Addressing the Challenges of High-Throughput Cancer Tissue Proteomics for Clinical Application: ProCan.

abstract：:Two-dimensional difference gel electrophoresis (2-D DIGE) coupled with mass spectrometry (MS) was used to investigate tumor-specific changes in the proteome of human colorectal cancers and adjacent normal mucosa. For each of six patients with different stages of colon cancer, Cy5-labeled proteins isolated from tumor t...

journal_title：Proteomics

authors： Friedman DB,Hill S,Keller JW,Merchant NB,Levy SE,Coffey RJ,Caprioli RM

更新日期：2004-03-01 00:00:00

abstract：:In this article, we provide a comprehensive study of the content of the Universal Protein Resource (UniProt) protein data sets for human and mouse. The tryptic search spaces of the UniProtKB (UniProt knowledgebase) complete proteome sets were compared with other data sets from UniProtKB and with the corresponding Inte...

journal_title：Proteomics

authors： Alpi E,Griss J,da Silva AW,Bely B,Antunes R,Zellner H,Ríos D,O'Donovan C,Vizcaíno JA,Martin MJ

更新日期：2015-01-01 00:00:00

abstract：:In mass spectrometry-based proteomics, protein identification results usually consist of peptide sequences and database-dependent accession identifiers of the matching proteins. Often certain annotations are only available in particular databases that in turn must be queried by a certain identifier. In order to simpli...

journal_title：Proteomics

authors： Boehm AM,Sickmann A

更新日期：2006-08-01 00:00:00

abstract：:Collagen-related peptide (CRP) stimulates powerful activation of platelets through the glycoprotein VI (GPVI)-FcR gamma-chain complex. We have combined proteomics and traditional biochemistry approaches to study the proteome of CRP-activated platelets, focusing in detail on tyrosine phosphorylation. In two separate ap...

journal_title：Proteomics

authors： García A,Senis YA,Antrobus R,Hughes CE,Dwek RA,Watson SP,Zitzmann N

更新日期：2006-10-01 00:00:00

abstract：:Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...

journal_title：Proteomics

authors： Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

更新日期：2005-04-01 00:00:00

abstract：:Among the group of lactic acid bacteria, Streptococcus thermophilus has found a wide application in industrial processes used for the manufacture of dairy products. Taking advantage of different proteome extraction and subfractionation protocols, bacterial cytosolic and membrane proteins were isolated and resolved by ...

journal_title：Proteomics

authors： Salzano AM,Arena S,Renzone G,D'Ambrosio C,Rullo R,Bruschi M,Ledda L,Maglione G,Candiano G,Ferrara L,Scaloni A

更新日期：2007-05-01 00:00:00

abstract：:A method is introduced to evaluate protein concentrations using the height sum of all MALDI-MS peaks that unambiguously match theoretic tryptic peptide masses of the protein sought after. The method uses native chromatographic protein fractionation prior to digestion but does not require any depletion, labeling, deriv...

journal_title：Proteomics

authors： Bublitz R,Kreusch S,Ditze G,Schulze M,Cumme GA,Fischer C,Winter A,Hoppe H,Rhode H

更新日期：2006-07-01 00:00:00

abstract：:The G-protein complex is a cytoplasmic on-off molecular switch that is set by plasma membrane receptors that activate upon binding of its cognate extracellular agonist. In animals, the default setting is the "off" resting state, while in plants, the default state is constitutively "on" but repressed by a plasma membra...

journal_title：Proteomics

authors： Song G,Brachova L,Nikolau BJ,Jones AM,Walley JW

更新日期：2018-12-01 00:00:00

abstract：:Deciphering a complete landscape of protein-RNA interactions in the human proteome remains an elusive challenge. We computationally elucidate RNA binding proteins (RBPs) using an approach that complements previous efforts. We employ two modern complementary sequence-based methods that provide accurate predictions from...

journal_title：Proteomics

authors： Chowdhury S,Zhang J,Kurgan L

更新日期：2018-11-01 00:00:00

abstract：:ProDaC (Proteomics Data Collection), a "Coordination Action" within the 6(th) EU framework programme, was created to support the collection, distribution and public availability of data from proteomics experiments. Within the consortium standards are created and maintained enabling an extensive data collection within ...

journal_title：Proteomics

authors： Eisenacher M,Kohl M,Martens L,Barsnes H,Hardt T,Levander F,Häkkinen J,Apweiler R,Meyer HE,Stephan C

更新日期：2009-01-01 00:00:00

abstract：:Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

journal_title：Proteomics

authors： Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

更新日期：2006-02-01 00:00:00

abstract：:Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...

journal_title：Proteomics

authors： Kim SY,Joglekar MV,Hardikar AA,Phan TH,Khanal D,Tharkar P,Limantoro C,Johnson J,Kalionis B,Chrzanowski W

更新日期：2019-09-01 00:00:00

abstract：:Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due ...

journal_title：Proteomics

authors： Yuan C,Jiao L,Yang L,Ying W,Hu Z,Liu J,Cui F,Li L,Qian L,Teng Y,Hang H,Qian X,Yang X

更新日期：2008-06-01 00:00:00

abstract：:The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...

journal_title：Proteomics

authors： Yamamoto T,Langham RG,Ronco P,Knepper MA,Thongboonkerd V

更新日期：2008-06-01 00:00:00

abstract：:Suberoylanilide hydroxamic acid (SAHA) is a potent histone deacetylase (HDAC) inhibitor. Inhibitors of HDACs are used in cancer therapy based on the role HDACs play in transcription by regulating chromatin compaction and non-histone proteins such as transcription factors. Profiling of HDAC expression is of interest in...

