Consequences of the discontinuation of the International Protein Index (IPI) database and its substitution by the UniProtKB "complete proteome" sets.

abstract：:Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...

journal_title：Proteomics

authors： Gao Y,Lee H,Kwon OK,Cheng Z,Tan M,Kim KT,Lee S

更新日期：2019-05-01 00:00:00

abstract：:Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-bas...

journal_title：Proteomics

authors： Khatabi B,Gharechahi J,Ghaffari MR,Liu D,Haynes PA,McKay MJ,Mirzaei M,Salekdeh GH

更新日期：2019-08-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a major complication of chronic viral hepatitis C. Therapy for HCC is still disappointing. It is thus of great importance to identify novel HCC markers for early detection of the disease, and tumor-specific proteins as potential therapeutic targets. We have used a proteomic approach t...

journal_title：Proteomics

authors： Blanc JF,Lalanne C,Plomion C,Schmitter JM,Bathany K,Gion JM,Bioulac-Sage P,Balabaud C,Bonneu M,Rosenbaum J

更新日期：2005-09-01 00:00:00

abstract：:FtsH is the only membrane-bound and essential protease in Escherichia coli. It is responsible for the degradation of regulatory proteins and enzymes such as the heat-shock sigma factor RpoH or LpxC, the key enzyme of lipopolysaccharide biosynthesis. To find new FtsH targets, we trapped substrates in E. coli cells from...

journal_title：Proteomics

authors： Arends J,Thomanek N,Kuhlmann K,Marcus K,Narberhaus F

更新日期：2016-12-01 00:00:00

abstract：:Mulberry dwarf (MD) is a serious infectious disease of mulberry caused by phytoplasma. Infection with MD phytoplasma results in stress phenotypes of yellowing, phyllody, stunting, proliferation, and witches' broom. Physiological and biochemical analysis has shown that infection with MD phytoplasma causes an increase i...

journal_title：Proteomics

authors： Ji X,Gai Y,Zheng C,Mu Z

更新日期：2009-12-01 00:00:00

abstract：:Oligogalacturonides (OGs) are elicitors of plant defence responses released from the homogalacturonan of the plant cell wall during the attack by pathogenic micro-organisms. The signalling pathway mediated by OGs remains poorly understood, and no proteins involved in their signal perception and transduction have yet b...

journal_title：Proteomics

authors： Casasoli M,Spadoni S,Lilley KS,Cervone F,De Lorenzo G,Mattei B

更新日期：2008-03-01 00:00:00

abstract：:Depletion of abundant proteins is one of the effective ways to improve detection and identification of low-abundance proteins. Our previous study showed that protamine sulfate precipitation (PSP) method can deplete abundant ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from leaf proteins and is suitable fo...

journal_title：Proteomics

authors： Kim YJ,Wang Y,Gupta R,Kim SW,Min CW,Kim YC,Park KH,Agrawal GK,Rakwal R,Choung MG,Kang KY,Kim ST

更新日期：2015-05-01 00:00:00

abstract：:Roots, leaves, and intermediate sections between roots and leaves (ISRL) of wheat seedlings show different physiological functions at the protein level. We performed the first integrative proteomic analysis of different tissues of the drought-tolerant wheat cultivar Hanxuan 10 (HX-10) and drought-sensitive cultivar Ch...

journal_title：Proteomics

authors： Hao P,Zhu J,Gu A,Lv D,Ge P,Chen G,Li X,Yan Y

更新日期：2015-05-01 00:00:00

abstract：:In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons: Low price, Visibl...

journal_title：Proteomics

authors： Westermeier R

更新日期：2006-09-01 00:00:00

abstract：:Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...

journal_title：Proteomics

authors： Holzlechner M,Reitschmidt S,Gruber S,Zeilinger S,Marchetti-Deschmann M

更新日期：2016-06-01 00:00:00

abstract：:In the post-genomic era, mass spectrometry is destined to fulfil a central role in biomedical research, and it is in the area of protein identification that mass spectrometry is now most rapidly expanding. An important identification method is to subject a protein to proteolysis and determine the resulting peptide mas...

journal_title：Proteomics

authors： Griffiths WJ,Jonsson AP

更新日期：2001-08-01 00:00:00

abstract：:This review is a meta-analysis of data describing proteins regulated by morphine influence studied worldwide across last years administration. Up to date (July 2010), 15 studies concerning this subject have been published. Animal models, examined brain structures, the route of morphine administration and proteomic pla...

journal_title：Proteomics

authors： Bodzon-Kułakowska A,Kułakowski K,Drabik A,Moszczynski A,Silberring J,Suder P

更新日期：2011-01-01 00:00:00

abstract：:Brain development and aging is a complex process involving proliferation, differentiation and apoptosis. Elucidating proteome changes in these processes can help to understand the mechanisms of brain development and maintenance as well as neurodegenerative diseases. The research reported here is a contribution to the ...

journal_title：Proteomics

authors： Föcking M,Boersema PJ,O'Donoghue N,Lubec G,Pennington SR,Cotter DR,Dunn MJ

更新日期：2006-09-01 00:00:00

abstract：:Toxic oil syndrome (TOS) is a disease that appeared in Spain in 1981. It affected more than 20 000 people and produced over 300 deaths in the first 2 years. In this paper, a prospective study on the differences in gene expression in sera between a control versus a TOS-affected population, both originally exposed to th...

journal_title：Proteomics

authors： Quero C,Colomé N,Prieto MR,Carrascal M,Posada M,Gelpí E,Abian J

更新日期：2004-02-01 00:00:00

abstract：:Tyrosol (TYR, 2-(4-hydroxyphenyl)ethanol), one of the main phenols in olive oil and olive fruit, significantly strengthens resistance to thermal and oxidative stress in the nematode Caenorhabditis elegans and extends its lifespan. To elucidate the cellular functions regulated by TYR, we have used a proteomic procedure...

