Abstract:
:A multiplexed fluorescence immunoassay using a novel planar waveguide technology-based microarray system, ZeptoMARK (Zeptosens), was developed to detect simultaneously urokinase-type plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1), and vascular endothelial growth factor (VEGF) in extracts of breast cancer tissues. The three analytes assay was cross-validated with single-analyte ELISA/chemiluminescence immunosorbent assay tests, revealing good correlations and enhanced assay sensitivities (LODs) of 1 pg/mL for uPA, 33 pg/mL for PAI-1, and 1 pg/mL for VEGF. Values were well within the 80-120% limits for assay recovery and within the +/-20% limits for assay precision. The uPA, PAI-1, and VEGF results obtained from 50 breast cancer cytosols using the protein array system demonstrated that the microarray-based multiplexed assay is a sensitive and robust tool to be used for the simultaneous quantification of cancer markers in small breast cancer tissue samples (core biopsies). The miniaturized, multiplexed assay format has a potential to be used for the quantitative analysis of a larger set of validated markers with significance in disease management.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Weissenstein U,Schneider MJ,Pawlak M,Cicenas J,Eppenberger-Castori S,Oroszlan P,Ehret S,Geurts-Moespot A,Sweep FC,Eppenberger Udoi
10.1002/pmic.200500078subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1427-36issue
5eissn
1615-9853issn
1615-9861journal_volume
6pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Among the group of lactic acid bacteria, Streptococcus thermophilus has found a wide application in industrial processes used for the manufacture of dairy products. Taking advantage of different proteome extraction and subfractionation protocols, bacterial cytosolic and membrane proteins were isolated and resolved by ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200601030
更新日期:2007-05-01 00:00:00
abstract::With the human Plasma Proteome Project (PPP) pilot phase completed, the largest and most ambitious proteomics experiment to date has reached its first milestone. The correspondingly impressive amount of data that came from this pilot project emphasized the need for a centralized dissemination mechanism and led to the ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401302
更新日期:2005-08-01 00:00:00
abstract::Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600693
更新日期:2007-03-01 00:00:00
abstract::Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either (13) C-naphthalene or (13) C-fluorene ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200569
更新日期:2013-10-01 00:00:00
abstract::Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800411
更新日期:2020-03-01 00:00:00
abstract::The activation of caspases is a critical event for the execution phase of programmed cell death. Caspases are highly specific in their ability to activate or inhibit many crucial proteins in the cell via site-specific cleavage. To date, more than 60 proteins have been shown to be substrates of one or more caspases in ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400979
更新日期:2004-11-01 00:00:00
abstract::Separation and identification of hydrophobic membrane proteins is a major challenge in proteomics. Identification of such sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins by peptide mass fingerprinting (PMF) via matrix-assisted laser desorption/ionization-time of flight (MALDI-TO...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400851
更新日期:2004-12-01 00:00:00
abstract::The International Plant Proteomics Organization (INPPO) is a global platform of the plant proteomics community or, more generally, the scientific community that uses proteomics to address plant biology. Organizing an international conference is one of its initiatives to promote plant proteomics by involving and gather...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.201570084
更新日期:2015-05-01 00:00:00
abstract::Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200356
更新日期:2013-03-01 00:00:00
abstract::As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600722
更新日期:2007-04-01 00:00:00
abstract::Two-dimensional electrophoresis is a widely used method for separating a large number of proteins from complex protein mixtures and for revealing differential patterns of protein expressions. In the computer-assisted proteome research, the comparison of protein separation profiles involves several heuristic steps, ran...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200211)2:11<1504::AID-PROT1504>3
更新日期:2002-11-01 00:00:00
abstract::Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401055
更新日期:2005-04-01 00:00:00
abstract::Unconjugated bilirubin (UCB) is a powerful antioxidant and a modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop a physiological jaundice, in case of severe hyperbilirubinemia UCB may become neurotoxic causing severe long-term neuronal damages, also kno...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900579
更新日期:2010-04-01 00:00:00
abstract::A classical proteomic analysis was used to establish a reference map of proteins associated with healthy human erythrocyte ghosts. Following osmotic lysis and differential centrifugation, ghost proteins were separated by either one-dimensional gel electrophoresis (1-DE) or two-dimensional gel electrophoresis (2-DE). S...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200209)2:9<1229::AID-PROT1229>3.
