Abstract:
:Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A431/Pt, were used as a model system. The experimental set-up involved not just a two-way comparison of the control vs. the drug-resistant cell line, but also an acute cisplatin treatment of both cell lines, leading to a four-way comparison, as follows: 1) A431 vs. A431/Pt cells; 2) A431 vs. A431 cisplatin exposed cells; 3) A431/Pt vs. A431/Pt cisplatin exposed cells; 4) A431 cisplatin exposed cells vs. A431/Pt cisplatin exposed cells. We found modulation of proteins, which could be classified under various categories, such as molecular chaperones (e.g. heat-shock proteins HSP60, HSP90, HSC71, heat-shock cognate 71 kDa protein), Ca2+-binding proteins (e.g. calmodulin, calumenin), proteins involved in drug detoxification (such as peroxiredoxins PRX 2 and PRX 6, and glutathione-S-transferase, GST), anti-apoptotic proteins (such as 14-3-3 switched on in cisplatin-exposed cells) and ion channels (such as VDAC-1, voltage-dependent anion-selective channel). In particular, the basal levels of HSC71 and HSP60 were increased in A431/Pt cells as compared to A431 cells, and cisplatin exposure resulted in up-regulation of HSP60 and HSP90 only in A431 cells. Moreover, cisplatin exposure up-regulated the anti-apoptotic 14-3-3 protein in both cell lines, GST in sensitive cells and PRX6 in A431/Pt cells. These findings are consistent with a constitutive expression of defence factors by resistant cells and with activation by cisplatin of mechanisms acting to protect cells from drug-induced damage. This pattern of response, also observed in parental cells, could reflect an intrinsic resistance of this tumor type.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Castagna A,Antonioli P,Astner H,Hamdan M,Righetti SC,Perego P,Zunino F,Righetti PGdoi
10.1002/pmic.200400835subject
Has Abstractpub_date
2004-10-01 00:00:00pages
3246-67issue
10eissn
1615-9853issn
1615-9861journal_volume
4pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Adiponectin is a fat cell-secreted hormone with antidiabetic and anti-inflammatory activities. The reduced adiponectin levels are associated with obesity-related metabolic syndrome. Replenishment of this hormone into animal models can improve insulin sensitivity, decrease blood glucose and lipid levels, and prevent th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500840
更新日期:2006-07-01 00:00:00
abstract::Voltage-dependent anion channel (VDAC) proteins are small, abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apopt...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300734
更新日期:2004-05-01 00:00:00
abstract::Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600513
更新日期:2007-01-01 00:00:00
abstract::Staphylococcus aureus Clp ATPases (molecular chaperones) alter normal physiological functions including an aconitase-mediated effect on post-stationary growth, acetate catabolism, and entry into death phase (Chatterjee et al., J. Bacteriol. 2005, 187, 4488-4496). In the present study, the global function of ClpC in ph...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800586
更新日期:2009-03-01 00:00:00
abstract::Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000316
更新日期:2010-11-01 00:00:00
abstract::Somatic embryogenesis is a biotechnological approach mainly used for the clonal propagation of different plants worldwide. In somatic embryogenesis, embryos arise from somatic cells under appropriate culture conditions. This plasticity in plants is a demonstration of true cellular totipotency and is the best approach ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201700265
更新日期:2018-03-01 00:00:00
abstract::As we transition from genomics to the challenges of the functional proteome, new tools to explore the expression of proteins within tissue are essential. We have developed a method of transferring proteins from a formalin fixed, paraffin embedded tissues section to a stack of membranes which is then probed with antibo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401343
更新日期:2006-02-01 00:00:00
abstract::Proteins extracted from leaf blades of rice plants infected with blast fungus, Magnaporthe grisea, were separated by two-dimensional polyacrylamide gel electrophoresis. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane, and 63 proteins were analyzed by a gas-phase protein sequencer. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200109)1:9<1162::AID-PROT1162>3.
