A proteomic approach to cisplatin resistance in the cervix squamous cell carcinoma cell line A431.


:Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A431/Pt, were used as a model system. The experimental set-up involved not just a two-way comparison of the control vs. the drug-resistant cell line, but also an acute cisplatin treatment of both cell lines, leading to a four-way comparison, as follows: 1) A431 vs. A431/Pt cells; 2) A431 vs. A431 cisplatin exposed cells; 3) A431/Pt vs. A431/Pt cisplatin exposed cells; 4) A431 cisplatin exposed cells vs. A431/Pt cisplatin exposed cells. We found modulation of proteins, which could be classified under various categories, such as molecular chaperones (e.g. heat-shock proteins HSP60, HSP90, HSC71, heat-shock cognate 71 kDa protein), Ca2+-binding proteins (e.g. calmodulin, calumenin), proteins involved in drug detoxification (such as peroxiredoxins PRX 2 and PRX 6, and glutathione-S-transferase, GST), anti-apoptotic proteins (such as 14-3-3 switched on in cisplatin-exposed cells) and ion channels (such as VDAC-1, voltage-dependent anion-selective channel). In particular, the basal levels of HSC71 and HSP60 were increased in A431/Pt cells as compared to A431 cells, and cisplatin exposure resulted in up-regulation of HSP60 and HSP90 only in A431 cells. Moreover, cisplatin exposure up-regulated the anti-apoptotic 14-3-3 protein in both cell lines, GST in sensitive cells and PRX6 in A431/Pt cells. These findings are consistent with a constitutive expression of defence factors by resistant cells and with activation by cisplatin of mechanisms acting to protect cells from drug-induced damage. This pattern of response, also observed in parental cells, could reflect an intrinsic resistance of this tumor type.






Castagna A,Antonioli P,Astner H,Hamdan M,Righetti SC,Perego P,Zunino F,Righetti PG




Has Abstract


2004-10-01 00:00:00












  • Protein expression profile of Gluconacetobacter diazotrophicus PAL5, a sugarcane endophytic plant growth-promoting bacterium.

    abstract::This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MA...


    pub_type: 杂志文章


    authors: Lery LM,Coelho A,von Kruger WM,Gonçalves MS,Santos MF,Valente RH,Santos EO,Rocha SL,Perales J,Domont GB,Teixeira KR,Bertalan M,Ferreira PC,Bisch PM

    更新日期:2008-04-01 00:00:00

  • Proteomic and transcriptional characterization of aromatic degradation pathways in Rhodoccocus sp. strain TFB.

    abstract::Rhodococcus sp. strain TFB is a versatile gram-positive bacterium able to grow on a wide variety of aromatic compounds as carbon and energy sources. Since the strain is refractory to genetic analysis, a proteomic approach was used to study the metabolic pathways involved in the catabolism of such compounds by analyzin...


    pub_type: 杂志文章


    authors: Tomás-Gallardo L,Canosa I,Santero E,Camafeita E,Calvo E,López JA,Floriano B

    更新日期:2006-04-01 00:00:00

  • Tumor-Derived Extracellular Vesicles as a Novel Source of Protein Biomarkers for Cancer Diagnosis and Monitoring.

    abstract::"Liquid biopsies" have received attention as a complementary tool for traditional tissue biopsies that may enhance the spectrum of analysis for tumor-derived factors. One such factor gaining prominence in the liquid biopsy field is extracellular vesicles (EVs), membrane-bound nanovesicles which are secreted by cells i...


    pub_type: 杂志文章,评审


    authors: Ruhen O,Meehan K

    更新日期:2019-01-01 00:00:00

  • Study of rat hypothalamic proteome by HPLC/ESI ion trap and HPLC/ESI-Q-TOF MS.

    abstract::The proteomic profile of hypothalamus, a key organ of CNS, is explored here by employing two widely used MS techniques, i.e. HPLC/ESI-ion trap and HPLC/ESI-quadrupole-TOF MS. Strong cation exchange is used for the fractionation of peptides and protein search engine MASCOT is employed for data query. One hundred and th...


