Abstract:
:Voltage-dependent anion channel (VDAC) proteins are small, abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. A proteomic approach, consisting of two-dimensional gel electrophoresis followed by two-dimensional immunoblotting with anti-VDAC and anti-phosphotyrosine antibodies and by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, was exploited to define the expression pattern of VDAC isoforms in guinea pig brain synaptosomes, both in normoxic and hypoxic conditions. In this way a total of five different VDAC isoforms were identified, as both VDAC1 and VDAC2 were detected in more than one electrophoretic spot. Moreover, VDAC isoforms selectively undergo hypoxia-induced tyrosine phosphorylation, suggesting that tyrosine phosphorylation may contribute to the modulation of VDAC protein function/conformation or interaction with other proteins in hypoxic conditions.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Liberatori S,Canas B,Tani C,Bini L,Buonocore G,Godovac-Zimmermann J,Mishra OP,Delivoria-Papadopoulos M,Bracci R,Pallini Vdoi
10.1002/pmic.200300734subject
Has Abstractpub_date
2004-05-01 00:00:00pages
1335-40issue
5eissn
1615-9853issn
1615-9861journal_volume
4pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::In this study we investigated the cellular response to the application of high hydrostatic pressure. High pressure is increasingly used for food preservation. With high resolution 2-D electrophoresis we compared the protein patterns of atmospherically grown Lactobacillus sanfranciscensis with those pressure treated up...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200206)2:6<765::AID-PROT765>3.0.
更新日期:2002-06-01 00:00:00
abstract::Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701110
更新日期:2008-06-01 00:00:00
abstract::Roots, leaves, and intermediate sections between roots and leaves (ISRL) of wheat seedlings show different physiological functions at the protein level. We performed the first integrative proteomic analysis of different tissues of the drought-tolerant wheat cultivar Hanxuan 10 (HX-10) and drought-sensitive cultivar Ch...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400179
更新日期:2015-05-01 00:00:00
abstract::Immune proteomics is an increasingly powerful tool for the investigation of the adaptive immune response to natural encounters between micro-organisms and their hosts. The versatile species Staphylococcus aureus serves to illustrate how these techniques can be employed to appreciate the complexity and diversity of the...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100010
更新日期:2011-08-01 00:00:00
abstract::Gan et al. (Proteomics 2013, 13, 3117-3123) described a new "macropore" protocol for effective protein digestion by trypsin suitable for a wide range of pH including acidic pH. It was effective not only in experiments with solutions of a model protein (myoglobin), but also with a subfraction of rat liver cytosol. This...
journal_title:Proteomics
pub_type: 评论,杂志文章
doi:10.1002/pmic.201300440
更新日期:2013-11-01 00:00:00
abstract::Phosphoproteomics can be defined as the comprehensive study of protein phosphorylation by identification of the phosphoproteins, exact mapping of the phosphorylation sites, quantification of phosphorylation, and eventually, revealing their biological function. Its place in today's research is vitally important to addr...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600232
更新日期:2006-10-01 00:00:00
abstract::This review provides a brief overview of the development of data-independent acquisition (DIA) mass spectrometry-based proteomics and selected DIA data analysis tools. Various DIA acquisition schemes for proteomics are summarized first including Shotgun-CID, DIA, MSE , PAcIFIC, AIF, SWATH, MSX, SONAR, WiSIM, BoxCar, S...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201900276
更新日期:2020-09-01 00:00:00
abstract::Blood plasma and serum are very useful samples for the detection, identification and quantitation of proteins associated with both health and disease. However, analysis of plasma and serum is a challenge because traces of interesting polypeptides and proteins can be dominated by the very high concentration of albumin ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401065
更新日期:2005-03-01 00:00:00
abstract::The protein components of urine are useful indicators of renal function and human health in general. Urine samples are easily attainable making them ideal substrates for biomarker research. Analysis of the urine proteome however, has been hindered by the great variability of the urine specimens, and the presence of va...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500671
更新日期:2006-08-01 00:00:00
abstract::Site-specific isomerization of uridines into pseudouridines in RNAs is catalyzed either by stand-alone enzymes or by box H/ACA ribonucleoprotein particles (sno/sRNPs). The archaeal box H/ACA sRNPs are five-component complexes that consist of a guide RNA and the aCBF5, aNOP10, L7Ae, and aGAR1 proteins. In this study, w...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400529
更新日期:2015-08-01 00:00:00
abstract::The comprehension of sexually different responses in dioecious plants to excess manganese (Mn) stress requires molecular explanation. Physiological and proteomic changes in leaves of Populus cathayana males and females were analyzed after 4 wk of exposure to Mn stress. Under excess Mn conditions, shoot height and phot...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200365
更新日期:2013-08-01 00:00:00
