Abstract:
:Recently, several studies employed various proteomic approaches to define diabetes-induced changes in renal proteins. However, functional significance of those datasets in diabetic nephropathy remained unclear. We thus performed integrative proteome network analysis of such datasets followed by various targeted functional studies in distal renal tubular cells treated with high glucose (HG) (25 mM) compared to normal glucose (NG) (5.5 mM) and NG + mannitol (M) (5.5 + 19.5 mM). The data showed that at 96 h when cell proliferation/death, tight junction protein and β-/F-actin expression and organization, and transepithelial resistance remained unchanged, only HG caused increased levels of HSP90, HSP70, and HSP60, and increased accumulation of intracellular protein aggregates. In addition, HG also induced overproduction of intracellular ROS, decreased catalase level, increased level of oxidatively modified proteins, increased intracellular ATP level, and defective transepithelial Ca2+ transport. However, both HG and M increased the levels of ubiquitinated proteins. Taken together, this study demonstrated significant perturbations of distal renal tubular cells induced by HG based on targeted functional studies guided by integrative proteome network analysis. These data may, at least in part, lead to better understanding of the pathogenic mechanisms of diabetic nephropathy.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Aluksanasuwan S,Khamchun S,Thongboonkerd Vdoi
10.1002/pmic.201700151subject
Has Abstractpub_date
2017-09-01 00:00:00issue
17-18eissn
1615-9853issn
1615-9861journal_volume
17pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100186
更新日期:2011-10-01 00:00:00
abstract::A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differenti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900695
更新日期:2010-04-01 00:00:00
abstract::The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201700223
更新日期:2019-05-01 00:00:00
abstract::In this work, for the first time, perfluorinated magnetic mesoporous microspheres were designed and synthesized for the highly specific enrichment of fluorous-derivatized phosphopeptides through the unique fluorine-fluorine interactions. The perfluorinated magnetic mesoporous microspheres were prepared through a surfa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500323
更新日期:2016-04-01 00:00:00
abstract::As we transition from genomics to the challenges of the functional proteome, new tools to explore the expression of proteins within tissue are essential. We have developed a method of transferring proteins from a formalin fixed, paraffin embedded tissues section to a stack of membranes which is then probed with antibo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401343
更新日期:2006-02-01 00:00:00
abstract::Mechanisms of milk lipid secretion are highly controversial. Analyzing the fine protein composition of the "milk fat globule membrane" (MFGM), the triple-layered membrane surrounding milk lipid droplets (LDs) can provide mechanistic clues to better understand LD biosynthesis and secretion pathways in mammary epithelia...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400245
更新日期:2015-07-01 00:00:00
abstract::Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated software, named IQuant, which integrates a postprocessing tool of protein identification and advanced statistical algorithms to process the MS/MS signals generated f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300361
更新日期:2014-10-01 00:00:00
abstract::The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertili...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800588
更新日期:2009-02-01 00:00:00
abstract::A monolithic enzymatic microreactor was prepared in a fused-silica capillary by in situ polymerization of acrylamide, glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, followed by ammonia solution treatment, glutaraldehyde activa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500234
更新日期:2006-01-01 00:00:00
abstract::The pilot phase of the Human Brain Proteome Project as a part of the Human Proteome Organisation has just been started. In two pilot studies, 18 different laboratories are analyzing mouse brains of three age stages and human brain autopsy versus biopsy material, respectively. The overall aim is to elucidate the portfo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400867
更新日期:2004-08-01 00:00:00
abstract::Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300031
更新日期:2013-11-01 00:00:00
abstract::The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500001
更新日期:2005-11-01 00:00:00
abstract::The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic dig...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000039
更新日期:2010-07-01 00:00:00
abstract::O-GlcNAcylation is a dynamic PTM of nuclear and cytoplasmic proteins, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase, which catalyze the addition and removal of O-GlcNAc, respectively. This modification is associated with glucose metabolism, which plays important roles in many diseases including cancer. Altho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200126
更新日期:2013-07-01 00:00:00
