Proteomic analysis of right and left cardiac ventricles under aerobic conditions and after ischemia/reperfusion.

abstract：:Previous studies have found that some first onset schizophrenia patients show signs of impaired insulin signaling. Also, epidemiological studies have shown that periods of suboptimal nutrition including protein deficiencies during pregnancy can lead to increased incidence of metabolic conditions and psychiatric disord...

journal_title：Proteomics

authors： Guest PC,Urday S,Ma D,Stelzhammer V,Harris LW,Amess B,Pietsch S,Oheim C,Ozanne SE,Bahn S

更新日期：2012-12-01 00:00:00

abstract：:Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous seru...

journal_title：Proteomics

authors： Bijttebier J,Tilleman K,Dhaenens M,Deforce D,Van Soom A,Maes D

更新日期：2009-10-01 00:00:00

abstract：:A brief period of ischemia followed by timely reperfusion may lead to prolonged, yet reversible, contractile dysfunction (myocardial stunning). Damage to the myocardium occurs not only during ischemia, but also during reperfusion, where a massive release of oxygen-free radicals (OFR) occurs. We have previously utilize...

journal_title：Proteomics

authors： White MY,Tchen AS,McCarron HC,Hambly BD,Jeremy RW,Cordwell SJ

更新日期：2006-12-01 00:00:00

abstract：:Until now, no data are available on the outer membrane (OM) proteome of Erwinia amylovora, a Gram-negative plant pathogen, causing fire blight in most of the members of the Rosaceae family. Since the OM forms the interface between the bacterial cell and its environment it is in direct contact with the host. Additional...

journal_title：Proteomics

authors： Holtappels M,Noben JP,Valcke R

更新日期：2016-09-01 00:00:00

abstract：:Isolated from Elephantopus scaber L., a Chinese medicinal herb that is widely used to prevent and treat cancers in China, isodeoxyelephantopin (ESI) exerted antitumor effects on several cancer cells. However, its antitumor mechanism is still not clear. In this study, we found that ESI could induce G2/M arrest and subs...

journal_title：Proteomics

authors： Yan GR,Tan Z,Wang Y,Xu ML,Yu G,Li Y,He QY

更新日期：2013-11-01 00:00:00

abstract：:Of all protein PTMs, glycosylation is by far the most common, and is a target for proteomic research. Glycosylation plays key roles in controlling various cellular processes and the modifications of the glycan structures in diseases highlight the clinical importance of this PTM. Glycosylation analysis remains a diffic...

journal_title：Proteomics

authors： Morelle W,Canis K,Chirat F,Faid V,Michalski JC

更新日期：2006-07-01 00:00:00

abstract：:Imbalance in protein homeostasis in specific subcellular organelles is alleviated through organelle-specific stress response pathways. As a canonical example of stress activated pathway, accumulation of misfolded proteins in ER activates unfolded protein response (UPR) in almost all eukaryotic organisms. However, very...

journal_title：Proteomics

authors： Maity S,Basak T,Bhat A,Bhasin N,Ghosh A,Chakraborty K,Sengupta S

更新日期：2014-08-01 00:00:00

abstract：:Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...

journal_title：Proteomics

authors： Liu K,Zhang J,Fu B,Xie H,Wang Y,Qian X

更新日期：2014-07-01 00:00:00

abstract：:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...

journal_title：Proteomics

authors： Corrigan L,Jefferies C,Clive Lee T,Daly J

更新日期：2011-08-01 00:00:00

abstract：:The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...

journal_title：Proteomics

authors： Bigeard J,Rayapuram N,Bonhomme L,Hirt H,Pflieger D

更新日期：2014-10-01 00:00:00

abstract：:Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...

journal_title：Proteomics

authors： Schuemie M,Chichester C,Lisacek F,Coute Y,Roes PJ,Sanchez JC,Kors J,Mons B

更新日期：2007-03-01 00:00:00

abstract：:Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional, immunological and structural aspects of both eukaryotic and prokaryotic glycoproteins. Traditional strategies used to analyse glycoprotein O-glycans involve glycoprotein isolation, followed by glycan release using solu...

journal_title：Proteomics

authors： Taylor AM,Holst O,Thomas-Oates J

更新日期：2006-05-01 00:00:00

abstract：:Depletion of abundant proteins is one of the effective ways to improve detection and identification of low-abundance proteins. Our previous study showed that protamine sulfate precipitation (PSP) method can deplete abundant ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from leaf proteins and is suitable fo...

journal_title：Proteomics

authors： Kim YJ,Wang Y,Gupta R,Kim SW,Min CW,Kim YC,Park KH,Agrawal GK,Rakwal R,Choung MG,Kang KY,Kim ST

更新日期：2015-05-01 00:00:00

abstract：:ProDaC (Proteomics Data Collection), a "Coordination Action" within the 6(th) EU framework programme, was created to support the collection, distribution and public availability of data from proteomics experiments. Within the consortium standards are created and maintained enabling an extensive data collection within ...

journal_title：Proteomics

authors： Eisenacher M,Kohl M,Martens L,Barsnes H,Hardt T,Levander F,Häkkinen J,Apweiler R,Meyer HE,Stephan C

更新日期：2009-01-01 00:00:00

abstract：:In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes ...

