A global proteomics approach identifies novel phosphorylated signaling proteins in GPVI-activated platelets: involvement of G6f, a novel platelet Grb2-binding membrane adapter.

abstract：:The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...

journal_title：Proteomics

authors： Lubelwana Hafver T,Wanichawan P,Manfra O,de Souza GA,Lunde M,Martinsen M,Louch WE,Sejersted OM,Carlson CR

更新日期：2017-09-01 00:00:00

abstract：:Phosphoproteomics can be defined as the comprehensive study of protein phosphorylation by identification of the phosphoproteins, exact mapping of the phosphorylation sites, quantification of phosphorylation, and eventually, revealing their biological function. Its place in today's research is vitally important to addr...

journal_title：Proteomics

authors： Kersten B,Agrawal GK,Iwahashi H,Rakwal R

更新日期：2006-10-01 00:00:00

abstract：:Myostatin plays a major role in muscle growth and development and animals with disruption of this gene display marked increases in muscle mass. Little is known about muscle physiological adaptations in relation to this muscle hypertrophy. To provide a more comprehensive view, we analyzed bovine muscles from control, h...

journal_title：Proteomics

authors： Bouley J,Meunier B,Chambon C,De Smet S,Hocquette JF,Picard B

更新日期：2005-02-01 00:00:00

abstract：:A curated set of 81 proven and 44 predicted lipoproteins of Escherichia coli K-12 was defined with the combined use of a literature survey, a variety of predictive tools and human expertise. The well-documented Gram-negative proteome of E. coli K-12 was chosen to assess how the different approaches complement each oth...

journal_title：Proteomics

authors： Gonnet P,Rudd KE,Lisacek F

更新日期：2004-06-01 00:00:00

abstract：:The aims of the study were to: (i) identify differentially regulated proteins in cerebrospinal fluid (CSF) between multiple sclerosis (MS) patients and non-MS controls; (ii) examine the effect of matching the CSF samples on either total protein amount or volume, and compare four protein normalization strategies for CS...

journal_title：Proteomics

authors： Opsahl JA,Vaudel M,Guldbrandsen A,Aasebø E,Van Pesch V,Franciotta D,Myhr KM,Barsnes H,Berle M,Torkildsen Ø,Kroksveen AC,Berven FS

更新日期：2016-04-01 00:00:00

abstract：:Heat shock proteins (HSPs) are crucial for the maintenance of cell integrity during normal cellular growth as well as during pathophysiological conditions. While functioning mainly as molecular chaperones, HSPs also appear to be involved in diverse biological activities, such as apoptosis, carcinogenesis, and cytoprot...

journal_title：Proteomics

authors： Yeo M,Park HK,Kim DK,Cho SW,Kim YS,Cho SY,Paik YK,Hahm KB

更新日期：2004-11-01 00:00:00

abstract：:The ability to obtain the accurate mass of a protein in a complex sample mixture aids in determining its correct in vivo form. This is important when identifying post-translationally modified proteins, protein variants or isoforms. The central technique used to separate proteins, 2-dimensional gel electrophoresis offe...

journal_title：Proteomics

authors： Fung KY,Askovic S,Basile F,Duncan MW

更新日期：2004-10-01 00:00:00

abstract：:There is ample evidence for the involvement of protein phosphorylation on serine/threonine/tyrosine in bacterial signaling and regulation, but very few exact phosphorylation sites have been experimentally determined. Recently, gel-free high accuracy MS studies reported over 150 phosphorylation sites in two bacterial m...

journal_title：Proteomics

authors： Miller ML,Soufi B,Jers C,Blom N,Macek B,Mijakovic I

更新日期：2009-01-01 00:00:00

abstract：:Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry-based proteomics. Their common principle is to comprehensively measur...

journal_title：Proteomics

authors： Mann M

更新日期：2020-08-03 00:00:00

abstract：:Renal cell carcinoma (RCC) originates in the renal cortex. It accounts for 2-3 percent of all cancers occurring in adults and it is characterised by lack of early clinical manifestations, unpredictable outcome, and absence of effective treatment modalities except early surgery. RCC comprises a heterogeneous group of t...

journal_title：Proteomics

authors： Sarto C,Déon C,Doro G,Hochstrasser DF,Mocarelli P,Sanchez JC

更新日期：2001-10-01 00:00:00

abstract：:The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...

journal_title：Proteomics

authors： Rahman MA,Karl K,Nonaka M,Fujimura H,Shinjo R,Oomori T,Wörheide G

更新日期：2014-11-01 00:00:00

abstract：:The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...

journal_title：Proteomics

authors： Blair BG,Wu X,Zahari MS,Mohseni M,Cidado J,Wong HY,Beaver JA,Cochran RL,Zabransky DJ,Croessmann S,Chu D,Toro PV,Cravero K,Pandey A,Park BH

更新日期：2015-01-01 00:00:00

abstract：:SARS-CoV-2 infection results in a mild-to-moderate disease course in most patients, allowing outpatient self-care and quarantine. However, in approx. 10% of cases a two- or three-phasic critical disease course with starting from day 7 to 10 is observed. To facilitate and plan outpatient care, biomarkers prognosing suc...

