Combining native MS approaches to decipher archaeal box H/ACA ribonucleoprotein particle structure and activity.

abstract：:Calcineurin is a heterodimeric serine/threonine protein phosphatase, important for many cellular processes such as T-cell regulation, cardiac hypertrophy and kidney development. We previously reported the characterization of Caenorhabditis elegans calcineurin mutants as providing a simple but excellent genetic model s...

journal_title：Proteomics

authors： Ahn DH,Singaravelu G,Lee S,Ahnn J,Shim YH

更新日期：2006-02-01 00:00:00

abstract：:Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...

journal_title：Proteomics

authors： Hudelist G,Singer CF,Pischinger KI,Kaserer K,Manavi M,Kubista E,Czerwenka KF

更新日期：2006-03-01 00:00:00

abstract：:Targeted quantitative proteomic analyses aim at systematically measuring the abundance of proteins in large sets of samples, without biases or missing values. One typical implementation is the verification of biomarker candidates in bodily fluids, which measures extended lists of validated transitions using triple qua...

journal_title：Proteomics

authors： Lesur A,Domon B

更新日期：2015-03-01 00:00:00

abstract：:Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multi...

journal_title：Proteomics

authors： Moretti M,Grunau A,Minerdi D,Gehrig P,Roschitzki B,Eberl L,Garibaldi A,Gullino ML,Riedel K

更新日期：2010-09-01 00:00:00

abstract：:During clinical mastitis in dairy cows, the quantity of milk produced decreases and the composition of the milk is altered. As the severity of inflammation associated with the disease increases, the chemical composition of milk approaches that of blood as a consequence of increased permeability of the blood mammary ba...

journal_title：Proteomics

authors： Hogarth CJ,Fitzpatrick JL,Nolan AM,Young FJ,Pitt A,Eckersall PD

更新日期：2004-07-01 00:00:00

abstract：:MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...

journal_title：Proteomics

authors： Helsens K,Martens L,Vandekerckhove J,Gevaert K

更新日期：2007-02-01 00:00:00

abstract：:Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are an important class of glycoproteins that are tethered to the surface of mammalian cells via the lipid GPI. GPI-APs have been implicated in many important cellular functions including cell adhesion, cell signaling, and immune regulation. Proteomic identificat...

journal_title：Proteomics

authors： Cortes LK,Vainauskas S,Dai N,McClung CM,Shah M,Benner JS,Corrêa IR Jr,VerBerkmoes NC,Taron CH

更新日期：2014-11-01 00:00:00

abstract：:Human plasma is the most clinically valuable specimen, containing not only a dynamic concentration range of protein components, but also several groups of high-abundance proteins that seriously interfere with the detection of low-abundance potential biomarker proteins. To establish a high-throughput method for efficie...

journal_title：Proteomics

authors： Cho SY,Lee EY,Lee JS,Kim HY,Park JM,Kwon MS,Park YK,Lee HJ,Kang MJ,Kim JY,Yoo JS,Park SJ,Cho JW,Kim HS,Paik YK

更新日期：2005-08-01 00:00:00

abstract：:Proteins determine the quality of barley in malting and brewing end-uses. In this regard, water-soluble barley proteins play a major role in the formation, stability, and texture of head foams. Our objective was to survey the barley seed proteins that could be involved in the foaming properties of beer. Therefore, two...

journal_title：Proteomics

authors： Perrocheau L,Rogniaux H,Boivin P,Marion D

更新日期：2005-07-01 00:00:00

abstract：:The availability of completed genome sequences of several eukaryotic and prokaryotic species has shifted the focus towards the identification and characterization of all gene products that are expressed in a given organism. In order to cope with the huge amounts of data that have been provided by large-scale sequencin...

journal_title：Proteomics

authors： Auerbach D,Thaminy S,Hottiger MO,Stagljar I

更新日期：2002-06-01 00:00:00

abstract：:CRISPR-Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non-histone chromosomal protein HMG-14 (HMGN1) in regulating chromatin structure and tumor immunity, gene knockout of HMGN1 is performed by CRISPR in cancer cells and the...

journal_title：Proteomics

authors： Mehnert M,Li W,Wu C,Salovska B,Liu Y

更新日期：2019-07-01 00:00:00

abstract：:We present here an effective technique for the large-scale separation and identification of N-linked glycoproteins from Chang liver cells, the human normal liver cells. To enrich N-linked glycoproteins from the whole cells, a procedure containing the lysis of human liver cells, the solubilization of total proteins, le...

journal_title：Proteomics

authors： Xu Z,Zhou X,Lu H,Wu N,Zhao H,Zhang L,Zhang W,Liang YL,Wang L,Liu Y,Yang P,Zha X

更新日期：2007-07-01 00:00:00

abstract：:The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...

journal_title：Proteomics

authors： Odom GJ,Ban Y,Colaprico A,Liu L,Silva TC,Sun X,Pico AR,Zhang B,Wang L,Chen X

更新日期：2020-11-01 00:00:00

abstract：:Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due ...

journal_title：Proteomics

authors： Yuan C,Jiao L,Yang L,Ying W,Hu Z,Liu J,Cui F,Li L,Qian L,Teng Y,Hang H,Qian X,Yang X

