Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.

abstract：:High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...

journal_title：Proteomics

authors： Majoul T,Bancel E,Triboï E,Ben Hamida J,Branlard G

更新日期：2003-02-01 00:00:00

abstract：:The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...

journal_title：Proteomics

authors： Kasyapa C,Gu TL,Nagarajan L,Polakiewicz R,Cowell JK

更新日期：2009-08-01 00:00:00

abstract：:As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...

journal_title：Proteomics

authors： Wang W,Guo T,Song T,Lee CS,Balgley BM

更新日期：2007-04-01 00:00:00

abstract：:Current treatments for advanced prostate cancer focus on inhibition of the androgen receptor (AR) by androgen deprivation therapy (ADT). However, complex interactions mediated by tumor suppressors, oncogenes, aberrations of AR expression, or de novo androgen production have been shown to induce the adaptive response o...

journal_title：Proteomics

authors： Soekmadji C,Rockstroh A,Ramm GA,Nelson CC,Russell PJ

更新日期：2017-12-01 00:00:00

abstract：:To better understand the mechanism underlying hepatocellular carcinoma (HCC) metastasis and to search for potential markers for HCC prognosis, differential proteome analysis on two HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional (2-D) gel electrophor...

journal_title：Proteomics

authors： Ding SJ,Li Y,Shao XX,Zhou H,Zeng R,Tang ZY,Xia QC

更新日期：2004-04-01 00:00:00

abstract：:Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells...

journal_title：Proteomics

authors： Khare T,Esteve-Núñez A,Nevin KP,Zhu W,Yates JR 3rd,Lovley D,Giometti CS

更新日期：2006-01-01 00:00:00

abstract：:Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...

journal_title：Proteomics

authors： Josic D,Clifton JG

更新日期：2007-08-01 00:00:00

abstract：:Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...

journal_title：Proteomics

authors： Yu H,Hackenbroch L,Meyer FRL,Reiser J,Razzazi-Fazeli E,Nöbauer K,Besenfelder U,Vogl C,Brem G,Mayrhofer C

更新日期：2019-03-01 00:00:00

abstract：:Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...

journal_title：Proteomics

authors： Ahrné E,Müller M,Lisacek F

更新日期：2010-02-01 00:00:00

abstract：:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...

journal_title：Proteomics

authors： Corrigan L,Jefferies C,Clive Lee T,Daly J

更新日期：2011-08-01 00:00:00

abstract：:To develop a standard method for separating highly basic proteins in mammalian cells, we established a 2-D LC separation system coupled with chromatofocusing/nonporous RP column chromatography (CF/NPRPC) in a ProteomeLab PF2D system. After standardizing conditions for 2-D LC, a 2-D liquid protein map of uninfected mac...

journal_title：Proteomics

authors： Shin YK,Lee HJ,Lee JS,Paik YK

更新日期：2006-02-01 00:00:00

abstract：:The severe pulmonary form of leptospirosis (SPFL) is an especially serious and rapid disease process characterized by alveolar hemorrhage and acute respiratory failure. The outer membrane of Leptospira facilitates direct interactions with the environs and likely contains important constituents involved during infectio...

journal_title：Proteomics

authors： Nally JE,Whitelegge JP,Aguilera R,Pereira MM,Blanco DR,Lovett MA

更新日期：2005-01-01 00:00:00

abstract：:Proteomic analysis on sperm has been restricted to only a few model organisms. We present here a 2DE PAGE proteome map of sperm cells from a nonmodel organism, the marine mussel Mytilus edulis, a free-spawning marine invertebrate with external fertilization. Ninety-six protein spots showing high expression were select...

journal_title：Proteomics

authors： Diz AP,Dudley E,Skibinski DO

更新日期：2012-06-01 00:00:00

abstract：:Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...

journal_title：Proteomics

authors： Westers L,Westers H,Zanen G,Antelmann H,Hecker M,Noone D,Devine KM,van Dijl JM,Quax WJ

更新日期：2008-07-01 00:00:00

abstract：:Integral membrane proteins (IMPs) perform crucial cellular functions and are the primary targets for most pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains and their intimate association with lipids make them difficult to handle. Numerous proteomic platforms that include LC sepa...

journal_title：Proteomics

authors： Blackler AR,Speers AE,Wu CC

更新日期：2008-10-01 00:00:00

abstract：:Cancer research is striving toward new frontiers of assigning the correct personalized drug(s) to a given patient. However, extensive tumor heterogeneity poses a major obstacle. Tumors of the same type often respond differently to therapy, due to patient-specific molecular aberrations and/or untargeted tumor subpopula...

