An improved mechanically durable electrophoresis gel matrix that is fully compatible with fluorescence-based protein detection technologies.

abstract：:Apoptosis contributes to cell death after cerebral ischaemia. A quantitative proteomics approach has been employed to define alterations in protein levels in apoptosis induced with staurosporine (STS). Human neuroblastoma derived SH-SY5Y cells were treated with STS (500 nM for 6 h) to induce apoptosis. Quantitative 2-...

journal_title：Proteomics

authors： Short DM,Heron ID,Birse-Archbold JL,Kerr LE,Sharkey J,McCulloch J

更新日期：2007-09-01 00:00:00

abstract：:Production of membrane proteins (MPs) is a challenging task as their hydrophobic nature and their specific requirements in cellular expression systems frequently prevent an efficient synthesis. Cell-free (CF) expression systems have been developed in recent times as promising tools by offering completely new approache...

journal_title：Proteomics

authors： Schwarz D,Dötsch V,Bernhard F

更新日期：2008-10-01 00:00:00

abstract：:MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...

journal_title：Proteomics

authors： Niggeweg R,Köcher T,Gentzel M,Buscaino A,Taipale M,Akhtar A,Wilm M

更新日期：2006-01-01 00:00:00

abstract：:With an emphasis on obtaining a multitude of high quality tandem mass spectrometry spectra for protein identification, instrumental parameters are described for the liquid chromatography-tandem mass spectrometry analysis of trypsin digested unfractionated urine using a hybrid quadrupole-time-of-flight (Q-TOF) mass spe...

journal_title：Proteomics

authors： Davis MT,Spahr CS,McGinley MD,Robinson JH,Bures EJ,Beierle J,Mort J,Yu W,Luethy R,Patterson SD

更新日期：2001-01-01 00:00:00

abstract：:Aluminum (Al) toxicity is a major constraint for plant root development and growth as well as crop yield in acidic soils, which constitute approximately 40% of the potentially arable lands worldwide. The mechanisms of Al tolerance in plants are not well understood. As a whole systems approach, proteomic techniques hav...

journal_title：Proteomics

authors： Zheng L,Lan P,Shen RF,Li WF

更新日期：2014-03-01 00:00:00

abstract：:Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, inclu...

journal_title：Proteomics

authors： Zhang J,Sui J,Ching CB,Chen WN

更新日期：2008-04-01 00:00:00

abstract：:Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells...

journal_title：Proteomics

authors： Khare T,Esteve-Núñez A,Nevin KP,Zhu W,Yates JR 3rd,Lovley D,Giometti CS

更新日期：2006-01-01 00:00:00

abstract：:Antibody microarrays have great potential for significant value in biological research. Cancer research in particular could benefit from the unique experimental capabilities of this technology. This article examines the current state of antibody microarray technological developments and assay formats, along with a rev...

journal_title：Proteomics

authors： Haab BB

更新日期：2003-11-01 00:00:00

abstract：:The Annual 2014 Spring Workshop of the Proteomics Standards Initiative (PSI) of the Human Proteome Organization (HUPO) was held this year jointly with the metabolomics COordination of Standards in MetabOlomicS (COSMOS) group. The range of existing MS standards (mzML, mzIdentML, mzQuantML, mzTab, TraML) was reviewed an...

journal_title：Proteomics

authors： Orchard S,Albar JP,Binz PA,Kettner C,Jones AR,Salek RM,Vizcaino JA,Deutsch EW,Hermjakob H

更新日期：2014-11-01 00:00:00

abstract：:Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...

journal_title：Proteomics

authors： Kim SY,Joglekar MV,Hardikar AA,Phan TH,Khanal D,Tharkar P,Limantoro C,Johnson J,Kalionis B,Chrzanowski W

更新日期：2019-09-01 00:00:00

abstract：:Ovarian cancer is one of the leading causes of mortality by gynecological cancer. Despite good response to surgery and initial chemotherapy, essentially based on cisplatin (cis-diamino-dichloro-platinum(II) (CDDP)) compounds, frequent recurrences with chemoresistance acquisition are responsible for poor prognosis. Sev...

journal_title：Proteomics

authors： Le Moguen K,Lincet H,Marcelo P,Lemoisson E,Heutte N,Duval M,Poulain L,Vinh J,Gauduchon P,Baudin B

更新日期：2007-11-01 00:00:00

abstract：:This paper proposes a novel profiling method for SELDI-TOF and MALDI-TOF MS data that integrates a novel peak detection method based on modified smoothed non-linear energy operator, correlation-based peak selection and Bayesian additive regression trees. The peak detection and classification performance of the propose...

journal_title：Proteomics

authors： He S,Li X,Viant MR,Yao X

更新日期：2009-09-01 00:00:00

abstract：:Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...

journal_title：Proteomics

authors： Nguyen-Kim H,San Clemente H,Balliau T,Zivy M,Dunand C,Albenne C,Jamet E

更新日期：2016-02-01 00:00:00

abstract：:Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. I...

journal_title：Proteomics

authors： Gilbert I,Schiffmann S,Rubenwolf S,Jensen K,Mai T,Albrecht C,Lankenau A,Beste G,Blank K,Gaub HE,Clausen-Schaumann H

