Abstract:
:Familial hemiplegic migraine type 1 (FHM1) is caused by missense mutations in the CACNA1A gene that encodes the alpha1A pore-forming subunit of Ca(V)2.1 Ca(2+) channels. Knock-in (KI) transgenic mice expressing Ca(V)2.1 Ca(2+) channels with a human pathogenic FHM1 mutation reveal enhanced glutamatergic neurotransmission in the cortex. In this study, we employed an iTRAQ-based LC-LC MS/MS approach to identify differentially expressed proteins in cortical synapse proteomes of Cacna1a R192Q KI and wild-type mice. All expression differences determined were subtle and in the range of 10-30%. Observed upregulated proteins in the mutant mice are involved in processes, such as neurite outgrowth and actin dynamics, vesicle turnover, and glutamate transporters. Our data support the view that in Cacna1a R192Q KI mice, several compensatory mechanisms counterbalancing a dysregulated glutamatergic signaling have come into effect. We propose that such adaptation mechanisms at the synapse level may play a role in the pathophysiology of FHM and possibly in the common forms of migraine.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Klychnikov OI,Li KW,Sidorov IA,Loos M,Spijker S,Broos LA,Frants RR,Ferrari MD,Mayboroda OA,Deelder AM,Smit AB,van den Maagdenberg AMdoi
10.1002/pmic.200900733subject
Has Abstractpub_date
2010-07-01 00:00:00pages
2531-5issue
13eissn
1615-9853issn
1615-9861journal_volume
10pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Various types of unwanted and uncontrollable signal variations in MS-based metabolomics and proteomics datasets severely disturb the accuracies of metabolite and protein profiling. Therefore, pooled quality control (QC) samples are often employed in quality management processes, which are indispensable to the success ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900264
更新日期:2019-10-01 00:00:00
abstract::A large repertoire of immunological methods permits monitoring the interaction of antibodies with their specific antigen. However, recognition of a protein by a conformation-specific antibody represents a challenge because native conditions must be kept throughout the assay. Native immunoblotting of blue native gels c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900468
更新日期:2010-01-01 00:00:00
abstract::Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800166
更新日期:2019-09-01 00:00:00
abstract::The function of a protein is modulated by its abundance and its degree of specific post-translational modifications such as phosphorylation, glycosylation or truncation. Consequently, changes of protein concentration and the extent of their post-translational modifications has a great influence on the activity of intr...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200300581
更新日期:2003-11-01 00:00:00
abstract::More than 300 different types of protein post-translational modifications (PTMs) have been described, many of which are known to have pivotal roles in cellular physiology and disease. Nevertheless, only a handful of PTMs have been extensively investigated at the proteome level. Knowledge of protein substrates and thei...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200900398
更新日期:2009-10-01 00:00:00
abstract::Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800471
更新日期:2019-05-01 00:00:00
abstract::Autosomal dominant tubulointerstitial kidney disease associated to the MUC1 gene (ADTKD-MUC1; formerly MCKD1) belongs to a heterogeneous group of rare hereditary kidney diseases that is prototypically caused by frameshift mutations in the MUC1 repeat domain. The mutant MUC1 (insC) lacks the transmembrane domaine, exhi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700456
更新日期:2018-04-01 00:00:00
abstract::Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree select...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100183
更新日期:2012-04-01 00:00:00
abstract::In this report we introduce a new concept "proteomic trajectory mapping" for the investigation of a complex phenomenon underlying biological transformation and transition. We define proteomic trajectory to be the kinetic trace of protein expression and present a successful proteomic trajectory mapping of complex molec...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500813
更新日期:2006-06-01 00:00:00
abstract::We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500511
更新日期:2006-03-01 00:00:00
abstract::Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900502
更新日期:2010-02-01 00:00:00
abstract::Exosomes are deliverers of critically functional proteins, capable of transforming target cells in numerous cancers, including hepatocellular carcinoma (HCC). We hypothesize that the motility of HCC cells can be featured by comparative proteome of exosomes. Hence, we performed the super-SILAC-based MS analysis on the ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700103