journal_title：Proteomics

authors： Kravchenko-Balasha N

更新日期：2020-07-01 00:00:00

abstract：:Many proteomic studies focus on quantitative aspects, using different stable isotope labeling techniques that require specialized software to analyze the generated data. Here we present jTraqX, an easy-to-use tool for processing and visualizing protein quantification data. jTraqX is platform independent and is compati...

journal_title：Proteomics

authors： Muth T,Keller D,Puetz SM,Martens L,Sickmann A,Boehm AM

更新日期：2010-03-01 00:00:00

abstract：:LTQ Orbitrap data analyzed with ProteinPilot can be further improved by MaxQuant raw data processing, which utilizes precursor-level high mass accuracy data for peak processing and MGF creation. In particular, ProteinPilot results from MaxQuant-processed peaklists for Orbitrap data sets resulted in improved spectral u...

journal_title：Proteomics

authors： Jagtap P,Bandhakavi S,Higgins L,McGowan T,Sa R,Stone MD,Chilton J,Arriaga EA,Seymour SL,Griffin TJ

更新日期：2012-06-01 00:00:00

abstract：:Despite increasing evidence of behavioural manipulation of their vectors by pathogens, the underlying mechanisms causing infected vectors to act in ways that benefit pathogen transmission remain enigmatic in most cases. Here, 2-D DIGE coupled with MS were employed to analyse and compare the head proteome of mosquitoes...

journal_title：Proteomics

authors： Lefevre T,Thomas F,Schwartz A,Levashina E,Blandin S,Brizard JP,Le Bourligu L,Demettre E,Renaud F,Biron DG

更新日期：2007-06-01 00:00:00

abstract：:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...

journal_title：Proteomics

authors： Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

更新日期：2006-12-01 00:00:00

abstract：:In the present study, changes in protein synthesis patterns after salt shock visualized by 35S-methionine labeling and the changed protein composition in salt-acclimated cells of the cyanobacterium Synechocystis sp. strain PCC 6803 were analyzed by a combination of 2-DE for protein separation and PMF for protein ident...

journal_title：Proteomics

authors： Fulda S,Mikkat S,Huang F,Huckauf J,Marin K,Norling B,Hagemann M

更新日期：2006-05-01 00:00:00

abstract：:A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differenti...

journal_title：Proteomics

authors： Winter D,Seidler J,Kugelstadt D,Derrer B,Kappes B,Lehmann WD

更新日期：2010-04-01 00:00:00

abstract：:The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...

journal_title：Proteomics

authors： Yamamoto T,Langham RG,Ronco P,Knepper MA,Thongboonkerd V

更新日期：2008-06-01 00:00:00

abstract：:Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...

journal_title：Proteomics

authors： Hare NJ,Chan B,Chan E,Kaufman KL,Britton WJ,Saunders BM

更新日期：2015-09-01 00:00:00

abstract：:Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...

journal_title：Proteomics

authors： Ebhardt HA,Sabidó E,Hüttenhain R,Collins B,Aebersold R

更新日期：2012-04-01 00:00:00

abstract：:Mitochondria consist of four compartments, outer membrane, intermembrane space, inner membrane, and matrix; each harboring specific functions and structures. In this study, we used LC-MS/MS to characterize the protein composition of Trypanosoma brucei mitochondrial (mt) membranes, which were enriched by different bioc...

journal_title：Proteomics

authors： Acestor N,Panigrahi AK,Ogata Y,Anupama A,Stuart KD

更新日期：2009-12-01 00:00:00

abstract：:As mass-spectrometry-based proteomics has matured during the past decade, a growing emphasis has been placed on quality control. For this purpose, multiple computational quality control tools have been introduced. These tools generate a set of metrics that can be used to assess the quality of a mass spectrometry exper...

journal_title：Proteomics

authors： Bittremieux W,Valkenborg D,Martens L,Laukens K

更新日期：2017-02-01 00:00:00

abstract：:The gut microbiome has been shown to play a significant role in human healthy and diseased states. The dynamic signaling that occurs between the host and microbiome is critical for the maintenance of host homeostasis. Analyzing the human microbiome with metaproteomics, metabolomics, and integrative multi-omics analyse...

journal_title：Proteomics

authors： Peters DL,Wang W,Zhang X,Ning Z,Mayne J,Figeys D

更新日期：2019-08-01 00:00:00

abstract：:Omic technologies have enabled the complete readout of the molecular state of a cell at different biological scales. In principle, the combination of multiple omic data types can provide an integrated view of the entire biological system. This integration requires appropriate models in a systems biology approach. Here...

journal_title：Proteomics

authors： Dahal S,Yurkovich JT,Xu H,Palsson BO,Yang L

更新日期：2020-09-01 00:00:00

abstract：:Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...

journal_title：Proteomics

authors： Beaudette P,Popp O,Dittmar G

更新日期：2016-01-01 00:00:00