Morphinome--a meta-analysis applied to proteomics studies in morphine dependence.

abstract：:Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of these channels is tightly controlled by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signaling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP2 ), Ca2+ /calmodulin, and pho...

journal_title：Proteomics

authors： Erdem FA,Salzer I,Heo S,Chen WQ,Jung G,Lubec G,Boehm S,Yang JW

更新日期：2017-10-01 00:00:00

abstract：:Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...

journal_title：Proteomics

authors： Holzlechner M,Reitschmidt S,Gruber S,Zeilinger S,Marchetti-Deschmann M

更新日期：2016-06-01 00:00:00

abstract：:With the human Plasma Proteome Project (PPP) pilot phase completed, the largest and most ambitious proteomics experiment to date has reached its first milestone. The correspondingly impressive amount of data that came from this pilot project emphasized the need for a centralized dissemination mechanism and led to the ...

journal_title：Proteomics

authors： Martens L,Nesvizhskii AI,Hermjakob H,Adamski M,Omenn GS,Vandekerckhove J,Gevaert K

更新日期：2005-08-01 00:00:00

abstract：:Biotherapeutics, molecules produced from biological systems, require rigorous purification steps to remove impurities including host cell proteins (HCPs). Regulatory guidelines require manufacturers to monitor process-related impurities along the purification workflow. Mass spectrometry (MS) has recently been consider...

journal_title：Proteomics

authors： Trauchessec M,Hesse AM,Kraut A,Berard Y,Herment L,Fortin T,Bruley C,Ferro M,Manin C

更新日期：2021-01-18 00:00:00

abstract：:Malt derived from barley malting is an essential raw material for beer brewing. In this study, we performed the first dynamic proteome survey during barley malting using a gel-free proteomics approach. This entailed in-solution tryptic digestion of precipitated proteins and analysis of peptides by nanoliquid chromatog...

journal_title：Proteomics

authors： Mahalingam R

更新日期：2018-08-01 00:00:00

abstract：:The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...

journal_title：Proteomics

authors： Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh J

更新日期：2006-11-01 00:00:00

abstract：:Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...

journal_title：Proteomics

authors： Ofir K,Berdichevsky Y,Benhar I,Azriel-Rosenfeld R,Lamed R,Barak Y,Bayer EA,Morag E

更新日期：2005-05-01 00:00:00

abstract：:The hepatitis B virus X-protein (HBx), a multifunctional viral regulator, participates in the viral life cycle and in the development of hepatocellular carcinoma (HCC). We previously reported a high incidence of HCC in transgenic mice expressing HBx. In this study, proteomic analysis was performed to identify proteins...

journal_title：Proteomics

authors： Kim SY,Lee PY,Shin HJ,Kim DH,Kang S,Moon HB,Kang SW,Kim JM,Park SG,Park BC,Yu DY,Bae KH,Lee SC

更新日期：2009-11-01 00:00:00

abstract：:Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. Ho...

journal_title：Proteomics

authors： Zavan L,Bitto NJ,Johnston EL,Greening DW,Kaparakis-Liaskos M

更新日期：2019-01-01 00:00:00

abstract：:Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...

journal_title：Proteomics

authors： Davoodi J,Kelly J,Gendron NH,MacKenzie AE

更新日期：2007-06-01 00:00:00

abstract：:Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...

journal_title：Proteomics

authors： Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

更新日期：2005-04-01 00:00:00

abstract：:As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restr...

journal_title：Proteomics

authors： Ge P,Hao P,Cao M,Guo G,Lv D,Subburaj S,Li X,Yan X,Xiao J,Ma W,Yan Y

更新日期：2013-10-01 00:00:00

abstract：:Pathogenic bacteria excrete a variety of virulence factors into extracellular medium and to the cell surface which have essential roles in the colonization and insurrection of the host cells, and thus reflect the degree of bacterial pathogenicity. For the exploration of virulence factors expressed in the secreted prot...

journal_title：Proteomics

authors： Pocsfalvi G,Cacace G,Cuccurullo M,Serluca G,Sorrentino A,Schlosser G,Blaiotta G,Malorni A

更新日期：2008-06-01 00:00:00

abstract：:In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...

journal_title：Proteomics

authors： Grimplet J,Wheatley MD,Jouira HB,Deluc LG,Cramer GR,Cushman JC

更新日期：2009-05-01 00:00:00

abstract：:Heat shock proteins (HSP) are families of highly conserved proteins which are induced in cells and tissues upon exposure to extreme conditions causing acute or chronic stress. They exhibit distinct functions and have been implicated in the pathogenesis of a number of diseases, including cancer. A causal relationship b...

