Abstract:
:Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on complex and time-consuming protein purification and reduction reactions to yield useful information. In this issue of Proteomics, Zhao et al. (high-throughput screening of disulfide-containing proteins in a complex mixture, Proteomics 2013, 13, 3256-3260) devise a clever and rapid method for high-throughput determination of disulfides in proteins via reduction by tris(2-carboxyethyl)phosphine. Their work provides the foundation necessary to undertake more complex experiments in biological samples.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Khairallah RJ,Sadayappan Sdoi
10.1002/pmic.201300445subject
Has Abstractpub_date
2013-11-01 00:00:00pages
3245-6issue
22eissn
1615-9853issn
1615-9861journal_volume
13pub_type
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