journal_title：Proteomics

authors： Cañuelo A,Peragón J

更新日期：2013-10-01 00:00:00

abstract：:In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...

journal_title：Proteomics

authors： Grimplet J,Wheatley MD,Jouira HB,Deluc LG,Cramer GR,Cushman JC

更新日期：2009-05-01 00:00:00

abstract：:Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs)...

journal_title：Proteomics

authors： Evans J,Rai A,Nguyen HPT,Poh QH,Elglass K,Simpson RJ,Salamonsen LA,Greening DW

更新日期：2019-12-01 00:00:00

abstract：:In a comparative study, we investigated the influence of nine sample preparation workflows and seven different lysis buffers for qualitative and quantitative analysis of the human adipose tissue proteome. Adipose tissue is not just a fat depot but also an endocrine organ, which cross-talks with other tissue types and ...

journal_title：Proteomics

authors： Pasing Y,Colnoe S,Hansen T

更新日期：2017-03-01 00:00:00

abstract：:The last years have seen the emergence of many large-scale proteomics initiatives that have identified thousands of new protein interactions and macromolecular assemblies. However, unfortunately, only a few among the discovered complexes meet the high-quality standards required to be promptly used in structural studie...

journal_title：Proteomics

authors： Pache RA,Aloy P

更新日期：2008-05-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry (MS/MS) were used to determine the multiple acetylation sites in the histone acetyltransferase (HAT): p300-HAT. Partial cleavage of the peptides containing acetylated lysine residues by trypsin provided a set of nested sequences...

journal_title：Proteomics

authors： Wang D,Thompson P,Cole PA,Cotter RJ

更新日期：2005-06-01 00:00:00

abstract：:Proteases are critical in many physiological processes and the human genome encodes for 566 predicted proteolytic enzymes. Therefore, there is great interest in identifying and characterizing physiologic protease-substrate relationships. The coagulation cascade is a well-described network of serine proteases. However,...

journal_title：Proteomics

authors： Niessen S,Hoover H,Gale AJ

更新日期：2011-06-01 00:00:00

abstract：:A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...

journal_title：Proteomics

authors： Huelseweh B,Ehricht R,Marschall HJ

更新日期：2006-05-01 00:00:00

abstract：:Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...

journal_title：Proteomics

authors： Agrawal GK,Rakwal R,Yonekura M,Kubo A,Saji H

更新日期：2002-08-01 00:00:00

abstract：:Scab, caused by Fusarium graminearum, is a serious spike disease in wheat. To identify proteins in resistant wheat cultivar Wangshuibai induced by F. graminearum infection, proteins extracted from spikes 6, 12 and 24 h after inoculation were separated by 2-DE. Thirty protein spots showing 3-fold change in abundance wh...

journal_title：Proteomics

authors： Wang Y,Yang L,Xu H,Li Q,Ma Z,Chu C

更新日期：2005-11-01 00:00:00

abstract：:Chinese tarantula, Chilobrachys jingzhao is one of the most venomous spiders in southern China and its venom is a mixture of various compounds with diversified biological activities. The proteome of C. jingzhao venom was analyzed by proteomic techniques. Proteins with molecular weight of over 10 kDa, indicated by gel-...

journal_title：Proteomics

authors： Liao Z,Cao J,Li S,Yan X,Hu W,He Q,Chen J,Tang J,Xie J,Liang S

更新日期：2007-06-01 00:00:00

abstract：:Recently, we introduced a novel proteomics method employing a metalloendopeptidase with Lys-N specificity to produce proteolytic peptides. Fragmentation spectra generated by electron transfer dissociation, for a large proportion of the Lys-N proteolytic peptides, were found to be dominated by extensive series of c-typ...

journal_title：Proteomics

authors： van Breukelen B,Georgiou A,Drugan MM,Taouatas N,Mohammed S,Heck AJ

更新日期：2010-03-01 00:00:00

abstract：:Previously, we reported that nucleophosmin (NPM) was increased in glioblastoma multiforme (GBM). NPM is a phosphoprotein related to apoptosis, ribosome biogenesis, mitosis, and DNA repair, but details about its function remain unclear. We treated U87MG and A172 cells with small interference RNA (siRNA) and obtained a ...

journal_title：Proteomics

authors： Gimenez M,Marie SK,Oba-Shinjo SM,Uno M,da Silva R,Laure HJ,Izumi C,Otake A,Chammas R,Rosa JC

更新日期：2012-08-01 00:00:00

abstract：:One of the major challenges facing protein analysis is the dynamic range of protein expression within massively complex samples (Corthals, G. L. et al.., Electrophoresis 2000, 21, 1104-1115). In plasma this difference is as great as ten orders of magnitude, and this is currently beyond the range of detection achievabl...

journal_title：Proteomics

authors： Wasinger VC,Locke VL,Raftery MJ,Larance M,Rothemund D,Liew A,Bate I,Guilhaus M

更新日期：2005-08-01 00:00:00

abstract：:Currently, there are few predictive biomarkers in key biomonitoring species, such as oysters, that can detect heavy metal pollution in coastal waterways. Several attributes make oysters superior to other organisms for positive biomonitoring of heavy metal pollution. In particular, they are filter feeders with a high c...

journal_title：Proteomics

authors： Muralidharan S,Thompson E,Raftos D,Birch G,Haynes PA

更新日期：2012-03-01 00:00:00

abstract：:DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...

journal_title：Proteomics

authors： Timms JF,Cramer R

更新日期：2008-12-01 00:00:00