The making of an embryo in a basal metazoan: Proteomic analysis in the sea anemone Nematostella vectensis.

abstract：:Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

更新日期：2009-07-01 00:00:00

abstract：:Mitochondria were isolated from whole hearts of Dahl salt sensitive (SS) and chromosome 13 consomic control (SS.13BN/Mcwi) rats using a mechanical homogenization process followed by density centrifugation. The proteins present in the two mitochondria preparations were quantified; equal amounts of protein from each sam...

journal_title：Proteomics

authors： Smith JR,Matus IR,Beard DA,Greene AS

更新日期：2008-02-01 00:00:00

abstract：:Four rhoptry proteins (ROP) of Toxoplasma gondii previously identified with mAb have been affinity purified and analyzed by MS; the data obtained allowed the genomic sequences to be assigned to these proteins. As previously suggested for some of them by antibody crossreactivity, these proteins were shown to belong to ...

journal_title：Proteomics

authors： El Hajj H,Demey E,Poncet J,Lebrun M,Wu B,Galéotti N,Fourmaux MN,Mercereau-Puijalon O,Vial H,Labesse G,Dubremetz JF

更新日期：2006-11-01 00:00:00

abstract：:Large-scale interaction studies contribute the largest fraction of protein interactions information in databases. However, co-purification of non-specific or indirect ligands, often results in data sets that are affected by a considerable number of false positives. For the fraction of interactions mediated by short li...

journal_title：Proteomics

authors： Panni S,Montecchi-Palazzi L,Kiemer L,Cabibbo A,Paoluzi S,Santonico E,Landgraf C,Volkmer-Engert R,Bachi A,Castagnoli L,Cesareni G

更新日期：2011-01-01 00:00:00

abstract：:Cells are a fundamental unit of life, and the ability to study the phenotypes and behaviors of individual cells is crucial to understanding the workings of complex biological systems. Cell phenotypes (epigenomic, transcriptomic, proteomic, and metabolomic) exhibit dramatic heterogeneity between and within the differen...

journal_title：Proteomics

authors： Mincarelli L,Lister A,Lipscombe J,Macaulay IC

更新日期：2018-09-01 00:00:00

abstract：:Prostate cancer is one of the leading cancers in men. Taking dietary supplements, such as fish oil (FO), which is rich in n-3 polyunsaturated fatty acids (PUFAs), has been employed as a strategy to lower prostate cancer risk and control disease progression. In this study, we investigated the global phosphoproteomic ch...

journal_title：Proteomics

authors： Zhao H,Pflug BR,Lai X,Wang M

更新日期：2016-09-01 00:00:00

abstract：:A proteome wide analysis was performed in Escherichia coli to identify the impact on protein N-termini of actinonin, an antibiotic specifically inhibiting peptide deformylase (PDF). A strategy and tool suite (SILProNaQ) was employed to provide large-scale quantitation of N-terminal modifications. In control conditions...

journal_title：Proteomics

authors： Bienvenut WV,Giglione C,Meinnel T

更新日期：2015-07-01 00:00:00

abstract：:Sandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust-affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single-chain antibodies (scFv) are affinity reagents that have greater potential for high-throughput p...

journal_title：Proteomics

authors： Seurynck-Servoss SL,Baird CL,Miller KD,Pefaur NB,Gonzalez RM,Apiyo DO,Engelmann HE,Srivastava S,Kagan J,Rodland KD,Zangar RC

更新日期：2008-06-01 00:00:00

abstract：:The flavonoid flavone contained in a variety of fruits and vegetables was identified as a very potent apoptosis inducer in human colonic cancer cells. In search of the molecular targets of flavone action in HT-29 cells we analyzed changes in mRNA and protein expression levels by proteomics and oligonucleotide array te...

journal_title：Proteomics

authors： Herzog A,Kindermann B,Döring F,Daniel H,Wenzel U

更新日期：2004-08-01 00:00:00

abstract：:Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans. In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to char...

journal_title：Proteomics

authors： You X,Qin H,Mao J,Tian Y,Dong M,Guo Z,Liang X,Wang L,Jin Y,Ye M

更新日期：2018-09-01 00:00:00

abstract：:Proteins determine the quality of barley in malting and brewing end-uses. In this regard, water-soluble barley proteins play a major role in the formation, stability, and texture of head foams. Our objective was to survey the barley seed proteins that could be involved in the foaming properties of beer. Therefore, two...

journal_title：Proteomics

authors： Perrocheau L,Rogniaux H,Boivin P,Marion D

更新日期：2005-07-01 00:00:00

abstract：:Antibody-based proteomics applied to tissue microarray (TMA) technology provides a very efficient means of visualizing and locating antigen expression in large collections of normal and pathological tissue samples. To characterize antigen expression on TMAs, the use of image analysis methods avoids the effects of huma...

journal_title：Proteomics

authors： Decaestecker C,Lopez XM,D'Haene N,Roland I,Guendouz S,Duponchelle C,Berton A,Debeir O,Salmon I

更新日期：2009-10-01 00:00:00

abstract：:Protein quantification at proteome-wide scale is an important aim, enabling insights into fundamental cellular biology and serving to constrain experiments and theoretical models. While proteome-wide quantification is not yet fully routine, many datasets approaching proteome-wide coverage are becoming available throug...