更新日期:2001-09-01 00:00:00
abstract::Menthol is a naturally occurring cyclic monoterpene used in oral hygiene products, confectionary, pharmaceuticals, cosmetics, pesticides, and as a flavoring agent. In the present study, we analyzed the differentially expressing proteome in L-menthol-treated Caco-2 cell line as it was found to inhibit cell proliferatio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000691
更新日期:2011-05-01 00:00:00
abstract::By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300488
更新日期:2003-08-01 00:00:00
abstract::Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100186
更新日期:2011-10-01 00:00:00
abstract::Imbalance in protein homeostasis in specific subcellular organelles is alleviated through organelle-specific stress response pathways. As a canonical example of stress activated pathway, accumulation of misfolded proteins in ER activates unfolded protein response (UPR) in almost all eukaryotic organisms. However, very...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300449
更新日期:2014-08-01 00:00:00
abstract::We here present a user-friendly and extremely lightweight tool that can serve as a stand-alone front-end for the Open MS Search Algorithm (OMSSA) search engine, or that can directly be used as part of an informatics processing pipeline for MS driven proteomics. The OMSSA graphical user interface (OMSSAGUI) tool is wri...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701126
更新日期:2008-06-01 00:00:00
abstract::Plant tissues contain large amounts of secondary compounds that significantly interfere with protein extraction and 2DE analysis. Thus, sample preparation is a crucial step prior to 2DE in plant proteomics. This tutorial highlights the guidelines that need to be followed to perform an adequate total protein extraction...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300239
更新日期:2014-03-01 00:00:00
abstract::Realistic in vitro models are critical in the drug development process. In this study, a novel in vitro platform is employed to assess drug penetration and metabolism. This platform, which utilizes a 3D printed fluidic device, allows for dynamic dosing of three dimensional cell cultures, also known as spheroids. The p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500524
更新日期:2016-06-01 00:00:00
abstract::Two-dimensional difference gel electrophoresis (2-D DIGE) allows for reliable quantification of global protein abundance changes. The threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). This study estimates biological, technical and total variation ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100071
更新日期:2011-08-01 00:00:00
abstract::In shotgun proteomics, proteins can be fractionated by 1-D gel electrophoresis and digested into peptides, followed by liquid chromatography to separate the peptide mixture. Mass spectrometry generates hundreds of thousands of tandem mass spectra from these fractions, and proteins are identified by database searching....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500318
更新日期:2006-02-01 00:00:00
abstract::Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a 2-DE-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800636
更新日期:2009-03-01 00:00:00
abstract::SARS-CoV-2 infection results in a mild-to-moderate disease course in most patients, allowing outpatient self-care and quarantine. However, in approx. 10% of cases a two- or three-phasic critical disease course with starting from day 7 to 10 is observed. To facilitate and plan outpatient care, biomarkers prognosing suc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000202
更新日期:2020-09-10 00:00:00
abstract::Most cancers consist of heterogeneous populations of cells with substantial differences in tumorigenicity. Cells that possess self-renewal and tumor-initiating properties are often called cancer stem cells (CSCs). Since CSCs underlie tumor recurrence and metastasis and are resistant to current anti-cancer therapies, n...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201500472
更新日期:2016-12-01 00:00:00
abstract::Interleukin (IL)-6 is a circulatory, pleiotropic cytokine with multiple roles in the immune system. Both IL-6 and the IL6 -174G>C promoter polymorphism have been linked to various diseases associated with inflammation. However, the mechanism by which the polymorphism influences disease risk is unclear. We postulated t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600366
更新日期:2007-02-01 00:00:00
abstract::To better understand the underlying molecular basis of leaf development in maize, a reference map of nuclear proteins in basal region of seedling leaf was established using a combination of 2DE and MALDI-TOF-MS. In total, 441 reproducible protein spots in nuclear proteome of maize leaf basal region were detected with ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300147
更新日期:2014-05-01 00:00:00
abstract::Lipid particles (LP) of all types of cells are a depot of neutral lipids. The present investigation deals with the isolation of LP from the yeast Yarrowia lipolytica and the characterization of their lipid and protein composition. Properties of LP varied depending on the carbon source. LP from glucose-grown cells reve...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500339
更新日期:2006-03-01 00:00:00
abstract::A large repertoire of immunological methods permits monitoring the interaction of antibodies with their specific antigen. However, recognition of a protein by a conformation-specific antibody represents a challenge because native conditions must be kept throughout the assay. Native immunoblotting of blue native gels c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900468
更新日期:2010-01-01 00:00:00
abstract::Of all protein PTMs, glycosylation is by far the most common, and is a target for proteomic research. Glycosylation plays key roles in controlling various cellular processes and the modifications of the glycan structures in diseases highlight the clinical importance of this PTM. Glycosylation analysis remains a diffic...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600129
更新日期:2006-07-01 00:00:00
abstract::Staphylococcus aureus is an opportunistic human pathogen, which can cause life-threatening disease. Proteome analyses of the bacterium can provide new insights into its pathophysiology and important facets of metabolic adaptation and, thus, aid the recognition of targets for intervention. However, the value of such pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500091
更新日期:2015-11-01 00:00:00
abstract::We report on quality control performed in the context of a large-scale, multi-institutional study of the immune response in blood samples from prostate cancer patients. The measurements were performed by two commercially available techniques/services: protein arrays and an automated bead-based ELISA-like technique on ...
journal_title:Proteomics
pub_type: 杂志文章,多中心研究
doi:10.1002/pmic.201200082
更新日期:2012-09-01 00:00:00
abstract::This study aimed at establishing a sensitive multiple reaction monitoring-mass spectrometry (MRM-MS) method for the quantification of the drug metabolizing cytochrome P450 (CYP)3A4 enzyme in human liver homogenates. Liver samples were subjected to trypsin digestion. MRM-MS analyses were performed using three transitio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500386
更新日期:2016-11-01 00:00:00
abstract::The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900836
更新日期:2010-05-01 00:00:00
abstract::In this work, for the first time, a novel C60-functionalized magnetic silica microsphere (designated C60-f-MS) was synthesized by radical polymerization of C60 molecules on the surface of magnetic silica microspheres. The resulting C60-f-MS microsphere has magnetite core and thin C60 modified silica shell, which endow...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800335
更新日期:2009-01-01 00:00:00