Direct visualization of serine hydrolase activities in complex proteomes using fluorescent active site-directed probes.

abstract：:Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...

journal_title：Proteomics

authors： Jackson CA,Yadav N,Min S,Li J,Milliman EJ,Qu J,Chen YC,Yu MC

更新日期：2012-11-01 00:00:00

abstract：:Depletion of abundant proteins is one of the effective ways to improve detection and identification of low-abundance proteins. Our previous study showed that protamine sulfate precipitation (PSP) method can deplete abundant ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from leaf proteins and is suitable fo...

journal_title：Proteomics

authors： Kim YJ,Wang Y,Gupta R,Kim SW,Min CW,Kim YC,Park KH,Agrawal GK,Rakwal R,Choung MG,Kang KY,Kim ST

更新日期：2015-05-01 00:00:00

abstract：:Staphylococcus aureus is an opportunistic human pathogen, which can cause life-threatening disease. Proteome analyses of the bacterium can provide new insights into its pathophysiology and important facets of metabolic adaptation and, thus, aid the recognition of targets for intervention. However, the value of such pr...

journal_title：Proteomics

authors： Depke M,Michalik S,Rabe A,Surmann K,Brinkmann L,Jehmlich N,Bernhardt J,Hecker M,Wollscheid B,Sun Z,Moritz RL,Völker U,Schmidt F

更新日期：2015-11-01 00:00:00

abstract：:Mammalian cells secrete two types of extracellular vesicles either constitutively or in a regulated manner: exosomes (50-100 nm in diameter) released from the intracellular compartment and ectosomes (also called microvesicles, 100-1000 nm in diameter) shed directly from the plasma membrane. Extracellular vesicles are ...

journal_title：Proteomics

authors： Choi DS,Kim DK,Kim YK,Gho YS

更新日期：2013-05-01 00:00:00

abstract：:Secreted proteins play a pivotal role in cellular functions. To better understand malignant behavior, we adapted stable isotopic labeling with amino acids in cell culture technology to identify and quantify proteins differentially released into the extracellular media by a pair of normal and malignant breast-cancer ce...

journal_title：Proteomics

authors： Liang X,Huuskonen J,Hajivandi M,Manzanedo R,Predki P,Amshey JR,Pope RM

更新日期：2009-01-01 00:00:00

abstract：:Mechanisms of milk lipid secretion are highly controversial. Analyzing the fine protein composition of the "milk fat globule membrane" (MFGM), the triple-layered membrane surrounding milk lipid droplets (LDs) can provide mechanistic clues to better understand LD biosynthesis and secretion pathways in mammary epithelia...

journal_title：Proteomics

authors： Henry C,Saadaoui B,Bouvier F,Cebo C

更新日期：2015-07-01 00:00:00

abstract：:Omic technologies have enabled the complete readout of the molecular state of a cell at different biological scales. In principle, the combination of multiple omic data types can provide an integrated view of the entire biological system. This integration requires appropriate models in a systems biology approach. Here...

journal_title：Proteomics

authors： Dahal S,Yurkovich JT,Xu H,Palsson BO,Yang L

更新日期：2020-09-01 00:00:00

abstract：:The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...

journal_title：Proteomics

authors： Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

更新日期：2012-06-01 00:00:00

abstract：:Protein arginine methyltransferase 5 (PRMT5) is a major enzyme responsible for generating monomethyl and symmetric dimethyl arginine in proteins. PRMT5 is essential for cell viability and development, and its overexpression is observed in a variety of cancers. In the present study, it is found that levels of PRMT5 pro...

journal_title：Proteomics

authors： Lim Y,Park YE,Ha S,Lee JE,Kim HC

更新日期：2020-05-28 00:00:00

abstract：:The identification of specific protein markers for colorectal cancer would provide the basis for early diagnosis and detection, as well as clues for understanding the molecular mechanisms governing cancer progression. In this report, we describe the proteomic analysis of the samples of colorectal cancer corresponding ...

journal_title：Proteomics

authors： Alfonso P,Núñez A,Madoz-Gurpide J,Lombardia L,Sánchez L,Casal JI

更新日期：2005-07-01 00:00:00

abstract：:Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...

journal_title：Proteomics

authors： Guergues J,Zhang P,Liu B,Stevens SM Jr

更新日期：2019-06-01 00:00:00

abstract：:In the era of quantitative proteomics, where mass spectrometry plays a pivotal role, in particular associated with the use of data-independent acquisition, it is time to perform an overview of this growing field with special focus on one of the most promising approaches: SWATH-MS, and to present future perspectives fo...

journal_title：Proteomics

authors： Anjo SI,Santa C,Manadas B

更新日期：2017-02-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are implicated in a range of human diseases, some of which are associated with the ability to bind to lipids. Although the presence of solvent-exposed hydrophobic regions in IDPs should favor their interactions with low-molecular-weight hydrophobic/amphiphilic compounds, this h...

journal_title：Proteomics

authors： Deryusheva E,Nemashkalova E,Galloux M,Richard CA,Eléouët JF,Kovacs D,Van Belle K,Tompa P,Uversky V,Permyakov S

更新日期：2019-03-01 00:00:00

abstract：:Aspergillus fumigatus faces abrupt changes in oxygen concentrations at the site of infection. An increasing number of studies has demonstrated that elevated production of intracellular reactive oxygen species (ROS) under low oxygen conditions plays a regulatory role in modulating cellular responses for adaptation to h...

journal_title：Proteomics

authors： Shekhova E,Ivanova L,Krüger T,Stroe MC,Macheleidt J,Kniemeyer O,Brakhage AA

更新日期：2019-03-01 00:00:00

abstract：:Proteolytic modification of components of the extracellular milieu by metalloproteinases plays important roles in the regulation of multiple cellular and physiological processes and pathological conditions. ADAMTS1 is a secreted enzyme of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) fami...

