Abstract:
:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Corrigan L,Jefferies C,Clive Lee T,Daly Jdoi
10.1002/pmic.201000587subject
Has Abstractpub_date
2011-08-01 00:00:00pages
3415-9issue
16eissn
1615-9853issn
1615-9861journal_volume
11pub_type
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