更新日期：2008-06-01 00:00:00

abstract：:Cnidarians are widely distributed basal metazoans that play an important role in the marine ecosystem. Their genetic diversity and dispersal depends on successful oogenesis, fertilization and embryogenesis. To understand the processes that lead to successful embryogenesis in these basal organisms, we conducted compara...

journal_title：Proteomics

authors： Levitan S,Sher N,Brekhman V,Ziv T,Lubzens E,Lotan T

更新日期：2015-12-01 00:00:00

abstract：:High-affinity molecular pairs provide a convenient and flexible modular base for the design of molecular probes and protein/antigen assays. Specificity and sensitivity performance indicators of a bioassay critically depend on the dissociation constant (K(D)) of the molecular pair, with avidin:biotin being the state-of...

journal_title：Proteomics

authors： Sreenivasan VK,Kelf TA,Grebenik EA,Stremovskiy OA,Say JM,Rabeau JR,Zvyagin AV,Deyev SM

更新日期：2013-05-01 00:00:00

abstract：:Carcinoma of the prostate (CaP) is the second leading cause of cancer-related mortality among American men. While high cure rates are associated with localized CaP, no cure exists for advanced recurrent disease. At present there are no known serologic biomarkers specific to this stage of the disease. Several groups ha...

journal_title：Proteomics

authors： Lam YW,Mobley JA,Evans JE,Carmody JF,Ho SM

更新日期：2005-07-01 00:00:00

abstract：:The International Protein Index (IPI) database has been one of the most widely used protein databases in MS proteomics approaches. Recently, the closure of IPI in September 2011 was announced. Its recommended replacement is the new UniProt Knowledgebase (UniProtKB) "complete proteome" sets, launched in May 2011. Here,...

journal_title：Proteomics

authors： Griss J,Martín M,O'Donovan C,Apweiler R,Hermjakob H,Vizcaíno JA

更新日期：2011-11-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a major complication of chronic viral hepatitis C. Therapy for HCC is still disappointing. It is thus of great importance to identify novel HCC markers for early detection of the disease, and tumor-specific proteins as potential therapeutic targets. We have used a proteomic approach t...

journal_title：Proteomics

authors： Blanc JF,Lalanne C,Plomion C,Schmitter JM,Bathany K,Gion JM,Bioulac-Sage P,Balabaud C,Bonneu M,Rosenbaum J

更新日期：2005-09-01 00:00:00

abstract：:New insights on cellular heterogeneity in the last decade provoke the development of a variety of single cell omics tools at a lightning pace. The resultant high-dimensional single cell data generated by these tools require new theoretical approaches and analytical algorithms for effective visualization and interpreta...

journal_title：Proteomics

authors： Su Y,Shi Q,Wei W

更新日期：2017-02-01 00:00:00

abstract：:Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood or bile contaminatio...

journal_title：Proteomics

authors： Zhou L,Lu Z,Yang A,Deng R,Mai C,Sang X,Faber KN,Lu X

更新日期：2007-04-01 00:00:00

abstract：:Despite affecting up to 70% of HIV-positive patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on...

journal_title：Proteomics

authors： Ganief T,Gqamana P,Garnett S,Hoare J,Stein DJ,Joska J,Soares N,Blackburn JM

更新日期：2017-03-01 00:00:00

abstract：:Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...

journal_title：Proteomics

authors： Josic D,Clifton JG

更新日期：2007-08-01 00:00:00

abstract：:To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that...

journal_title：Proteomics

authors： Osterman IA,Ustinov AV,Evdokimov DV,Korshun VA,Sergiev PV,Serebryakova MV,Demina IA,Galyamina MA,Govorun VM,Dontsova OA

更新日期：2013-01-01 00:00:00

abstract：:Protein interactions have been at the focus of computational biology in recent years. In particular, interest has come from two different communities--structural and systems biology. Here, we will discuss key systems and structural biology methods that have been used for analysis and prediction of protein-protein inte...

journal_title：Proteomics

authors： Hooda Y,Kim PM

更新日期：2012-05-01 00:00:00

abstract：:A characteristic of two-dimensional proteomics gels is a general bimodal distribution of isoelectric (pI) values. Discussion of this feature has focussed on the balance of acidic and basic ionisable residues, and potential relationships between pI distributions and organism classification or protein subcellular locati...

journal_title：Proteomics

authors： Chan P,Lovrić J,Warwicker J

更新日期：2006-06-01 00:00:00

abstract：:Unfortunately, conventional large-format polyacrylamide gels are mechanically fragile, often tearing during the subsequent manipulations required for visualization of the proteins. This problem is compounded when large-format two-dimensional gels are subjected to multiple staining procedures in order to detect differe...

journal_title：Proteomics

authors： Schulenberg B,Arnold B,Patton WF

更新日期：2003-07-01 00:00:00

abstract：:Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...

journal_title：Proteomics

authors： Finehout EJ,Cantor JR,Lee KH

更新日期：2005-06-01 00:00:00

abstract：:Integral membrane proteins (IMPs) perform crucial cellular functions and are the primary targets for most pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains and their intimate association with lipids make them difficult to handle. Numerous proteomic platforms that include LC sepa...

journal_title：Proteomics

authors： Blackler AR,Speers AE,Wu CC

更新日期：2008-10-01 00:00:00

abstract：:The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...

journal_title：Proteomics

authors： Bigeard J,Rayapuram N,Bonhomme L,Hirt H,Pflieger D

更新日期：2014-10-01 00:00:00