Abstract:
:Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations of the technique, irrelevant results might be trusted. To address this issue, we here propose a step-by-step quality control of the iTRAQ workflow. From sample preparation to final ratio calculation we provide metrics and techniques assessing the actual effectiveness of iTRAQ quantification as well as a novel method for more reliable protein ratio estimation.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Burkhart JM,Vaudel M,Zahedi RP,Martens L,Sickmann Adoi
10.1002/pmic.201000711subject
Has Abstractpub_date
2011-03-01 00:00:00pages
1125-34issue
6eissn
1615-9853issn
1615-9861journal_volume
11pub_type
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