abstract::Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700760
更新日期:2008-06-01 00:00:00
abstract::This paper proposes a novel profiling method for SELDI-TOF and MALDI-TOF MS data that integrates a novel peak detection method based on modified smoothed non-linear energy operator, correlation-based peak selection and Bayesian additive regression trees. The peak detection and classification performance of the propose...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800502
更新日期:2009-09-01 00:00:00
abstract::New insights on cellular heterogeneity in the last decade provoke the development of a variety of single cell omics tools at a lightning pace. The resultant high-dimensional single cell data generated by these tools require new theoretical approaches and analytical algorithms for effective visualization and interpreta...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201600267
更新日期:2017-02-01 00:00:00
abstract::Scanning MS by MALDI MS imaging (MALDI-MSI) creates large volumetric global datasets that describe the location and identity of ions registered at each sampling location. While thousands of ion peaks are recorded in a typical whole-tissue analysis, only a fraction of these measured molecules are purposefully scrutiniz...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300431
更新日期:2014-04-01 00:00:00
abstract::Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401268
更新日期:2005-06-01 00:00:00
abstract::Biology is increasingly dependent on large-scale analysis, such as proteomics, creating a requirement for efficient bioinformatics. Bioinformatic predictions of biological functions rely upon correctly annotated database sequences, and the presence of inaccurately annotated or otherwise poorly described sequences intr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600034
更新日期:2016-09-01 00:00:00
abstract::Cells are a fundamental unit of life, and the ability to study the phenotypes and behaviors of individual cells is crucial to understanding the workings of complex biological systems. Cell phenotypes (epigenomic, transcriptomic, proteomic, and metabolomic) exhibit dramatic heterogeneity between and within the differen...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201700312
更新日期:2018-09-01 00:00:00
abstract::Sequencing of the human genome opened the way to the exploration of the proteome and this has lead to the identification of large numbers of proteins in complex biological samples. The identification of diagnostic patterns in samples taken from patients to aid diagnosis is in the early stages of development. The solut...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700665
更新日期:2008-02-01 00:00:00
abstract::Adiponectin is a fat cell-secreted hormone with antidiabetic and anti-inflammatory activities. The reduced adiponectin levels are associated with obesity-related metabolic syndrome. Replenishment of this hormone into animal models can improve insulin sensitivity, decrease blood glucose and lipid levels, and prevent th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500840
更新日期:2006-07-01 00:00:00
abstract::The HUPO Brain Proteome Project (HUPO BPP) held its 12th workshop in Toronto on 26 September 2009 prior to the HUPO VIII World Congress. The principal aim of this project is to obtain a better understanding of neurodiseases and ageing, with the ultimate objective of discovering prognostic and diagnostic biomarkers, in...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.201090042
更新日期:2010-06-01 00:00:00
abstract::Voltage-dependent anion channel (VDAC) proteins are small, abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apopt...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300734
更新日期:2004-05-01 00:00:00
abstract::Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500129
更新日期:2006-03-01 00:00:00
abstract::Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300032
更新日期:2014-07-01 00:00:00
abstract::The thermophilic bacterium Bacillus stearothermophilus P1 is unique in its ability to thrive in extreme environments such as high temperatures or high pH conditions. The study of cold shock response is very interesting and interpreted as a shock response to express the genes involved in synthesis of specific proteins....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200209)2:9<1316::AID-PROT1316>3.
更新日期:2002-09-01 00:00:00
abstract::Normal sera contain a large number of naturally occurring autoantibodies which can mask important disease-associated ones. Western blotting has evolved as the most important tool to demonstrate autoantibodies in autoimmune diseases, because of its ability to simultaneous screening for a wide spectrum of different anti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300375
更新日期:2003-06-01 00:00:00
abstract::Liquid Chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS) is a powerful analytical technique for the identification and mass analysis of complex protein mixtures. Here, we present a combination of methods developed for the extensive/deep proteomic analysis of purified ribosome/mRNA particles assembled i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400628
更新日期:2015-07-01 00:00:00