Proteomic analysis of the phytopathogenic fungus Botrytis cinerea during cellulose degradation.

abstract：:Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after i...

journal_title：Proteomics

authors： Iwata K,Café-Mendes CC,Schmitt A,Steiner J,Manabe T,Matsuzaki H,Falkai P,Turck CW,Martins-de-Souza D

更新日期：2013-12-01 00:00:00

abstract：:Prostate cancer is one of the leading cancers in men. Taking dietary supplements, such as fish oil (FO), which is rich in n-3 polyunsaturated fatty acids (PUFAs), has been employed as a strategy to lower prostate cancer risk and control disease progression. In this study, we investigated the global phosphoproteomic ch...

journal_title：Proteomics

authors： Zhao H,Pflug BR,Lai X,Wang M

更新日期：2016-09-01 00:00:00

abstract：:Selective activation of T helper subsets 1 (Th1) and 2 (Th2) plays a crucial role in different pathological conditions. Th1 cell response is involved in pathogenesis of autoimmune diseases, such as type II diabetes and multiple sclerosis, and Th2 cell response in pathogenesis of allergy and asthma. Cytokine interleuki...

journal_title：Proteomics

authors： Rosengren AT,Nyman TA,Lahesmaa R

更新日期：2005-08-01 00:00:00

abstract：:In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...

journal_title：Proteomics

authors： Grimplet J,Wheatley MD,Jouira HB,Deluc LG,Cramer GR,Cushman JC

更新日期：2009-05-01 00:00:00

abstract：:Surface-enhanced laser desorption/ionization (SELDI) time of flight (TOF) is a mass spectrometry technology for measuring the composition of a sampled protein mixture. A mass spectrum contains peaks corresponding to proteins in the sample. The peak areas are proportional to the measured concentrations of the correspon...

journal_title：Proteomics

authors： Dijkstra M,Roelofsen H,Vonk RJ,Jansen RC

更新日期：2006-10-01 00:00:00

abstract：:Rice is susceptible to cold stress and with a future of climatic instability we will be unable to produce enough rice to satisfy increasing demand. A thorough understanding of the molecular responses to thermal stress is imperative for engineering cultivars, which have greater resistance to low temperature stress. In ...

journal_title：Proteomics

authors： Neilson KA,Mariani M,Haynes PA

更新日期：2011-05-01 00:00:00

abstract：:Off-line coupling of capillary IEF (CIEF) with matrix-assisted laser desorption/ionization mass spectrometry was utilized for the analysis of human blood serum. Serum proteins were initially separated by CIEF, and fractions of the isoelectric separation were eluted sequentially to a MALDI-TOF MS sample target. During ...

journal_title：Proteomics

authors： Crowley TA,Hayes MA

更新日期：2005-09-01 00:00:00

abstract：:PROTICdb is a web-based application, mainly designed to store and analyze plant proteome data obtained by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and mass spectrometry (MS). The purposes of PROTICdb are (i) to store, track, and query information related to proteomic experiments, i.e., from tissue...

journal_title：Proteomics

authors： Ferry-Dumazet H,Houel G,Montalent P,Moreau L,Langella O,Negroni L,Vincent D,Lalanne C,de Daruvar A,Plomion C,Zivy M,Joets J

更新日期：2005-05-01 00:00:00

abstract：:An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-conce...

journal_title：Proteomics

authors： Zhang X,Shi L,Shu S,Wang Y,Zhao K,Xu N,Liu S,Roepstorff P

更新日期：2007-07-01 00:00:00

abstract：:The present review deals with the use of combinatorial ligand libraries, composed by hexapeptides, in the capture and concentration of the low-abundance proteome. This method, first reported in 2005, is compared with other current methodologies aimed at exploring the "deep proteome", such as: depletion of high-abundan...

journal_title：Proteomics

authors： Boschetti E,Righetti PG

更新日期：2009-03-01 00:00:00

abstract：:The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...

journal_title：Proteomics

authors： Ihling C,Sinz A

更新日期：2005-05-01 00:00:00

abstract：:Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...

journal_title：Proteomics

authors： Agrawal GK,Rakwal R,Yonekura M,Kubo A,Saji H

更新日期：2002-08-01 00:00:00

abstract：:Scanning MS by MALDI MS imaging (MALDI-MSI) creates large volumetric global datasets that describe the location and identity of ions registered at each sampling location. While thousands of ion peaks are recorded in a typical whole-tissue analysis, only a fraction of these measured molecules are purposefully scrutiniz...

journal_title：Proteomics

authors： Fehniger TE,Suits F,Végvári Á,Horvatovich P,Foster M,Marko-Varga G

更新日期：2014-04-01 00:00:00

abstract：:Tyrosol (TYR, 2-(4-hydroxyphenyl)ethanol), one of the main phenols in olive oil and olive fruit, significantly strengthens resistance to thermal and oxidative stress in the nematode Caenorhabditis elegans and extends its lifespan. To elucidate the cellular functions regulated by TYR, we have used a proteomic procedure...

