Abstract:
:FBXO25 is one of the 68 human F-box proteins that serve as specificity factors for a family of ubiquitin ligases composed of s-phase-kinase associated protein 1, really interesting new gene-box 1, Cullin 1, and F-box protein (SCF1) that are involved in targeting proteins for destruction across the ubiquitin proteasome system. We recently reported that the FBXO25 protein accumulates in novel subnuclear structures named FBXO25-associated nuclear domains (FAND). Combining two-step affinity purification followed by MS with a classical two-hybrid screen, we identified 132 novel potential FBXO25 interacting partners. One of the identified proteins, beta-actin, physically interacts through its N-terminus with FBXO25 and is enriched in the FBXO25 nuclear compartments. Inhibitors of actin polymerization promote a significant disruption of FAND, indicating that they are compartments influenced by the organizational state of actin in the nucleus. Furthermore, FBXO25 antibodies interfered with RNA polymerase II transcription in vitro. Our results open new perspectives for the understanding of this novel compartment and its nuclear functions.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Teixeira FR,Yokoo S,Gartner CA,Manfiolli AO,Baqui MM,Assmann EM,Maragno AL,Yu H,de Lanerolle P,Kobarg J,Gygi SP,Gomes MDdoi
10.1002/pmic.200900419subject
Has Abstractpub_date
2010-08-01 00:00:00pages
2746-57issue
15eissn
1615-9853issn
1615-9861journal_volume
10pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera g...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300678
更新日期:2004-04-01 00:00:00
abstract::Interleukin (IL)-6 is a circulatory, pleiotropic cytokine with multiple roles in the immune system. Both IL-6 and the IL6 -174G>C promoter polymorphism have been linked to various diseases associated with inflammation. However, the mechanism by which the polymorphism influences disease risk is unclear. We postulated t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600366
更新日期:2007-02-01 00:00:00
abstract::Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500129
更新日期:2016-02-01 00:00:00
abstract::Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to allev...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200494
更新日期:2013-07-01 00:00:00
abstract::Recombinant human growth hormone (rhGH) is used for the treatment of several disorders. Structural integrity of rhGH is of critical importance for its clinical use and modifications thereof may act as markers in situations such as rhGH doping, as illegal rhGH-abuse in sports is of increasing interest. In the current s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500334
更新日期:2006-02-01 00:00:00
abstract::Cancer research is striving toward new frontiers of assigning the correct personalized drug(s) to a given patient. However, extensive tumor heterogeneity poses a major obstacle. Tumors of the same type often respond differently to therapy, due to patient-specific molecular aberrations and/or untargeted tumor subpopula...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900227
更新日期:2020-07-01 00:00:00
abstract::The useful structural features of class II MHC molecules are rarely integrated into T-cell epitope predictions. We propose an approach that applies a novel expectation-maximization algorithm to align the naturally processed peptides selected by the class II MHC I-A(g7) molecule - focusing on the five MHC-specific anch...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600584
更新日期:2007-02-01 00:00:00
abstract::Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401055
更新日期:2005-04-01 00:00:00
abstract::Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200208)2:8<947::AID-PROT947>3.0.
更新日期:2002-08-01 00:00:00
abstract::In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700260
更新日期:2018-04-01 00:00:00
abstract::Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potent...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900479
更新日期:2010-02-01 00:00:00
abstract::In proteomic research, two-dimensional electrophoresis (2-D) is an important tool for investigating differential patterns of qualitative and quantitative protein expression. The strength of the technique is due to its unrivalled power of being able to separate simultaneously thousands of proteins. The key to the compa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200107)1:7<856::AID-PROT856>3.0.