journal_title：Proteomics

authors： Wang M,Herrmann CJ,Simonovic M,Szklarczyk D,von Mering C

更新日期：2015-09-01 00:00:00

abstract：:This study aimed at establishing a sensitive multiple reaction monitoring-mass spectrometry (MRM-MS) method for the quantification of the drug metabolizing cytochrome P450 (CYP)3A4 enzyme in human liver homogenates. Liver samples were subjected to trypsin digestion. MRM-MS analyses were performed using three transitio...

journal_title：Proteomics

authors： Cieślak A,Kelly I,Trottier J,Verreault M,Wunsch E,Milkiewicz P,Poirier G,Droit A,Barbier O

更新日期：2016-11-01 00:00:00

abstract：:Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...

journal_title：Proteomics

authors： Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RD

更新日期：2012-04-01 00:00:00

abstract：:Most MS-based glycomic and glycoproteomic analyses focus on identifying changes in terminal glyco-epitopes represented by sialylation and fucosylation at specific positions of the terminal N-acetyllactosamine units. Much less attention was accorded to the underlying linear or branched poly-N-acetyllactosamine extensio...

journal_title：Proteomics

authors： Wang SH,Wu SW,Khoo KH

更新日期：2011-07-01 00:00:00

abstract：:Unconjugated bilirubin (UCB) is a powerful antioxidant and a modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop a physiological jaundice, in case of severe hyperbilirubinemia UCB may become neurotoxic causing severe long-term neuronal damages, also kno...

journal_title：Proteomics

authors： Deganuto M,Cesaratto L,Bellarosa C,Calligaris R,Vilotti S,Renzone G,Foti R,Scaloni A,Gustincich S,Quadrifoglio F,Tiribelli C,Tell G

更新日期：2010-04-01 00:00:00

abstract：:Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due ...

journal_title：Proteomics

authors： Yuan C,Jiao L,Yang L,Ying W,Hu Z,Liu J,Cui F,Li L,Qian L,Teng Y,Hang H,Qian X,Yang X

更新日期：2008-06-01 00:00:00

abstract：:The function of a protein is modulated by its abundance and its degree of specific post-translational modifications such as phosphorylation, glycosylation or truncation. Consequently, changes of protein concentration and the extent of their post-translational modifications has a great influence on the activity of intr...

journal_title：Proteomics

authors： Moritz B,Meyer HE

更新日期：2003-11-01 00:00:00

abstract：:Anaeromyxobacter dehalogenans is a microaerophilic member of the delta-proteobacteria which is able to utilize a wide range of electron acceptors, including halogenated phenols, U(VI), Fe(III), nitrate, nitrite, oxygen and fumarate. To date, the knowledge regarding general metabolic activities of this ecologically rel...

journal_title：Proteomics

authors： Chao TC,Kalinowski J,Nyalwidhe J,Hansmeier N

更新日期：2010-04-01 00:00:00

abstract：:Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...

journal_title：Proteomics

authors： Jackson CA,Yadav N,Min S,Li J,Milliman EJ,Qu J,Chen YC,Yu MC

更新日期：2012-11-01 00:00:00

abstract：:Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (...

journal_title：Proteomics

authors： Wang MC,Peng ZY,Li CL,Li F,Liu C,Xia GM

更新日期：2008-04-01 00:00:00

abstract：:Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...

journal_title：Proteomics

authors： Ofir K,Berdichevsky Y,Benhar I,Azriel-Rosenfeld R,Lamed R,Barak Y,Bayer EA,Morag E

更新日期：2005-05-01 00:00:00

abstract：:The identification of specific protein markers for colorectal cancer would provide the basis for early diagnosis and detection, as well as clues for understanding the molecular mechanisms governing cancer progression. In this report, we describe the proteomic analysis of the samples of colorectal cancer corresponding ...

journal_title：Proteomics

authors： Alfonso P,Núñez A,Madoz-Gurpide J,Lombardia L,Sánchez L,Casal JI

更新日期：2005-07-01 00:00:00

abstract：:RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock-down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interferenc...

journal_title：Proteomics

authors： Özbalci C,Storck EM,Eggert US

更新日期：2019-07-01 00:00:00

abstract：:Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...

journal_title：Proteomics

authors： Liu K,Zhang J,Fu B,Xie H,Wang Y,Qian X

更新日期：2014-07-01 00:00:00

abstract：:Biological membranes are compartmentalized into microdomains that exhibit particular lipid and protein compositions. Membrane microdomains, such as tetraspanin-enriched microdomains and lipid rafts, have been suggested to play a role in a variety of physiological and pathological processes. Therefore, the characteriza...

journal_title：Proteomics

authors： Le Naour F,André M,Boucheix C,Rubinstein E

更新日期：2006-12-01 00:00:00

abstract：:MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...

journal_title：Proteomics

authors： Helsens K,Martens L,Vandekerckhove J,Gevaert K

更新日期：2007-02-01 00:00:00

abstract：:Proteolytic modification of components of the extracellular milieu by metalloproteinases plays important roles in the regulation of multiple cellular and physiological processes and pathological conditions. ADAMTS1 is a secreted enzyme of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) fami...

journal_title：Proteomics

authors： Canals F,Colomé N,Ferrer C,Plaza-Calonge Mdel C,Rodríguez-Manzaneque JC

更新日期：2006-04-01 00:00:00

abstract：:Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...

journal_title：Proteomics

authors： Westers L,Westers H,Zanen G,Antelmann H,Hecker M,Noone D,Devine KM,van Dijl JM,Quax WJ

更新日期：2008-07-01 00:00:00