journal_title：Proteomics

authors： Cañuelo A,Peragón J

更新日期：2013-10-01 00:00:00

abstract：:The G-protein complex is a cytoplasmic on-off molecular switch that is set by plasma membrane receptors that activate upon binding of its cognate extracellular agonist. In animals, the default setting is the "off" resting state, while in plants, the default state is constitutively "on" but repressed by a plasma membra...

journal_title：Proteomics

authors： Song G,Brachova L,Nikolau BJ,Jones AM,Walley JW

更新日期：2018-12-01 00:00:00

abstract：:To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by microLC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene ex...

journal_title：Proteomics

authors： Bernardini G,Arena S,Braconi D,Scaloni A,Santucci A

更新日期：2007-09-01 00:00:00

abstract：:Proteomics assays hold great promise for unraveling molecular events that underlie human diseases. Effective analysis of clinical samples is essential, but this task is considerably complicated by tissue heterogeneity. Laser capture microdissection (LCM) can be used to selectively isolate target cells from their nativ...

journal_title：Proteomics

authors： Umar A,Luider TM,Foekens JA,Pasa-Tolić L

更新日期：2007-01-01 00:00:00

abstract：:Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favored over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sampl...

journal_title：Proteomics

authors： Tubaon RM,Haddad PR,Quirino JP

更新日期：2017-10-01 00:00:00

abstract：:Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...

journal_title：Proteomics

authors： Ofir K,Berdichevsky Y,Benhar I,Azriel-Rosenfeld R,Lamed R,Barak Y,Bayer EA,Morag E

更新日期：2005-05-01 00:00:00

abstract：:Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...

journal_title：Proteomics

authors： Finehout EJ,Cantor JR,Lee KH

更新日期：2005-06-01 00:00:00

abstract：:Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated software, named IQuant, which integrates a postprocessing tool of protein identification and advanced statistical algorithms to process the MS/MS signals generated f...

journal_title：Proteomics

authors： Wen B,Zhou R,Feng Q,Wang Q,Wang J,Liu S

更新日期：2014-10-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:Many proteomic studies focus on quantitative aspects, using different stable isotope labeling techniques that require specialized software to analyze the generated data. Here we present jTraqX, an easy-to-use tool for processing and visualizing protein quantification data. jTraqX is platform independent and is compati...

journal_title：Proteomics

authors： Muth T,Keller D,Puetz SM,Martens L,Sickmann A,Boehm AM

更新日期：2010-03-01 00:00:00

abstract：:In this report we introduce a new concept "proteomic trajectory mapping" for the investigation of a complex phenomenon underlying biological transformation and transition. We define proteomic trajectory to be the kinetic trace of protein expression and present a successful proteomic trajectory mapping of complex molec...

journal_title：Proteomics

authors： Haniu H,Komori N,Takemori N,Singh A,Ash JD,Matsumoto H

更新日期：2006-06-01 00:00:00

abstract：:Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of im...

journal_title：Proteomics

authors： Luk VN,Fiddes LK,Luk VM,Kumacheva E,Wheeler AR

更新日期：2012-05-01 00:00:00

abstract：:Biotherapeutics, molecules produced from biological systems, require rigorous purification steps to remove impurities including host cell proteins (HCPs). Regulatory guidelines require manufacturers to monitor process-related impurities along the purification workflow. Mass spectrometry (MS) has recently been consider...

journal_title：Proteomics

authors： Trauchessec M,Hesse AM,Kraut A,Berard Y,Herment L,Fortin T,Bruley C,Ferro M,Manin C

更新日期：2021-01-18 00:00:00

abstract：:Familial hemiplegic migraine type 1 (FHM1) is caused by missense mutations in the CACNA1A gene that encodes the alpha1A pore-forming subunit of Ca(V)2.1 Ca(2+) channels. Knock-in (KI) transgenic mice expressing Ca(V)2.1 Ca(2+) channels with a human pathogenic FHM1 mutation reveal enhanced glutamatergic neurotransmissi...

journal_title：Proteomics

authors： Klychnikov OI,Li KW,Sidorov IA,Loos M,Spijker S,Broos LA,Frants RR,Ferrari MD,Mayboroda OA,Deelder AM,Smit AB,van den Maagdenberg AM

更新日期：2010-07-01 00:00:00

abstract：:To gain a better understanding of salt stress responses in plants, we used a proteomic approach to investigate changes in rice (Oryza sativa) root plasma-membrane-associated proteins following treatment with 150 mmol/L NaCl. With or without a 48 h salt treatment, plasma membrane fractions from root tip cells of a salt...

journal_title：Proteomics

authors： Cheng Y,Qi Y,Zhu Q,Chen X,Wang N,Zhao X,Chen H,Cui X,Xu L,Zhang W

更新日期：2009-06-01 00:00:00

abstract：:Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (...

journal_title：Proteomics

authors： Huang QY,Huang HQ

更新日期：2011-09-01 00:00:00

abstract：:Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...

journal_title：Proteomics

authors： Tan KT,Ang SJ,Tsai SY

更新日期：2020-03-01 00:00:00