更新日期:2001-07-01 00:00:00
abstract::Two new iodoacetamide-substituted cyanines, C3NIASO3 and C5NIASO3, were synthesized starting from hemicyanine and were utilized for labeling plasma proteins. Specificity, sensitivity and feasibility for SH residues was tested utilizing an equimolar mixture of standard proteins and with normal plasma. Oxidized plasma p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800254
更新日期:2009-01-01 00:00:00
abstract::The large interactive surfaces of nanoparticles (NPs) increase the opportunities to develop NPs for vascular targeting. Proteomic analysis of endothelial cells exposed to NPs reveals the cellular response and turns the focus into the impairment of the endothelial permeability. Here, quantitative proteomics and transcr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800228
更新日期:2019-03-01 00:00:00
abstract::Most MS-based glycomic and glycoproteomic analyses focus on identifying changes in terminal glyco-epitopes represented by sialylation and fucosylation at specific positions of the terminal N-acetyllactosamine units. Much less attention was accorded to the underlying linear or branched poly-N-acetyllactosamine extensio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000794
更新日期:2011-07-01 00:00:00
abstract::Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenes...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000460
更新日期:2011-06-01 00:00:00
abstract::Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600513
更新日期:2007-01-01 00:00:00
abstract::Previous studies have found that some first onset schizophrenia patients show signs of impaired insulin signaling. Also, epidemiological studies have shown that periods of suboptimal nutrition including protein deficiencies during pregnancy can lead to increased incidence of metabolic conditions and psychiatric disord...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200376
更新日期:2012-12-01 00:00:00
abstract::Entamoeba histolytica is known for its extraordinary capacity to destroy human tissues, leading to invasive diseases such as ulcerative colitis or extra-intestinal abscesses. In order to identify the virulence factors of this parasite phenotypes and proteomes of two recently identified genetically related cell lines (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900022
更新日期:2009-09-01 00:00:00
abstract::New insights on cellular heterogeneity in the last decade provoke the development of a variety of single cell omics tools at a lightning pace. The resultant high-dimensional single cell data generated by these tools require new theoretical approaches and analytical algorithms for effective visualization and interpreta...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201600267
更新日期:2017-02-01 00:00:00
abstract::In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600187
更新日期:2016-11-01 00:00:00
abstract::Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300465
更新日期:2003-10-01 00:00:00
abstract::Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a 2-DE-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800636
更新日期:2009-03-01 00:00:00
abstract::High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402086
更新日期:2006-03-01 00:00:00
abstract::More than 300 different types of protein post-translational modifications (PTMs) have been described, many of which are known to have pivotal roles in cellular physiology and disease. Nevertheless, only a handful of PTMs have been extensively investigated at the proteome level. Knowledge of protein substrates and thei...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200900398
更新日期:2009-10-01 00:00:00
abstract::Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400835
更新日期:2004-10-01 00:00:00
abstract::Mass spectrometry-based proteomics is used to gain insight into the abundance and subcellular localization of cellular signaling components, the composition of molecular complexes and the regulation of signaling pathways. Multicellular organisms have evolved signaling networks and fast responses to stimuli that can be...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000418
更新日期:2011-02-01 00:00:00
abstract::MALDI-MS is an extremely flexible technique and can be synergistically used in conjunction with established bioanalytical methods such as PAGE or SPR. To that end, slight modifications on the sample target plate may be necessary. Those can involve the use of hydrophobic coatings for improved sample deposition and desa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700782
更新日期:2008-02-01 00:00:00
abstract::High-density lipoprotein (HDL) is the most abundant lipoprotein particle in the plasma and a negative risk factor of atherosclerosis. By using a proteomic approach it is possible to obtain detailed information about its protein content and protein modifications that may give new information about the physiological rol...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401010
更新日期:2005-04-01 00:00:00
abstract::There is ample evidence for the involvement of protein phosphorylation on serine/threonine/tyrosine in bacterial signaling and regulation, but very few exact phosphorylation sites have been experimentally determined. Recently, gel-free high accuracy MS studies reported over 150 phosphorylation sites in two bacterial m...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800285
更新日期:2009-01-01 00:00:00