    pub_type: 杂志文章


    authors: Iqbal J,Li W,Ullah K,Hasan M,Linna G,Awan U,Zhang Y,Batool S,Qing H,Deng Y

    更新日期:2013-08-01 00:00:00

  • Torches, Candles, Lamps, Lanterns, Flashlights, Spotlights, Night Vision Goggles… You Need Them All to See in Darkness.

    abstract::Articles assembled in the second part of this Special Issue describe some experimental and computational approaches for the structural and functional characterization of intrinsically disordered proteins. Since these tools represent specialized gear for the focused analysis of various aspects of dark proteome, they ca...


    pub_type: 社论


    authors: Uversky VN

    更新日期:2019-03-01 00:00:00

  • Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ.

    abstract::Aging is associated with a progressive loss of skeletal muscular function that often leads to progressive disability and loss of independence. Although muscle aging is well documented, the molecular mechanisms of this condition still remain unclear. To gain greater insight into the changes associated with aging of ske...


    pub_type: 杂志文章


    authors: Hwang CY,Kim K,Choi JY,Bahn YJ,Lee SM,Kim YK,Lee C,Kwon KS

    更新日期:2014-01-01 00:00:00

  • Mass spectrometry-based immuno-precipitation proteomics - the user's guide.

    abstract::Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...


    pub_type: 杂志文章


    authors: ten Have S,Boulon S,Ahmad Y,Lamond AI

    更新日期:2011-03-01 00:00:00

  • Differential expression profile of membrane proteins in zebrafish (Danio rerio) brain exposed to methyl parathion.

    abstract::Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (...


    pub_type: 杂志文章


    authors: Huang QY,Huang HQ

    更新日期:2011-09-01 00:00:00

  • Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium.

    abstract::Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...


    pub_type: 杂志文章


    authors: Hudelist G,Singer CF,Pischinger KI,Kaserer K,Manavi M,Kubista E,Czerwenka KF

    更新日期:2006-03-01 00:00:00

  • Proteomic analysis of ligamentum flavum from patients with lumbar spinal stenosis.

    abstract::Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...


    pub_type: 杂志文章


    authors: Kamita M,Mori T,Sakai Y,Ito S,Gomi M,Miyamoto Y,Harada A,Niida S,Yamada T,Watanabe K,Ono M

    更新日期:2015-05-01 00:00:00

  • Identification of specific plant nucleolar phosphoproteins in a functional proteomic analysis.

    abstract::The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...


    pub_type: 杂志文章


    authors: González-Camacho F,Medina FJ

    更新日期:2004-02-01 00:00:00

  • Shotgun proteome analysis utilising mixed mode (reversed phase-anion exchange chromatography) in conjunction with reversed phase liquid chromatography mass spectrometry analysis.

    abstract::The 2-D peptide separations employing mixed mode reversed phase anion exchange (MM (RP-AX)) HPLC in the first dimension in conjunction with RP chromatography in the second dimension were developed and utilised for shotgun proteome analysis. Compared with strong cation exchange (SCX) typically employed for shotgun prot...


    pub_type: 杂志文章


    authors: Phillips HL,Williamson JC,van Elburg KA,Snijders AP,Wright PC,Dickman MJ

    更新日期:2010-08-01 00:00:00

  • Proteins unique to intraphagosomally grown Mycobacterium tuberculosis.

    abstract::Pathogenic mycobacteria persist and replicate within phagosomes of host phagocytes by inhibiting phagosome maturation at an early endosome stage. The molecular basis for this behavior is not understood. To identify proteins of Mycobacterium tuberculosis unique to the intraphagosomal phase, mycobacteria were purified f...


    pub_type: 杂志文章


    authors: Mattow J,Siejak F,Hagens K,Becher D,Albrecht D,Krah A,Schmidt F,Jungblut PR,Kaufmann SH,Schaible UE

