Abstract:
:An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to FAB. Using MS/MS, the technique provides a fast, highly sensitive and reliable determination of the primary structure of unknown peptides. This procedure labels N-terminal fragments formed during MS/MS analysis, resulting in a simplification and faster interpretation of the spectra. The performance of the method has been tested with several synthetic peptides and applied to an efficient sequencing of the peptide map, using a nano-scale LC coupled on-line to a tandem mass spectrometer.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Noga MJ,Lewandowski JJ,Suder P,Silberring Jdoi
10.1002/pmic.200401319subject
Has Abstractpub_date
2005-11-01 00:00:00pages
4367-75issue
17eissn
1615-9853issn
1615-9861journal_volume
5pub_type
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