journal_title：Proteomics

authors： Fischer JJ,Michaelis S,Schrey AK,Diehl A,Graebner OY,Ungewiss J,Horzowski S,Glinski M,Kroll F,Dreger M,Koester H

更新日期：2011-10-01 00:00:00

abstract：:Neurons present a highly polarized morphology, often displaying a significantly imbalanced distribution of the cytoplasm between the somatic and axonal domains. This imbalance requires cell-specific mechanisms for the maintenance of the axoplasmic mass during development, neuronal homeostasis, and recovery after injur...

journal_title：Proteomics

authors： Farias J,Sotelo JR,Sotelo-Silveira J

更新日期：2019-08-01 00:00:00

abstract：:Protein-ligand interactions serve as fundamental regulators of numerous biological processes. Among protein-ligand pairs, glycan binding proteins (GBPs) and the glycans they recognize represent unique and highly complex interactions implicated in a broad range of regulatory activities. With few exceptions, cell surfac...

journal_title：Proteomics

authors： Kamili NA,Arthur CM,Gerner-Smidt C,Tafesse E,Blenda A,Dias-Baruffi M,Stowell SR

更新日期：2016-12-01 00:00:00

abstract：:In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator(®) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated ...

journal_title：Proteomics

authors： Yang X,Levin Y,Rahmoune H,Ma D,Schöffmann S,Umrania Y,Guest PC,Bahn S

更新日期：2011-02-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:Intrinsic or acquired resistance to vincristine (VCR), an antimicrotubule agent used in the treatment of childhood acute lymphoblastic leukemia (ALL), is a major clinical problem. Using a clinically relevant NOD/SCID mouse xenograft model of ALL, we established that alterations in the actin and tubulin cytoskeleton ar...

journal_title：Proteomics

authors： Verrills NM,Liem NL,Liaw TY,Hood BD,Lock RB,Kavallaris M

更新日期：2006-03-01 00:00:00

abstract：:It becomes increasingly clear that separation of pure cell populations provides a uniquely sensitive and accurate approach to protein profiling in biological systems and opens up a new area for proteomic analysis. The method we described could simultaneously isolate population of hepatocytes (HCs), hepatic stellate ce...

journal_title：Proteomics

authors： Liu W,Hou Y,Chen H,Wei H,Lin W,Li J,Zhang M,He F,Jiang Y

更新日期：2011-09-01 00:00:00

abstract：:Of all protein PTMs, glycosylation is by far the most common, and is a target for proteomic research. Glycosylation plays key roles in controlling various cellular processes and the modifications of the glycan structures in diseases highlight the clinical importance of this PTM. Glycosylation analysis remains a diffic...

journal_title：Proteomics

authors： Morelle W,Canis K,Chirat F,Faid V,Michalski JC

更新日期：2006-07-01 00:00:00

abstract：:MALDI-MS is an extremely flexible technique and can be synergistically used in conjunction with established bioanalytical methods such as PAGE or SPR. To that end, slight modifications on the sample target plate may be necessary. Those can involve the use of hydrophobic coatings for improved sample deposition and desa...

journal_title：Proteomics

authors： König S

更新日期：2008-02-01 00:00:00

abstract：:Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...

journal_title：Proteomics

authors： Allal AS,Kähne T,Reverdin AK,Lippert H,Schlegel W,Reymond MA

更新日期：2004-08-01 00:00:00

abstract：:Caulobacter crescentus, a Gram negative alpha-purple bacterium that displays an invariant asymmetric cell division pattern, has become a key model system for the study of bacterial development. Membrane proteins play key roles in cell cycle events, both as components of landmark morphological structures and as critica...

journal_title：Proteomics

authors： Phadke ND,Molloy MP,Steinhoff SA,Ulintz PJ,Andrews PC,Maddock JR

更新日期：2001-05-01 00:00:00

abstract：:Low root temperature causes a decrease in water uptake, which leads to mineral and nutrient deficiencies with potentially decreased root and shoot growth. Differential temperature effects in plants have been studied extensively, however, the effect of root chilling on the global protein expression in shoots has not be...

journal_title：Proteomics

authors： Neilson KA,Scafaro AP,Chick JM,George IS,Van Sluyter SC,Gygi SP,Atwell BJ,Haynes PA

更新日期：2013-06-01 00:00:00

abstract：:Protein complexes carry out almost the entire signaling and functional processes in the cell. The protein complex complement of a cell, and its network of complex-complex interactions, is referred to here as the complexome. Computational methods to predict protein complexes from proteomics data, resulting in network r...

journal_title：Proteomics

authors： Clancy T,Hovig E

更新日期：2014-01-01 00:00:00

abstract：:Mitochondria consist of four compartments, outer membrane, intermembrane space, inner membrane, and matrix; each harboring specific functions and structures. In this study, we used LC-MS/MS to characterize the protein composition of Trypanosoma brucei mitochondrial (mt) membranes, which were enriched by different bioc...

journal_title：Proteomics

authors： Acestor N,Panigrahi AK,Ogata Y,Anupama A,Stuart KD

更新日期：2009-12-01 00:00:00

abstract：:The main goal of this study was to analyze, using proteomic techniques, changes in protein expression of acute myeloid leukemia (AML) cells that could give insights into a better early prognosis for tumor pathophysiology. Proteomic analysis of different subtypes of AML cells was carried out using 2-DE and MALDI-TOF PM...

journal_title：Proteomics

authors： López-Pedrera C,Villalba JM,Siendones E,Barbarroja N,Gómez-Díaz C,Rodríguez-Ariza A,Buendía P,Torres A,Velasco F

更新日期：2006-04-01 00:00:00

abstract：:Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...

journal_title：Proteomics

authors： Beaudette P,Popp O,Dittmar G

更新日期：2016-01-01 00:00:00