Abstract:
:More than 300 different types of protein post-translational modifications (PTMs) have been described, many of which are known to have pivotal roles in cellular physiology and disease. Nevertheless, only a handful of PTMs have been extensively investigated at the proteome level. Knowledge of protein substrates and their PTM sites is key to dissection of PTM-mediated cellular processes. The past several years have seen a tremendous progress in developing MS-based proteomics technologies for global PTM analysis, including numerous studies of yeast and other microbes. Modification-specific enrichment techniques combined with advanced MS/MS methods and computational data analysis have revealed a surprisingly large extent of PTMs in proteins, including multi-site, cooperative modifications in individual proteins. We review some of the current strategies employed for enrichment and detection of PTMs in modification-specific proteomics.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Zhao Y,Jensen ONdoi
10.1002/pmic.200900398subject
Has Abstractpub_date
2009-10-01 00:00:00pages
4632-41issue
20eissn
1615-9853issn
1615-9861journal_volume
9pub_type
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