    更新日期:2006-04-01 00:00:00

  • Identification of tumor-associated plasma biomarkers using proteomic techniques: from mouse to human.

    abstract::In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...


    pub_type: 杂志文章


    authors: Juan HF,Chen JH,Hsu WT,Huang SC,Chen ST,Yi-Chung Lin J,Chang YW,Chiang CY,Wen LL,Chan DC,Liu YC,Chen YJ

    更新日期:2004-09-01 00:00:00

  • Identification and characterization of highly expressed proteins in sperm cells of the marine mussel Mytilus edulis.

    abstract::Proteomic analysis on sperm has been restricted to only a few model organisms. We present here a 2DE PAGE proteome map of sperm cells from a nonmodel organism, the marine mussel Mytilus edulis, a free-spawning marine invertebrate with external fertilization. Ninety-six protein spots showing high expression were select...


    pub_type: 杂志文章


    authors: Diz AP,Dudley E,Skibinski DO

    更新日期:2012-06-01 00:00:00

  • A rapid and simple procedure for the depletion of abundant storage proteins from legume seeds to advance proteome analysis: a case study using Glycine max.

    abstract::2-D analysis of plant proteomes containing thousands of proteins has limited dynamic resolution because only abundant proteins can be detected. Proteomic assessment of the non-abundant proteins within seeds is difficult when 60-80% is storage proteins. Resolution can be improved through sample fractionation using sepa...


    pub_type: 杂志文章


    authors: Krishnan HB,Oehrle NW,Natarajan SS

    更新日期:2009-06-01 00:00:00

  • A phosphoproteomic screen demonstrates differential dependence on HER3 for MAP kinase pathway activation by distinct PIK3CA mutations.

    abstract::The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...


    pub_type: 杂志文章


    authors: Blair BG,Wu X,Zahari MS,Mohseni M,Cidado J,Wong HY,Beaver JA,Cochran RL,Zabransky DJ,Croessmann S,Chu D,Toro PV,Cravero K,Pandey A,Park BH

    更新日期:2015-01-01 00:00:00

  • Partial hepatectomy induced liver proteome changes in mice.

    abstract::Acceleration of liver regeneration could be of great clinical benefit in various liver-associated diseases. However, at present little is known about therapeutic interventions to enhance this regenerative process. Our limited understanding and the complexity of the mechanisms involved have prevented the identification...


    pub_type: 杂志文章


    authors: Strey CW,Winters MS,Markiewski MM,Lambris JD

    更新日期:2005-01-01 00:00:00

  • An improved method to unravel phosphoacceptors in Ser/Thr protein kinase-phosphorylated substrates.

    abstract::Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...


    pub_type: 杂志文章


    authors: Molle V,Leiba J,Zanella-Cléon I,Becchi M,Kremer L

    更新日期:2010-11-01 00:00:00

  • Processing strategies and software solutions for data-independent acquisition in mass spectrometry.

    abstract::Data-independent acquisition (DIA) offers several advantages over data-dependent acquisition (DDA) schemes for characterizing complex protein digests analyzed by LC-MS/MS. In contrast to the sequential detection, selection, and analysis of individual ions during DDA, DIA systematically parallelizes the fragmentation o...


    pub_type: 杂志文章,评审


    authors: Bilbao A,Varesio E,Luban J,Strambio-De-Castillia C,Hopfgartner G,Müller M,Lisacek F

    更新日期:2015-03-01 00:00:00

  • Sensitive, quantitative, and fast modifications for Coomassie Blue staining of polyacrylamide gels.

    abstract::In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons: Low price, Visibl...


    pub_type: 杂志文章


    authors: Westermeier R

    更新日期:2006-09-01 00:00:00

  • Proteomics of corynebacteria: From biotechnology workhorses to pathogens.

    abstract::Corynebacteria belong to the high G+C Gram-positive bacteria (Actinobacteria) and are closely related to Mycobacterium and Nocardia species. The best investigated member of this group of almost seventy species is Corynebacterium glutamicum, a soil bacterium isolated in 1957, which is used for the industrial production...