更新日期:2017-07-01 00:00:00
abstract::The main goal of many proteomics experiments is an accurate and rapid quantification and identification of regulated proteins in complex biological samples. The bottleneck in quantitative proteomics remains the availability of efficient software to evaluate and quantify the tremendous amount of mass spectral data acqu...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900174
更新日期:2010-01-01 00:00:00
abstract::In a previous report we demonstrated protein kinase C (PKC)-mediated phosphorylation of the ligand-binding domain (LBD) of orphan nuclear receptor TR2. In this report, we provide the evidence of PKC-mediated phosphorylation of the DNA-binding domain (DBD) of TR2. Two PKC target sites were predicted within the DBD, at ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500068
更新日期:2006-01-01 00:00:00
abstract::The flavonoid flavone contained in a variety of fruits and vegetables was identified as a very potent apoptosis inducer in human colonic cancer cells. In search of the molecular targets of flavone action in HT-29 cells we analyzed changes in mRNA and protein expression levels by proteomics and oligonucleotide array te...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300754
更新日期:2004-08-01 00:00:00
abstract::Thirteen proteins (identified with 2-D gels and MALDI-TOF MS) are significantly altered during staurosporine-induced apoptosis in SH-SY5Y cells. To gain further insight into the integrated cellular response to apoptosis, we have investigated whether a network can be generated of direct and indirect interactions betwee...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900112
更新日期:2010-03-01 00:00:00
abstract::Sirtuins are a family of NAD(+)-dependent protein deacetylases that regulate cellular functions through deacetylation of a wide range of protein targets. Overexpression of Sir2, the first gene discovered in this family, is able to extend the life span in various organisms. The anti-aging effects of human homologues of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800738
更新日期:2009-05-01 00:00:00
abstract::Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000212
更新日期:2021-01-24 00:00:00
abstract::Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000548
更新日期:2011-03-01 00:00:00
abstract::Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500067
更新日期:2015-09-01 00:00:00
abstract::CRISPR-Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non-histone chromosomal protein HMG-14 (HMGN1) in regulating chromatin structure and tumor immunity, gene knockout of HMGN1 is performed by CRISPR in cancer cells and the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800438
更新日期:2019-07-01 00:00:00
abstract::Protein expression changes induced in thioglycolate-elicited peritoneal murine macrophages (M Phi) by infection with type III Group B Streptococcus (GBS) are described. Proteins from control M Phi and M Phi incubated 2 h with live or heat-inactivated GBS were separated by 2-DE. Proteins whose expression was significan...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900642
更新日期:2010-06-01 00:00:00
abstract::The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300519
更新日期:2014-11-01 00:00:00
abstract::The Ser/Thr/Tyr phosphoproteome of Bacillus subtilis was analyzed by a 2-D gel-based approach combining Pro-Q Diamond staining and [(33)P]-labeling. In exponentially growing B. subtilis cells 27 proteins could be identified after staining with Pro-Q Diamond and/or [(33)P]-labeling and one additional protein was labele...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700232
更新日期:2007-10-01 00:00:00
abstract::An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to F...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401319
更新日期:2005-11-01 00:00:00
abstract::Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000745
更新日期:2011-09-01 00:00:00
abstract::Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401078
更新日期:2005-05-01 00:00:00
abstract::Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700461
更新日期:2018-05-01 00:00:00
abstract::In the present study, changes in protein synthesis patterns after salt shock visualized by 35S-methionine labeling and the changed protein composition in salt-acclimated cells of the cyanobacterium Synechocystis sp. strain PCC 6803 were analyzed by a combination of 2-DE for protein separation and PMF for protein ident...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500538
更新日期:2006-05-01 00:00:00
abstract::A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500721
更新日期:2006-05-01 00:00:00