journal_title：Proteomics

authors： Wendt R,Kalbitz S,Lübbert C,Kellner N,Macholz M,Schroth S,Ermisch J,Latosisnka A,Arnold B,Mischak H,Beige J,Metzger J

更新日期：2020-09-10 00:00:00

abstract：:Ruminants make large contributions to sustainable agriculture by converting crop by-products into agricultural food. Multi-omics integrative analysis helps to uncover the underlying molecular mechanisms. The liver metabolome-transcriptome interface (LMTI) in dairy cows, including 3938 significant correlations (p < 0.0...

journal_title：Proteomics

authors： Sun HZ,Wang DM,Liu HY,Liu JX

更新日期：2018-08-01 00:00:00

abstract：:Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...

journal_title：Proteomics

authors： Castagna A,Antonioli P,Astner H,Hamdan M,Righetti SC,Perego P,Zunino F,Righetti PG

更新日期：2004-10-01 00:00:00

abstract：:Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zin...

journal_title：Proteomics

authors： Irmak S,Tilki D,Heukeshoven J,Oliveira-Ferrer L,Friedrich M,Huland H,Ergün S

更新日期：2005-11-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertili...

journal_title：Proteomics

authors： Oliva R,de Mateo S,Estanyol JM

更新日期：2009-02-01 00:00:00

abstract：:Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multi...

journal_title：Proteomics

authors： Moretti M,Grunau A,Minerdi D,Gehrig P,Roschitzki B,Eberl L,Garibaldi A,Gullino ML,Riedel K

更新日期：2010-09-01 00:00:00

abstract：:In-gel peptide digestion has become a widely used technique for characterizing proteins resolved by two-dimensional gel electrophoresis. Peptides generated from gel pieces are frequently contaminated with detergent and salts. Prior to matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysi...

journal_title：Proteomics

authors： Pluskal MG,Bogdanova A,Lopez M,Gutierrez S,Pitt AM

更新日期：2002-02-01 00:00:00

abstract：:The CpG motif of bacterial DNA (CpG-DNA) is a potent immunostimulating agent whose mechanism of action is not yet clear. Here, we used both DNA microarray and proteomic approaches to investigate the effects of oligodeoxynucleotides containing the CpG motif (CpG-ODN) on gene transcription and protein expression profile...

journal_title：Proteomics

authors： Kuo CC,Kuo CW,Liang CM,Liang SM

更新日期：2005-03-01 00:00:00

abstract：:Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...

journal_title：Proteomics

authors： Finehout EJ,Cantor JR,Lee KH

更新日期：2005-06-01 00:00:00

abstract：:Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs)...

journal_title：Proteomics

authors： Evans J,Rai A,Nguyen HPT,Poh QH,Elglass K,Simpson RJ,Salamonsen LA,Greening DW

更新日期：2019-12-01 00:00:00

abstract：:In this study, poly(glycidyl methacrylate-ethyleneglycol dimethacrylate) monolith functionalized with cobalt phthalocyanine tetracarboxylic acid is prepared. The polymer monolithic material is used for glycopeptides enrichment coupled with MALDI-TOF MS. By taking advantage of cobalt phthalocyanine including hydrogen b...

journal_title：Proteomics

authors： Zhang W,Song N,Zheng H,Feng W,Jia Q

更新日期：2018-10-01 00:00:00

abstract：:A method for the determination of the stoichiometry of protein complexes has been developed, which is based on proteolytic digestion of the complex, labeling with a fluorescent reagent, specific for amino or sulfhydryl groups, and separation by liquid chromatography with fluorescence and mass spectrometric detection. ...

journal_title：Proteomics

authors： Hochleitner EO,Sondermann P,Lottspeich F

更新日期：2004-03-01 00:00:00

abstract：:Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...

journal_title：Proteomics

authors： Agrawal GK,Rakwal R,Yonekura M,Kubo A,Saji H

更新日期：2002-08-01 00:00:00

abstract：:Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...

journal_title：Proteomics

authors： Lee AY,Goo Park S,Kho CW,Young Park S,Cho S,Lee SC,Lee DH,Myung PK,Park BC

更新日期：2004-11-01 00:00:00

abstract：:A simple and rapid method for characterizing hydrophobic integral membrane proteins and its utility for membrane proteomics using microcapillary liquid chromatography coupled on-line with tandem mass spectrometry (microLC-MS/MS) is described. The present technique does not rely on the use of detergents, strong organic...

journal_title：Proteomics

authors： Blonder J,Conrads TP,Yu LR,Terunuma A,Janini GM,Issaq HJ,Vogel JC,Veenstra TD

更新日期：2004-01-01 00:00:00

abstract：:Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. I...

journal_title：Proteomics

authors： Gilbert I,Schiffmann S,Rubenwolf S,Jensen K,Mai T,Albrecht C,Lankenau A,Beste G,Blank K,Gaub HE,Clausen-Schaumann H

更新日期：2004-05-01 00:00:00

abstract：:Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. sc...

journal_title：Proteomics

authors： Barnabas L,Ashwin NM,Kaverinathan K,Trentin AR,Pivato M,Sundar AR,Malathi P,Viswanathan R,Rosana OB,Neethukrishna K,Carletti P,Arrigoni G,Masi A,Agrawal GK,Rakwal R

更新日期：2016-04-01 00:00:00