnston EL,Greening DW,Kaparakis-Liaskos M

更新日期：2019-01-01 00:00:00

abstract：:Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...

journal_title：Proteomics

authors： Schuemie M,Chichester C,Lisacek F,Coute Y,Roes PJ,Sanchez JC,Kors J,Mons B

更新日期：2007-03-01 00:00:00

abstract：:Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...

journal_title：Proteomics

authors： Gao Y,Lee H,Kwon OK,Cheng Z,Tan M,Kim KT,Lee S

更新日期：2019-05-01 00:00:00

abstract：:Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells...

journal_title：Proteomics

authors： Khare T,Esteve-Núñez A,Nevin KP,Zhu W,Yates JR 3rd,Lovley D,Giometti CS

更新日期：2006-01-01 00:00:00

abstract：:Normal sera contain a large number of naturally occurring autoantibodies which can mask important disease-associated ones. Western blotting has evolved as the most important tool to demonstrate autoantibodies in autoimmune diseases, because of its ability to simultaneous screening for a wide spectrum of different anti...

journal_title：Proteomics

authors： Grus FH,Joachim SC,Pfeiffer N

更新日期：2003-06-01 00:00:00

abstract：:With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...

journal_title：Proteomics

authors： Bereman MS

更新日期：2015-03-01 00:00:00