Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment.

abstract：:Most proteins in all organisms undergo crucial N-terminal modifications involving N-terminal methionine excision, N-alpha-acetylation or N-myristoylation (N-Myr), or S-palmitoylation. We investigated the occurrence of these poorly annotated but essential modifications in proteomes, focusing on eukaryotes. Experimental...

journal_title：Proteomics

authors： Martinez A,Traverso JA,Valot B,Ferro M,Espagne C,Ephritikhine G,Zivy M,Giglione C,Meinnel T

更新日期：2008-07-01 00:00:00

abstract：:Over the past decade, considerable progress has been made with respect to the analytical methods for analysis of glycans from biological sources. Regardless of the specific methods that are used, glycan analysis includes isolation, identification, and quantitation. Derivatization is indispensable to increase their ide...

journal_title：Proteomics

authors： Yang S,Rubin A,Eshghi ST,Zhang H

更新日期：2016-01-01 00:00:00

abstract：:A proteomics approach has been used to study changes in protein abundance during leaf senescence in white clover. Changes in cell ultrastructure were also examined using transmission electron microscopy. The most obvious ultrastructural changes during senescence occurred in chloroplasts, with progressive loss of thyla...

journal_title：Proteomics

authors： Wilson KA,McManus MT,Gordon ME,Jordan TW

更新日期：2002-09-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:The International Plant Proteomics Organization (INPPO) is a global platform of the plant proteomics community or, more generally, the scientific community that uses proteomics to address plant biology. Organizing an international conference is one of its initiatives to promote plant proteomics by involving and gather...

journal_title：Proteomics

authors： Lüthje S,Renaut J,Job D,Hajduch M,Carpentier S,Sarkar A,Agrawal R,Dunn MJ,Rakwal R,Agrawal GK

更新日期：2015-05-01 00:00:00

abstract：:Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...

journal_title：Proteomics

authors： Kamita M,Mori T,Sakai Y,Ito S,Gomi M,Miyamoto Y,Harada A,Niida S,Yamada T,Watanabe K,Ono M

更新日期：2015-05-01 00:00:00

abstract：:Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...

journal_title：Proteomics

authors： Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RD

更新日期：2012-04-01 00:00:00

abstract：:Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...

journal_title：Proteomics

authors： Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

更新日期：2012-06-01 00:00:00

abstract：:Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...

journal_title：Proteomics

authors： Grozav AG,Willard BB,Kozuki T,Chikamori K,Micluta MA,Petrescu AJ,Kinter M,Ganapathi R,Ganapathi MK

更新日期：2011-03-01 00:00:00

abstract：:Oligogalacturonides (OGs) are elicitors of plant defence responses released from the homogalacturonan of the plant cell wall during the attack by pathogenic micro-organisms. The signalling pathway mediated by OGs remains poorly understood, and no proteins involved in their signal perception and transduction have yet b...

journal_title：Proteomics

authors： Casasoli M,Spadoni S,Lilley KS,Cervone F,De Lorenzo G,Mattei B

更新日期：2008-03-01 00:00:00

abstract：:FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...

journal_title：Proteomics

authors： Gattiker A,Bienvenut WV,Bairoch A,Gasteiger E

更新日期：2002-10-01 00:00:00

abstract：:The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...

journal_title：Proteomics

authors： Bigeard J,Rayapuram N,Bonhomme L,Hirt H,Pflieger D

更新日期：2014-10-01 00:00:00

abstract：:ProDaC (Proteomics Data Collection), a "Coordination Action" within the 6(th) EU framework programme, was created to support the collection, distribution and public availability of data from proteomics experiments. Within the consortium standards are created and maintained enabling an extensive data collection within ...

journal_title：Proteomics

authors： Eisenacher M,Kohl M,Martens L,Barsnes H,Hardt T,Levander F,Häkkinen J,Apweiler R,Meyer HE,Stephan C

更新日期：2009-01-01 00:00:00

abstract：:Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs)...

journal_title：Proteomics

authors： Evans J,Rai A,Nguyen HPT,Poh QH,Elglass K,Simpson RJ,Salamonsen LA,Greening DW

更新日期：2019-12-01 00:00:00

abstract：:There is ample evidence for the involvement of protein phosphorylation on serine/threonine/tyrosine in bacterial signaling and regulation, but very few exact phosphorylation sites have been experimentally determined. Recently, gel-free high accuracy MS studies reported over 150 phosphorylation sites in two bacterial m...

journal_title：Proteomics

authors： Miller ML,Soufi B,Jers C,Blom N,Macek B,Mijakovic I

更新日期：2009-01-01 00:00:00

abstract：:FBXO25 is one of the 68 human F-box proteins that serve as specificity factors for a family of ubiquitin ligases composed of s-phase-kinase associated protein 1, really interesting new gene-box 1, Cullin 1, and F-box protein (SCF1) that are involved in targeting proteins for destruction across the ubiquitin proteasome...

journal_title：Proteomics

authors： Teixeira FR,Yokoo S,Gartner CA,Manfiolli AO,Baqui MM,Assmann EM,Maragno AL,Yu H,de Lanerolle P,Kobarg J,Gygi SP,Gomes MD