abstract::Plants exhibit resistance against incompatible pathogens, via localized and systemic responses as part of an integrated defense mechanism. To study the compatible and incompatible interactions between rice and bacteria, a proteomic approach was applied. Rice cv. Java 14 seedlings were inoculated with compatible (Xo743...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600470
更新日期:2006-11-01 00:00:00
abstract::CREC proteins constitute a family of EF-hand calcium binding proteins localized to the secretory pathway. Calumenin is the only member known to be secreted. Recently, it was shown that thrombin-activated thrombocytes liberate calumenin, which also is found in atherosclerotic lesions but not in normal vasculature. To s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500686
更新日期:2006-06-01 00:00:00
abstract::In an attempt to improve the detection of peroxisome proliferation as a biomarker in environmental pollution assessment, we have applied a novel approach based on peroxisomal proteomics. Peroxisomal proteins from digestive glands of mussels Mytilus galloprovincialis were analyzed using 2-DE and MS. We have generated a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401243
更新日期:2005-10-01 00:00:00
abstract::Aberrant telomerase reactivation in differentiated cells represents a major event in oncogenic transformation. Recurrent somatic mutations in the human telomerase reverse transcriptase (TERT) promoter region, predominantly localized to two nucleotide positions, are highly prevalent in many cancer types. Both mutations...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500327
更新日期:2016-02-01 00:00:00
abstract::One of the major challenges facing protein analysis is the dynamic range of protein expression within massively complex samples (Corthals, G. L. et al.., Electrophoresis 2000, 21, 1104-1115). In plasma this difference is as great as ten orders of magnitude, and this is currently beyond the range of detection achievabl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401160
更新日期:2005-08-01 00:00:00
abstract::Since the identification of approximately 25,000 proteins from the draft human genome assembly in 2001, estimates of the total have oscillated between 30,000 and 70,000. The recently announced genome closure has not generated a consensus gene count despite this being a key parameter for many areas of biology including...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200300700
更新日期:2004-06-01 00:00:00
abstract::Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800469
更新日期:2019-06-01 00:00:00
abstract::Comparative proteome profiling using stable isotope peptide labelling and mass spectrometry has emerged as a promising strategy. Here, we show the broad potential of our proprietary protein sequence tag (PST) technology. A special feature of PST is its ability to detect a wide variety of proteins including the pharmac...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401306
更新日期:2005-06-01 00:00:00
abstract::The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900836
更新日期:2010-05-01 00:00:00
abstract::As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600722
更新日期:2007-04-01 00:00:00
abstract::To exploit advances in proteomics for drug discovery, high-throughout methods for target validation that directly address the cellular roles of proteins are required. To do this, we have characterized fluorophore-assisted light inactivation (FALI) which uses coherent or diffuse light targeted by fluorescein-labeled pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200203)2:3<247::aid-prot247>3.0.
更新日期:2002-03-01 00:00:00
abstract::Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, inclu...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700556
更新日期:2008-04-01 00:00:00
abstract::Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500558
更新日期:2006-04-01 00:00:00
abstract::Infection with the human liver fluke, Opisthorchis viverrini, is a serious public health problem in Thailand, Laos and nearby locations in Southeast Asia. Both experimental and epidemiological evidence strongly implicate liver fluke infection in the etiology of one of the liver cancer subtypes, cholangiocarcinoma (CCA...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900393
更新日期:2010-03-01 00:00:00
abstract::The main goal of this study was to analyze, using proteomic techniques, changes in protein expression of acute myeloid leukemia (AML) cells that could give insights into a better early prognosis for tumor pathophysiology. Proteomic analysis of different subtypes of AML cells was carried out using 2-DE and MALDI-TOF PM...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500384
更新日期:2006-04-01 00:00:00
abstract::The thermophilic bacterium Bacillus stearothermophilus P1 is unique in its ability to thrive in extreme environments such as high temperatures or high pH conditions. The study of cold shock response is very interesting and interpreted as a shock response to express the genes involved in synthesis of specific proteins....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200209)2:9<1316::AID-PROT1316>3.
更新日期:2002-09-01 00:00:00
abstract::Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300031
更新日期:2013-11-01 00:00:00
abstract::Rhodococcus sp. strain TFB is a versatile gram-positive bacterium able to grow on a wide variety of aromatic compounds as carbon and energy sources. Since the strain is refractory to genetic analysis, a proteomic approach was used to study the metabolic pathways involved in the catabolism of such compounds by analyzin...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500422
更新日期:2006-04-01 00:00:00
abstract::Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800009
更新日期:2008-07-01 00:00:00