Quantitative proteomic analysis of cold-responsive proteins in rice.

abstract：:We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...

journal_title：Proteomics

authors： Ramsubramaniam N,Harris SD,Marten MR

更新日期：2014-11-01 00:00:00

abstract：:Plant secretomics is a newly emerging area of the plant proteomics field. It basically describes the global study of secreted proteins into the extracellular space of plant cell or tissue at any given time and under certain conditions through various secretory mechanisms. A combination of biochemical, proteomics and b...

journal_title：Proteomics

authors： Agrawal GK,Jwa NS,Lebrun MH,Job D,Rakwal R

更新日期：2010-02-01 00:00:00

abstract：:In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...

journal_title：Proteomics

authors： Hernandez P,Gras R,Frey J,Appel RD

更新日期：2003-06-01 00:00:00

abstract：:With recent progress in the analysis of the salivary proteome, the number of salivary proteins identified has increased dramatically. However, the physiological functions of many of the newly discovered proteins remain unclear. Closely related to the study of a protein's function is the identification of its interacti...

journal_title：Proteomics

authors： Siqueira WL,Lee YH,Xiao Y,Held K,Wong W

更新日期：2012-11-01 00:00:00

abstract：:Cancer research is striving toward new frontiers of assigning the correct personalized drug(s) to a given patient. However, extensive tumor heterogeneity poses a major obstacle. Tumors of the same type often respond differently to therapy, due to patient-specific molecular aberrations and/or untargeted tumor subpopula...

journal_title：Proteomics

authors： Kravchenko-Balasha N

更新日期：2020-07-01 00:00:00

abstract：:CRISPR-Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non-histone chromosomal protein HMG-14 (HMGN1) in regulating chromatin structure and tumor immunity, gene knockout of HMGN1 is performed by CRISPR in cancer cells and the...

journal_title：Proteomics

authors： Mehnert M,Li W,Wu C,Salovska B,Liu Y

更新日期：2019-07-01 00:00:00

abstract：:Identification of mucin-type O-glycosylated proteins with known functions in model organisms like Drosophila could provide keys to elucidate functions of the O-glycan moiety and proteomic analyses of O-glycoproteins in higher eukaryotes remain a challenge due to structural heterogeneity and a lack of efficient tools f...

journal_title：Proteomics

authors： Schwientek T,Mandel U,Roth U,Müller S,Hanisch FG

更新日期：2007-09-01 00:00:00

abstract：:Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...

journal_title：Proteomics

authors： Gao Y,Lee H,Kwon OK,Cheng Z,Tan M,Kim KT,Lee S

更新日期：2019-05-01 00:00:00

abstract：:Many of the emerging technologies for the global evaluation of gene expression, at both the RNA and protein level, are being applied to the problem of finding biomarkers for human disease progression. These analyses can be made difficult, however, by variation between samples that arises from both technical and nondis...

journal_title：Proteomics

authors： Steel LF,Shumpert D,Trotter M,Seeholzer SH,Evans AA,London WT,Dwek R,Block TM

更新日期：2003-05-01 00:00:00

abstract：:Current treatments for advanced prostate cancer focus on inhibition of the androgen receptor (AR) by androgen deprivation therapy (ADT). However, complex interactions mediated by tumor suppressors, oncogenes, aberrations of AR expression, or de novo androgen production have been shown to induce the adaptive response o...

journal_title：Proteomics

authors： Soekmadji C,Rockstroh A,Ramm GA,Nelson CC,Russell PJ

更新日期：2017-12-01 00:00:00

abstract：:Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...

journal_title：Proteomics

authors： Kunec D,Nanduri B,Burgess SC

更新日期：2009-05-01 00:00:00

abstract：:More than 300 different types of protein post-translational modifications (PTMs) have been described, many of which are known to have pivotal roles in cellular physiology and disease. Nevertheless, only a handful of PTMs have been extensively investigated at the proteome level. Knowledge of protein substrates and thei...

journal_title：Proteomics

authors： Zhao Y,Jensen ON

更新日期：2009-10-01 00:00:00

abstract：:The glycan structures of the major and more than ten minor populated isoforms of antithrombin (AT) were determined after separation of the isoforms by IEF using IPG strips. The bands excised from the gel were reduced, derivatized by iodoacetamide and submitted to tryptic digestion. The digest was analyzed by RP-HPLC-E...

journal_title：Proteomics

authors： Plematl A,Demelbauer UM,Josic D,Rizzi A

更新日期：2005-10-01 00:00:00

abstract：:One of the major challenges facing protein analysis is the dynamic range of protein expression within massively complex samples (Corthals, G. L. et al.., Electrophoresis 2000, 21, 1104-1115). In plasma this difference is as great as ten orders of magnitude, and this is currently beyond the range of detection achievabl...

journal_title：Proteomics

authors： Wasinger VC,Locke VL,Raftery MJ,Larance M,Rothemund D,Liew A,Bate I,Guilhaus M

更新日期：2005-08-01 00:00:00

abstract：:To better understand the underlying molecular basis of leaf development in maize, a reference map of nuclear proteins in basal region of seedling leaf was established using a combination of 2DE and MALDI-TOF-MS. In total, 441 reproducible protein spots in nuclear proteome of maize leaf basal region were detected with ...

journal_title：Proteomics

authors： Guo B,Chen Y,Li C,Wang T,Wang R,Wang B,Hu S,Du X,Xing H,Song X,Yao Y,Sun Q,Ni Z