    pub_type: 杂志文章,评审


    authors: Poetsch A,Haussmann U,Burkovski A

    更新日期:2011-08-01 00:00:00

  • The affinity of chick cofilin for actin increases when actin is complexed with DNase I: formation of a cofilin-actin-DNase I ternary complex.

    abstract::Cofilin, an actin-binding protein, regulates the rate, nature and extent of assembly of the actin cytoskeleton. Native Phast gels show that the addition of cofilin to an actin-DNase I complex (74 kDa) results in the formation of a ternary complex of 94 kDa indicating an equimolar stoichiometry in the ternary complex. ...


    pub_type: 杂志文章


    authors: Nosworthy NJ,Kekic M,dos Remedios CG

    更新日期:2001-12-01 00:00:00

  • Kinetic characterization of sequencing grade modified trypsin.

    abstract::Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...


    pub_type: 杂志文章


    authors: Finehout EJ,Cantor JR,Lee KH

    更新日期:2005-06-01 00:00:00

  • Pyruvate dehydrogenase alpha 1 as a target of omega-3 polyunsaturated fatty acids in human prostate cancer through a global phosphoproteomic analysis.

    abstract::Prostate cancer is one of the leading cancers in men. Taking dietary supplements, such as fish oil (FO), which is rich in n-3 polyunsaturated fatty acids (PUFAs), has been employed as a strategy to lower prostate cancer risk and control disease progression. In this study, we investigated the global phosphoproteomic ch...


    pub_type: 杂志文章


    authors: Zhao H,Pflug BR,Lai X,Wang M

    更新日期:2016-09-01 00:00:00

  • Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis.

    abstract::The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...


    pub_type: 杂志文章


    authors: Mehaffy C,Hess A,Prenni JE,Mathema B,Kreiswirth B,Dobos KM

    更新日期:2010-05-01 00:00:00

  • Quantitative proteomic analysis of HIV-1 Tat-induced dysregulation in SH-SY5Y neuroblastoma cells.

    abstract::Despite affecting up to 70% of HIV-positive patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on...


    pub_type: 杂志文章


    authors: Ganief T,Gqamana P,Garnett S,Hoare J,Stein DJ,Joska J,Soares N,Blackburn JM

    更新日期:2017-03-01 00:00:00

  • Strategies to improve usability and preserve accuracy in biological sequence databases.

    abstract::Biology is increasingly dependent on large-scale analysis, such as proteomics, creating a requirement for efficient bioinformatics. Bioinformatic predictions of biological functions rely upon correctly annotated database sequences, and the presence of inaccurately annotated or otherwise poorly described sequences intr...


    pub_type: 杂志文章


    authors: Bengtsson-Palme J,Boulund F,Edström R,Feizi A,Johnning A,Jonsson VA,Karlsson FH,Pal C,Pereira MB,Rehammar A,Sanchez J,Sanli K,Thorell K

    更新日期:2016-09-01 00:00:00

  • Novel identification of expressed genes and functional classification of hypothetical proteins from Neisseria meningitidis serogroup A.

    abstract::To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by microLC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene ex...


    pub_type: 杂志文章


    authors: Bernardini G,Arena S,Braconi D,Scaloni A,Santucci A

    更新日期:2007-09-01 00:00:00

  • The Equine PeptideAtlas: a resource for developing proteomics-based veterinary research.

    abstract::Progress in MS-based methods for veterinary research and diagnostics is lagging behind compared to the human research, and proteome data of domestic animals is still not well represented in open source data repositories. This is particularly true for the equine species. Here we present a first Equine PeptideAtlas enco...


    pub_type: 杂志文章


    authors: Bundgaard L,Jacobsen S,Sørensen MA,Sun Z,Deutsch EW,Moritz RL,Bendixen E

    更新日期:2014-03-01 00:00:00