journal_title：Proteomics

authors： Lichtenfels R,Kellner R,Bukur J,Beck J,Brenner W,Ackermann A,Seliger B

更新日期：2002-05-01 00:00:00

abstract：:The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established t...

journal_title：Proteomics

authors： Fang JY,Wang PW,Huang CH,Chen MH,Wu YR,Pan TL

更新日期：2016-10-01 00:00:00

abstract：:A method for quantitative proteomic analysis based on the selective isolation of multiply charged peptides (RH peptides) containing arginine and histidine residues is described. Two pools of proteins are digested in tandem with lysyl-endopeptidase and trypsin and the primary amino groups of proteolytic peptides are se...

journal_title：Proteomics

authors： Sánchez A,González LJ,Betancourt L,Gil J,Besada V,Fernández-de-Cossío J,Rodríguez-Ulloa A,Marrero K,Alvarez F,Fando R,Padrón G

更新日期：2006-08-01 00:00:00

abstract：:Leishmaniasis is a zoonotic disease caused by the species of the genus Leishmania, flagellated protozoa that multiply inside mammalian macrophages and are transmitted by the bite of the sandfly. The disease is widespread and due to the lack of fully effective treatment and vaccination the search for new drugs and immu...

journal_title：Proteomics

authors： Dea-Ayuela MA,Rama-Iñiguez S,Bolás-Fernández F

更新日期：2006-07-01 00:00:00

abstract：:Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of im...

journal_title：Proteomics

authors： Luk VN,Fiddes LK,Luk VM,Kumacheva E,Wheeler AR

更新日期：2012-05-01 00:00:00

abstract：:The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...

journal_title：Proteomics

authors： Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

更新日期：2012-06-01 00:00:00

abstract：:Entamoeba histolytica is known for its extraordinary capacity to destroy human tissues, leading to invasive diseases such as ulcerative colitis or extra-intestinal abscesses. In order to identify the virulence factors of this parasite phenotypes and proteomes of two recently identified genetically related cell lines (...

journal_title：Proteomics

authors： Biller L,Schmidt H,Krause E,Gelhaus C,Matthiesen J,Handal G,Lotter H,Janssen O,Tannich E,Bruchhaus I

更新日期：2009-09-01 00:00:00

abstract：:Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on complex and time-consuming protein purification and reduction re...

journal_title：Proteomics

authors： Khairallah RJ,Sadayappan S

更新日期：2013-11-01 00:00:00

abstract：:The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...

journal_title：Proteomics

authors： Lubelwana Hafver T,Wanichawan P,Manfra O,de Souza GA,Lunde M,Martinsen M,Louch WE,Sejersted OM,Carlson CR

更新日期：2017-09-01 00:00:00

abstract：:Two-dimensional difference gel electrophoresis (2-D DIGE) allows for reliable quantification of global protein abundance changes. The threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). This study estimates biological, technical and total variation ...

journal_title：Proteomics

authors： Zech H,Echtermeyer C,Wöhlbrand L,Blasius B,Rabus R

更新日期：2011-08-01 00:00:00

abstract：:Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a 2-DE-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsa...

journal_title：Proteomics

authors： Singh OV,Yaster M,Xu JT,Guan Y,Guan X,Dharmarajan AM,Raja SN,Zeitlin PL,Tao YX

更新日期：2009-03-01 00:00:00

abstract：:Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an ...

journal_title：Proteomics

authors： Schueder F,Unterauer EM,Ganji M,Jungmann R

更新日期：2020-10-08 00:00:00

abstract：:We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...

journal_title：Proteomics

authors： Goodwin RJ,Dungworth JC,Cobb SR,Pitt AR

更新日期：2008-09-01 00:00:00

abstract：:Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrom...

journal_title：Proteomics

authors： Nissum M,Kuhfuss S,Hauptmann M,Obermaier C,Sukop U,Wildgruber R,Weber G,Eckerskorn C,Malmström J

更新日期：2007-12-01 00:00:00

abstract：:Selective activation of T helper subsets 1 (Th1) and 2 (Th2) plays a crucial role in different pathological conditions. Th1 cell response is involved in pathogenesis of autoimmune diseases, such as type II diabetes and multiple sclerosis, and Th2 cell response in pathogenesis of allergy and asthma. Cytokine interleuki...

journal_title：Proteomics

authors： Rosengren AT,Nyman TA,Lahesmaa R

更新日期：2005-08-01 00:00:00

abstract：:The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic dig...

journal_title：Proteomics

authors： Gundry RL,Tchernyshyov I,Sheng S,Tarasova Y,Raginski K,Boheler KR,Van Eyk JE

更新日期：2010-07-01 00:00:00