journal_title：Proteomics

authors： Canals F,Colomé N,Ferrer C,Plaza-Calonge Mdel C,Rodríguez-Manzaneque JC

更新日期：2006-04-01 00:00:00

abstract：:Human butyrophilin (BTN) expression in milk fat globule (MFGM) was evaluated using two dimensional electrophoresis (2-DE) as the separation technique, and peptide mass mapping by matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) as the identification tool. Since milk composition changes througho...

journal_title：Proteomics

authors： Cavaletto M,Giuffrida MG,Fortunato D,Gardano L,Dellavalle G,Napolitano L,Giunta C,Bertino E,Fabris C,Conti A

更新日期：2002-07-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. Miniaturization of IMAC column is essential for the analysis of a small amount of sample. Nanoscale IMAC column was prepared by chemical modification of...

journal_title：Proteomics

authors： Feng S,Pan C,Jiang X,Xu S,Zhou H,Ye M,Zou H

更新日期：2007-02-01 00:00:00

abstract：:Heat shock proteins (HSP) are families of highly conserved proteins which are induced in cells and tissues upon exposure to extreme conditions causing acute or chronic stress. They exhibit distinct functions and have been implicated in the pathogenesis of a number of diseases, including cancer. A causal relationship b...

journal_title：Proteomics

authors： Lichtenfels R,Kellner R,Bukur J,Beck J,Brenner W,Ackermann A,Seliger B

更新日期：2002-05-01 00:00:00

abstract：:We present here the first proteomics analysis of uveal melanoma (UM) cells. These cells represent a good model for the identification of polypeptide markers, which could be developed as diagnostic tools. UM is the most common primary intraocular tumour in adults. In contrast to other cancers, the survival rate of pati...

journal_title：Proteomics

authors： Pardo M,García A,Thomas B,Piñeiro A,Akoulitchev A,Dwek RA,Zitzmann N

更新日期：2005-12-01 00:00:00

abstract：:Gene expression analyses of embryonic stem cells (ESCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal and pluripotency. We employed a 2-DE-based proteomics approach to analyze human ESC line, Royan H5, in undifferentiated cells and differ...

journal_title：Proteomics

authors： Fathi A,Pakzad M,Taei A,Brink TC,Pirhaji L,Ruiz G,Sharif Tabe Bordbar M,Gourabi H,Adjaye J,Baharvand H,Salekdeh GH

更新日期：2009-11-01 00:00:00

abstract：:Exercise training has been recommended as a nonpharmacological strategy for the prevention and attenuation of skeletal muscle atrophy in distinct pathophysiological conditions. Despite the well-established phenotypic alterations, the molecular mechanisms underlying exercise-induced skeletal muscle remodeling are poorl...

journal_title：Proteomics

authors： Padrão AI,Ferreira R,Amado F,Vitorino R,Duarte JA

更新日期：2016-03-01 00:00:00

abstract：:Malt derived from barley malting is an essential raw material for beer brewing. In this study, we performed the first dynamic proteome survey during barley malting using a gel-free proteomics approach. This entailed in-solution tryptic digestion of precipitated proteins and analysis of peptides by nanoliquid chromatog...

journal_title：Proteomics

authors： Mahalingam R

更新日期：2018-08-01 00:00:00

abstract：:Following transmission to the vertebrate host, the protozoan parasite Leishmania donovani differentiates into the pathogenic amastigote stage that is adapted for intracellular survival. This developmental transition is induced by environmental factors including elevated temperature and acidic pH and is likely transduc...

journal_title：Proteomics

authors： Morales MA,Watanabe R,Laurent C,Lenormand P,Rousselle JC,Namane A,Späth GF

更新日期：2008-01-01 00:00:00

abstract：:Menthol is a naturally occurring cyclic monoterpene used in oral hygiene products, confectionary, pharmaceuticals, cosmetics, pesticides, and as a flavoring agent. In the present study, we analyzed the differentially expressing proteome in L-menthol-treated Caco-2 cell line as it was found to inhibit cell proliferatio...

journal_title：Proteomics

authors： Faridi U,Sisodia BS,Shukla AK,Shukla RK,Darokar MP,Dwivedi UN,Shasany AK

更新日期：2011-05-01 00:00:00

abstract：:The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...

journal_title：Proteomics

authors： Schaumburg J,Diekmann O,Hagendorff P,Bergmann S,Rohde M,Hammerschmidt S,Jänsch L,Wehland J,Kärst U

更新日期：2004-10-01 00:00:00

abstract：:This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. T...

journal_title：Proteomics

authors： Heo SH,Choi JH,Kim YM,Jung CW,Lee J,Jin HY,Kim GH,Lee BH,Shin CH,Yoo HW

更新日期：2013-04-01 00:00:00

abstract：:Mulberry dwarf (MD) is a serious infectious disease of mulberry caused by phytoplasma. Infection with MD phytoplasma results in stress phenotypes of yellowing, phyllody, stunting, proliferation, and witches' broom. Physiological and biochemical analysis has shown that infection with MD phytoplasma causes an increase i...

journal_title：Proteomics

authors： Ji X,Gai Y,Zheng C,Mu Z

更新日期：2009-12-01 00:00:00

abstract：:To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by microLC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene ex...

journal_title：Proteomics

authors： Bernardini G,Arena S,Braconi D,Scaloni A,Santucci A

更新日期：2007-09-01 00:00:00

abstract：:The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...

journal_title：Proteomics

authors： Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

更新日期：2019-11-01 00:00:00

abstract：:Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...

journal_title：Proteomics

authors： Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

更新日期：2011-04-01 00:00:00