更新日期:2002-09-01 00:00:00
abstract::We present here an effective technique for the large-scale separation and identification of N-linked glycoproteins from Chang liver cells, the human normal liver cells. To enrich N-linked glycoproteins from the whole cells, a procedure containing the lysis of human liver cells, the solubilization of total proteins, le...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600041
更新日期:2007-07-01 00:00:00
abstract::Calcineurin is a heterodimeric serine/threonine protein phosphatase, important for many cellular processes such as T-cell regulation, cardiac hypertrophy and kidney development. We previously reported the characterization of Caenorhabditis elegans calcineurin mutants as providing a simple but excellent genetic model s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500315
更新日期:2006-02-01 00:00:00
abstract::We here present a user-friendly and extremely lightweight tool that can serve as a stand-alone front-end for the Open MS Search Algorithm (OMSSA) search engine, or that can directly be used as part of an informatics processing pipeline for MS driven proteomics. The OMSSA graphical user interface (OMSSAGUI) tool is wri...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701126
更新日期:2008-06-01 00:00:00
abstract::Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700461
更新日期:2018-05-01 00:00:00
abstract::Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200027
更新日期:2012-06-01 00:00:00
abstract::Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this study, the complementar...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800200
更新日期:2018-12-01 00:00:00
abstract::Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800266
更新日期:2018-09-01 00:00:00
abstract::Targeted mass spectrometry-based proteomics approaches enable the simultaneous and reproducible quantification of multiple protein analytes across numerous conditions in biology and clinical studies. These approaches involve e.g. selected reaction monitoring (SRM) typically conducted on a triple quadrupole mass spectr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600323
更新日期:2017-03-01 00:00:00
abstract::Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300201
更新日期:2013-12-01 00:00:00
abstract::In this study we investigated the cellular response to the application of high hydrostatic pressure. High pressure is increasingly used for food preservation. With high resolution 2-D electrophoresis we compared the protein patterns of atmospherically grown Lactobacillus sanfranciscensis with those pressure treated up...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200206)2:6<765::AID-PROT765>3.0.
更新日期:2002-06-01 00:00:00
abstract::With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201400373
更新日期:2015-03-01 00:00:00
abstract::Rice is susceptible to cold stress and with a future of climatic instability we will be unable to produce enough rice to satisfy increasing demand. A thorough understanding of the molecular responses to thermal stress is imperative for engineering cultivars, which have greater resistance to low temperature stress. In ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000727
更新日期:2011-05-01 00:00:00
abstract::Autosomal dominant tubulointerstitial kidney disease associated to the MUC1 gene (ADTKD-MUC1; formerly MCKD1) belongs to a heterogeneous group of rare hereditary kidney diseases that is prototypically caused by frameshift mutations in the MUC1 repeat domain. The mutant MUC1 (insC) lacks the transmembrane domaine, exhi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700456
更新日期:2018-04-01 00:00:00
abstract::High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200390026
更新日期:2003-02-01 00:00:00
abstract::The spring workshop of the HUPO-PSI convened in Siena to further progress the data standards which are already making an impact on data exchange and deposition in the field of proteomics. Separate work groups pushed forward existing XML standards for the exchange of Molecular Interaction data (PSI-MI, MIF) and Mass Sp...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200500626
更新日期:2005-09-01 00:00:00
abstract::Cells are a fundamental unit of life, and the ability to study the phenotypes and behaviors of individual cells is crucial to understanding the workings of complex biological systems. Cell phenotypes (epigenomic, transcriptomic, proteomic, and metabolomic) exhibit dramatic heterogeneity between and within the differen...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201700312
更新日期:2018-09-01 00:00:00