更新日期：2004-05-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are implicated in a range of human diseases, some of which are associated with the ability to bind to lipids. Although the presence of solvent-exposed hydrophobic regions in IDPs should favor their interactions with low-molecular-weight hydrophobic/amphiphilic compounds, this h...

journal_title：Proteomics

authors： Deryusheva E,Nemashkalova E,Galloux M,Richard CA,Eléouët JF,Kovacs D,Van Belle K,Tompa P,Uversky V,Permyakov S

更新日期：2019-03-01 00:00:00

abstract：:Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...

journal_title：Proteomics

authors： Aziz MM,Maleki M,Rueda L,Raza M,Banerjee S

更新日期：2011-10-01 00:00:00

abstract：:Current treatments for advanced prostate cancer focus on inhibition of the androgen receptor (AR) by androgen deprivation therapy (ADT). However, complex interactions mediated by tumor suppressors, oncogenes, aberrations of AR expression, or de novo androgen production have been shown to induce the adaptive response o...

journal_title：Proteomics

authors： Soekmadji C,Rockstroh A,Ramm GA,Nelson CC,Russell PJ

更新日期：2017-12-01 00:00:00

abstract：:The monitoring of gene expression via the technologies encompassed under the term 'proteomics' allows proteins of significance to be related to phenotypes associated with strain variability, environmental influences and the effects of genetic manipulation. The characterizations of these molecules are routinely perform...

journal_title：Proteomics

authors： Cordwell SJ,Nouwens AS,Walsh BJ

更新日期：2001-04-01 00:00:00

abstract：:Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is still limited. This is because we have so far mostly relied on g...

journal_title：Proteomics

authors： Simicevic J,Deplancke B

更新日期：2017-02-01 00:00:00

abstract：:Adiponectin is a fat cell-secreted hormone with antidiabetic and anti-inflammatory activities. The reduced adiponectin levels are associated with obesity-related metabolic syndrome. Replenishment of this hormone into animal models can improve insulin sensitivity, decrease blood glucose and lipid levels, and prevent th...

journal_title：Proteomics

authors： Wang Y,Xu LY,Lam KS,Lu G,Cooper GJ,Xu A

更新日期：2006-07-01 00:00:00

abstract：:Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...

journal_title：Proteomics

authors： Ahmed N,Barker G,Oliva K,Garfin D,Talmadge K,Georgiou H,Quinn M,Rice G

更新日期：2003-10-01 00:00:00

abstract：:Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...

journal_title：Proteomics

authors： Josic D,Clifton JG

更新日期：2007-08-01 00:00:00

abstract：:During early stages of embryo development, the brain cavity is filled with embryonic cerebrospinal fluid (E-CSF), a complex fluid containing different protein fractions that contributes to the regulation of the survival, proliferation and neurogenesis of the neuroectodermal stem cells. Using 2-DE, protein sequencing a...

journal_title：Proteomics

authors： Parada C,Gato A,Aparicio M,Bueno D

更新日期：2006-01-01 00:00:00

abstract：:FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...

journal_title：Proteomics

authors： Gattiker A,Bienvenut WV,Bairoch A,Gasteiger E

更新日期：2002-10-01 00:00:00

abstract：:Secreted proteins play a pivotal role in cellular functions. To better understand malignant behavior, we adapted stable isotopic labeling with amino acids in cell culture technology to identify and quantify proteins differentially released into the extracellular media by a pair of normal and malignant breast-cancer ce...

journal_title：Proteomics

authors： Liang X,Huuskonen J,Hajivandi M,Manzanedo R,Predki P,Amshey JR,Pope RM

更新日期：2009-01-01 00:00:00

abstract：:Calcineurin is a heterodimeric serine/threonine protein phosphatase, important for many cellular processes such as T-cell regulation, cardiac hypertrophy and kidney development. We previously reported the characterization of Caenorhabditis elegans calcineurin mutants as providing a simple but excellent genetic model s...

journal_title：Proteomics

authors： Ahn DH,Singaravelu G,Lee S,Ahnn J,Shim YH

更新日期：2006-02-01 00:00:00

abstract：:In the present study, changes in protein synthesis patterns after salt shock visualized by 35S-methionine labeling and the changed protein composition in salt-acclimated cells of the cyanobacterium Synechocystis sp. strain PCC 6803 were analyzed by a combination of 2-DE for protein separation and PMF for protein ident...

journal_title：Proteomics

authors： Fulda S,Mikkat S,Huang F,Huckauf J,Marin K,Norling B,Hagemann M

更新日期：2006-05-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...

journal_title：Proteomics

authors： Sidahmed-Adrar N,Ottavi JF,Benzoubir N,Ait Saadi T,Bou Saleh M,Mauduit P,Guettier C,Desterke C,Le Naour F

更新日期：2019-11-01 00:00:00

abstract：:Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...

journal_title：Proteomics

authors： Riwaldt S,Pietsch J,Sickmann A,Bauer J,Braun M,Segerer J,Schwarzwälder A,Aleshcheva G,Corydon TJ,Infanger M,Grimm D

更新日期：2015-09-01 00:00:00

abstract：:Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...

journal_title：Proteomics

authors： Erce MA,Pang CN,Hart-Smith G,Wilkins MR

更新日期：2012-02-01 00:00:00