更新日期：2010-08-01 00:00:00

abstract：:Calcineurin is a heterodimeric serine/threonine protein phosphatase, important for many cellular processes such as T-cell regulation, cardiac hypertrophy and kidney development. We previously reported the characterization of Caenorhabditis elegans calcineurin mutants as providing a simple but excellent genetic model s...

journal_title：Proteomics

authors： Ahn DH,Singaravelu G,Lee S,Ahnn J,Shim YH

更新日期：2006-02-01 00:00:00

abstract：:Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...

journal_title：Proteomics

authors： Tsai JJ,Kuo HC,Lee KF,Tsai TH

更新日期：2015-11-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. Miniaturization of IMAC column is essential for the analysis of a small amount of sample. Nanoscale IMAC column was prepared by chemical modification of...

journal_title：Proteomics

authors： Feng S,Pan C,Jiang X,Xu S,Zhou H,Ye M,Zou H

更新日期：2007-02-01 00:00:00

abstract：:Off-line coupling of capillary IEF (CIEF) with matrix-assisted laser desorption/ionization mass spectrometry was utilized for the analysis of human blood serum. Serum proteins were initially separated by CIEF, and fractions of the isoelectric separation were eluted sequentially to a MALDI-TOF MS sample target. During ...

journal_title：Proteomics

authors： Crowley TA,Hayes MA

更新日期：2005-09-01 00:00:00

abstract：:Pathogenic mycobacteria persist and replicate within phagosomes of host phagocytes by inhibiting phagosome maturation at an early endosome stage. The molecular basis for this behavior is not understood. To identify proteins of Mycobacterium tuberculosis unique to the intraphagosomal phase, mycobacteria were purified f...

journal_title：Proteomics

authors： Mattow J,Siejak F,Hagens K,Becher D,Albrecht D,Krah A,Schmidt F,Jungblut PR,Kaufmann SH,Schaible UE

更新日期：2006-04-01 00:00:00

abstract：:Determination of protein-protein interactions is an important component in assigning function and discerning the biological relevance of proteins within a broader cellular context. In vitro protein-protein interaction methodologies, including affinity chromatography, coimmunoprecipitation, and newer approaches such as...

journal_title：Proteomics

authors： Howell JM,Winstone TL,Coorssen JR,Turner RJ

更新日期：2006-04-01 00:00:00

abstract：:Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, we provide a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson-Golabi-Behmel Syndrome (SGBS) cells. The SGBS cells are a...

journal_title：Proteomics

authors： Kalkhof S,Krieg L,Büttner P,Wabitsch M,Küntzel C,Friebe D,Landgraf K,Hanschkow M,Schubert K,Kiess W,Krohn K,Blüher M,von Bergen M,Körner A

更新日期：2020-05-08 00:00:00

abstract：:The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...

journal_title：Proteomics

authors： Griese M,Noss J,von Bredow C

更新日期：2002-06-01 00:00:00

abstract：:Recombinant human growth hormone (rhGH) is used for the treatment of several disorders. Structural integrity of rhGH is of critical importance for its clinical use and modifications thereof may act as markers in situations such as rhGH doping, as illegal rhGH-abuse in sports is of increasing interest. In the current s...

journal_title：Proteomics

authors： Hepner F,Csaszar E,Roitinger E,Pollak A,Lubec G

更新日期：2006-02-01 00:00:00

abstract：:Data-independent acquisition (DIA) offers several advantages over data-dependent acquisition (DDA) schemes for characterizing complex protein digests analyzed by LC-MS/MS. In contrast to the sequential detection, selection, and analysis of individual ions during DDA, DIA systematically parallelizes the fragmentation o...

journal_title：Proteomics

authors： Bilbao A,Varesio E,Luban J,Strambio-De-Castillia C,Hopfgartner G,Müller M,Lisacek F

更新日期：2015-03-01 00:00:00

abstract：:Despite affecting up to 70% of HIV-positive patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on...

journal_title：Proteomics

authors： Ganief T,Gqamana P,Garnett S,Hoare J,Stein DJ,Joska J,Soares N,Blackburn JM

更新日期：2017-03-01 00:00:00

abstract：:The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic dig...

journal_title：Proteomics

authors： Gundry RL,Tchernyshyov I,Sheng S,Tarasova Y,Raginski K,Boheler KR,Van Eyk JE

更新日期：2010-07-01 00:00:00

abstract：:Despite increasing evidence of behavioural manipulation of their vectors by pathogens, the underlying mechanisms causing infected vectors to act in ways that benefit pathogen transmission remain enigmatic in most cases. Here, 2-D DIGE coupled with MS were employed to analyse and compare the head proteome of mosquitoes...

journal_title：Proteomics

authors： Lefevre T,Thomas F,Schwartz A,Levashina E,Blandin S,Brizard JP,Le Bourligu L,Demettre E,Renaud F,Biron DG

更新日期：2007-06-01 00:00:00

abstract：:O-GlcNAcylation is a dynamic PTM of nuclear and cytoplasmic proteins, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase, which catalyze the addition and removal of O-GlcNAc, respectively. This modification is associated with glucose metabolism, which plays important roles in many diseases including cancer. Altho...

journal_title：Proteomics

authors： Champattanachai V,Netsirisawan P,Chaiyawat P,Phueaouan T,Charoenwattanasatien R,Chokchaichamnankit D,Punyarit P,Srisomsap C,Svasti J

更新日期：2013-07-01 00:00:00