journal_title：Proteomics

authors： Laria AE,Messineo S,Arcidiacono B,Varano M,Chiefari E,Semple RK,Rocha N,Russo D,Cuda G,Gaspari M,Brunetti A,Foti DP

更新日期：2018-04-01 00:00:00

abstract：:Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatr...

journal_title：Proteomics

authors： Beasley CL,Pennington K,Behan A,Wait R,Dunn MJ,Cotter D

更新日期：2006-06-01 00:00:00

abstract：:This study describes the gel-free phosphoproteomic analysis of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination of strong cation exchange and IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing over ...

journal_title：Proteomics

authors： Davanture M,Dumur J,Bataillé-Simoneau N,Campion C,Valot B,Zivy M,Simoneau P,Fillinger S

更新日期：2014-07-01 00:00:00

abstract：:Advanced glycation endproduct (AGE) formation is an important mechanism for protein deterioration during diabetic complications and ageing. The effects on AGE formation following dihydroxyacetone (DHA) stress were studied in two model organisms, the yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegan...

journal_title：Proteomics

authors： Molin M,Pilon M,Blomberg A

更新日期：2007-10-01 00:00:00

abstract：:This study aimed at establishing a sensitive multiple reaction monitoring-mass spectrometry (MRM-MS) method for the quantification of the drug metabolizing cytochrome P450 (CYP)3A4 enzyme in human liver homogenates. Liver samples were subjected to trypsin digestion. MRM-MS analyses were performed using three transitio...

journal_title：Proteomics

authors： Cieślak A,Kelly I,Trottier J,Verreault M,Wunsch E,Milkiewicz P,Poirier G,Droit A,Barbier O

更新日期：2016-11-01 00:00:00

abstract：:In the post-genomic era, mass spectrometry is destined to fulfil a central role in biomedical research, and it is in the area of protein identification that mass spectrometry is now most rapidly expanding. An important identification method is to subject a protein to proteolysis and determine the resulting peptide mas...

journal_title：Proteomics

authors： Griffiths WJ,Jonsson AP

更新日期：2001-08-01 00:00:00

abstract：:Since detergent-resistant lipid rafts play important roles in the signal transduction for myogenesis, their comprehensive proteomic analysis could provide new insights to understand their function in myotubes. Here, the detergent-resistant lipid rafts were isolated from C2C12 myotubes and analyzed by capillary RPLC/MS...

journal_title：Proteomics

authors： Kim BW,Lee JW,Choo HJ,Lee CS,Jung SY,Yi JS,Ham YM,Lee JH,Hong J,Kang MJ,Chi SG,Hyung SW,Lee SW,Kim HM,Cho BR,Min DS,Yoon G,Ko YG

更新日期：2010-07-01 00:00:00

abstract：:Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after i...

journal_title：Proteomics

authors： Iwata K,Café-Mendes CC,Schmitt A,Steiner J,Manabe T,Matsuzaki H,Falkai P,Turck CW,Martins-de-Souza D

更新日期：2013-12-01 00:00:00

abstract：:Cancer research is striving toward new frontiers of assigning the correct personalized drug(s) to a given patient. However, extensive tumor heterogeneity poses a major obstacle. Tumors of the same type often respond differently to therapy, due to patient-specific molecular aberrations and/or untargeted tumor subpopula...

journal_title：Proteomics

authors： Kravchenko-Balasha N

更新日期：2020-07-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:While significant advances have been made in the diagnosis and treatment of prostate cancer, each year tens of thousands of men still die from prostate cancer in the United States. Thus, greater understanding of cellular pathways and molecular basis of prostate cancer progression in the development of androgen resista...

journal_title：Proteomics

authors： Höti N,Shah P,Hu Y,Yang S,Zhang H

更新日期：2017-03-01 00:00:00

abstract：:The G-protein complex is a cytoplasmic on-off molecular switch that is set by plasma membrane receptors that activate upon binding of its cognate extracellular agonist. In animals, the default setting is the "off" resting state, while in plants, the default state is constitutively "on" but repressed by a plasma membra...

journal_title：Proteomics

authors： Song G,Brachova L,Nikolau BJ,Jones AM,Walley JW

更新日期：2018-12-01 00:00:00

abstract：:Oligogalacturonides (OGs) are elicitors of plant defence responses released from the homogalacturonan of the plant cell wall during the attack by pathogenic micro-organisms. The signalling pathway mediated by OGs remains poorly understood, and no proteins involved in their signal perception and transduction have yet b...

journal_title：Proteomics

authors： Casasoli M,Spadoni S,Lilley KS,Cervone F,De Lorenzo G,Mattei B

更新日期：2008-03-01 00:00:00

abstract：:Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...

journal_title：Proteomics

authors： Gao Y,Lee H,Kwon OK,Cheng Z,Tan M,Kim KT,Lee S

更新日期：2019-05-01 00:00:00

abstract：:As an initial screen for novel markers of renal cancer and to minimise background heterogeneity, we have compared the within-patient profiles of serum samples from seven patients pre- and post-nephrectomy. Samples were depleted of six of the most abundant proteins using Agilent's multiple affinity removal system (MARS...

journal_title：Proteomics

authors： Vasudev NS,Ferguson RE,Cairns DA,Stanley AJ,Selby PJ,Banks RE

更新日期：2008-12-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00