更新日期：2014-05-01 00:00:00

abstract：:Accumulation of proteins in inclusions in neurological disorders is partly due to dysfunction of the ubiquitin-proteasome system. Proteasomal dysfunction may be caused by misexpression of one or more of its subunits. A large number of antibodies reactive with proteasome subunits were screened on material from patients...

journal_title：Proteomics

authors： Zouambia M,Fischer DF,Hobo B,De Vos RA,Hol EM,Varndell IM,Sheppard PW,Van Leeuwen FW

更新日期：2008-03-01 00:00:00

abstract：:Leishmaniasis is a zoonotic disease caused by the species of the genus Leishmania, flagellated protozoa that multiply inside mammalian macrophages and are transmitted by the bite of the sandfly. The disease is widespread and due to the lack of fully effective treatment and vaccination the search for new drugs and immu...

journal_title：Proteomics

authors： Dea-Ayuela MA,Rama-Iñiguez S,Bolás-Fernández F

更新日期：2006-07-01 00:00:00

abstract：:Niemann-Pick disease, type C1 (NPC1) is a fatal, autosomal recessive, neurodegenerative disorder caused by mutations in the NPC1 gene. As a result of the genetic defect, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal/lysosomal system causing both visceral and neurological def...

journal_title：Proteomics

authors： Pergande MR,Serna-Perez F,Mohsin SB,Hanek J,Cologna SM

更新日期：2019-09-01 00:00:00

abstract：:Following transmission to the vertebrate host, the protozoan parasite Leishmania donovani differentiates into the pathogenic amastigote stage that is adapted for intracellular survival. This developmental transition is induced by environmental factors including elevated temperature and acidic pH and is likely transduc...

journal_title：Proteomics

authors： Morales MA,Watanabe R,Laurent C,Lenormand P,Rousselle JC,Namane A,Späth GF

更新日期：2008-01-01 00:00:00

abstract：:Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...

journal_title：Proteomics

authors： Davoodi J,Kelly J,Gendron NH,MacKenzie AE

更新日期：2007-06-01 00:00:00

abstract：:Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...

journal_title：Proteomics

authors： Kamita M,Mori T,Sakai Y,Ito S,Gomi M,Miyamoto Y,Harada A,Niida S,Yamada T,Watanabe K,Ono M

更新日期：2015-05-01 00:00:00

abstract：:Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A2/B1, and A3) play an important role in proliferation, although the functional overlap among members of hnRNPs A/B remains largely unknown. In this study, we have employed RNAi knockdown and proteomic approaches to investigate the biological functions of h...

journal_title：Proteomics

authors： Yeh YM,Chen CY,Huang PR,Hsu CW,Wu CC,Wang TC

更新日期：2014-06-01 00:00:00

abstract：:Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...

journal_title：Proteomics

authors： Tsai JJ,Kuo HC,Lee KF,Tsai TH

更新日期：2015-11-01 00:00:00

abstract：:With an emphasis on obtaining a multitude of high quality tandem mass spectrometry spectra for protein identification, instrumental parameters are described for the liquid chromatography-tandem mass spectrometry analysis of trypsin digested unfractionated urine using a hybrid quadrupole-time-of-flight (Q-TOF) mass spe...

journal_title：Proteomics

authors： Davis MT,Spahr CS,McGinley MD,Robinson JH,Bures EJ,Beierle J,Mort J,Yu W,Luethy R,Patterson SD

更新日期：2001-01-01 00:00:00

abstract：:In this work, for the first time, perfluorinated magnetic mesoporous microspheres were designed and synthesized for the highly specific enrichment of fluorous-derivatized phosphopeptides through the unique fluorine-fluorine interactions. The perfluorinated magnetic mesoporous microspheres were prepared through a surfa...

journal_title：Proteomics

authors： Zhao M,Deng C

更新日期：2016-04-01 00:00:00

abstract：:Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an ...

journal_title：Proteomics

authors： Schueder F,Unterauer EM,Ganji M,Jungmann R

更新日期：2020-10-08 00:00:00

abstract：:The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...

journal_title：Proteomics

authors： Ritz D,Gloger A,Weide B,Garbe C,Neri D,Fugmann T

更新日期：2016-05-01 00:00:00

abstract：:Entamoeba histolytica is known for its extraordinary capacity to destroy human tissues, leading to invasive diseases such as ulcerative colitis or extra-intestinal abscesses. In order to identify the virulence factors of this parasite phenotypes and proteomes of two recently identified genetically related cell lines (...

journal_title：Proteomics

authors： Biller L,Schmidt H,Krause E,Gelhaus C,Matthiesen J,Handal G,Lotter H,Janssen O,Tannich E,Bruchhaus I

更新日期：2009-09-01 00:00:00

abstract：:The accurate and precise absolute abundance of proteins can be determined using mass spectrometry by spiking the sample with stable isotope-labeled standards. In this study, we developed a strategy of hierarchical use of peptide-concatenated standards (PCSs) to quantify more proteins over a wider dynamic range. Multip...

journal_title：Proteomics

authors： Kito K,Okada M,Ishibashi Y,Okada S,Ito T

更新日期：2016-05-01 00:00:00

abstract：:The HUPO Brain Proteome Project (HUPO BPP) held its 12th workshop in Toronto on 26 September 2009 prior to the HUPO VIII World Congress. The principal aim of this project is to obtain a better understanding of neurodiseases and ageing, with the ultimate objective of discovering prognostic and diagnostic biomarkers, in...

journal_title：Proteomics

authors： Gröttrup B,Eisenacher M,Stephan C,Marcus K,Lee B,Meyer HE,Park YM

更新日期：2010-06-01 00:00:00