Proteomic analyses of genes regulated by heterogeneous nuclear ribonucleoproteins A/B in Jurkat cells.

abstract：:Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...

journal_title：Proteomics

authors： Hu S,Xie Y,Ramachandran P,Ogorzalek Loo RR,Li Y,Loo JA,Wong DT

更新日期：2005-04-01 00:00:00

abstract：:Phosphorylation of proteins is one of the most prominent PTMs and for instance a key regulator of signal transduction. In order to improve our understanding of cellular phosphorylation events, considerable effort has been devoted to improving the analysis of phosphorylation by MS-based proteomics. Different enrichment...

journal_title：Proteomics

authors： Eyrich B,Sickmann A,Zahedi RP

更新日期：2011-02-01 00:00:00

abstract：:A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera g...

journal_title：Proteomics

authors： Fernández-Arenas E,Molero G,Nombela C,Diez-Orejas R,Gil C

更新日期：2004-04-01 00:00:00

abstract：:Since detergent-resistant lipid rafts play important roles in the signal transduction for myogenesis, their comprehensive proteomic analysis could provide new insights to understand their function in myotubes. Here, the detergent-resistant lipid rafts were isolated from C2C12 myotubes and analyzed by capillary RPLC/MS...

journal_title：Proteomics

authors： Kim BW,Lee JW,Choo HJ,Lee CS,Jung SY,Yi JS,Ham YM,Lee JH,Hong J,Kang MJ,Chi SG,Hyung SW,Lee SW,Kim HM,Cho BR,Min DS,Yoon G,Ko YG

更新日期：2010-07-01 00:00:00

abstract：:High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...

journal_title：Proteomics

authors： Majoul T,Bancel E,Triboï E,Ben Hamida J,Branlard G

更新日期：2003-02-01 00:00:00

abstract：:DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...

journal_title：Proteomics

authors： Timms JF,Cramer R

更新日期：2008-12-01 00:00:00

abstract：:Proteome analysis of human hepatocellular carcinoma tissues was conducted using two-dimensional difference gel electrophoresis coupled with mass spectrometry. Paired samples from the normal and tumor region of resected human liver were labeled with Cy3 and Cy5, respectively while the pooled standard sample was labeled...

journal_title：Proteomics

authors： Liang CR,Leow CK,Neo JC,Tan GS,Lo SL,Lim JW,Seow TK,Lai PB,Chung MC

更新日期：2005-05-01 00:00:00

abstract：:Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...

journal_title：Proteomics

authors： Chaker S,Kashat L,Voisin S,Kaur J,Kak I,MacMillan C,Ozcelik H,Siu KW,Ralhan R,Walfish PG

更新日期：2013-03-01 00:00:00

abstract：:Mitochondria were isolated from whole hearts of Dahl salt sensitive (SS) and chromosome 13 consomic control (SS.13BN/Mcwi) rats using a mechanical homogenization process followed by density centrifugation. The proteins present in the two mitochondria preparations were quantified; equal amounts of protein from each sam...

journal_title：Proteomics

authors： Smith JR,Matus IR,Beard DA,Greene AS

更新日期：2008-02-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:CREC proteins constitute a family of EF-hand calcium binding proteins localized to the secretory pathway. Calumenin is the only member known to be secreted. Recently, it was shown that thrombin-activated thrombocytes liberate calumenin, which also is found in atherosclerotic lesions but not in normal vasculature. To s...

journal_title：Proteomics

authors： Ostergaard M,Hansen GA,Vorum H,Honoré B

更新日期：2006-06-01 00:00:00

abstract：:Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favored over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sampl...

journal_title：Proteomics

authors： Tubaon RM,Haddad PR,Quirino JP

更新日期：2017-10-01 00:00:00

abstract：:MDM2 is a multidomain protein that functions as an E3 ubiquitin ligase, transcription repressor, mRNA-binding protein, translation factor, and molecular chaperone. The small molecule Nutlin-3 has been engineered to bind to the N-terminal hydrophobic pocket domain of MDM2. This binding of Nutlin-3 has two consequences:...

journal_title：Proteomics

authors： Hernychova L,Man P,Verma C,Nicholson J,Sharma CA,Ruckova E,Teo JY,Ball K,Vojtesek B,Hupp TR

更新日期：2013-08-01 00:00:00

abstract：:GlycoMod (http://www.expasy.ch/tools/glycomod/) is a software tool designed to find all possible compositions of a glycan structure from its experimentally determined mass. The program can be used to predict the composition of any glycoprotein-derived oligosaccharide comprised of either underivatised, methylated or ac...

journal_title：Proteomics

authors： Cooper CA,Gasteiger E,Packer NH

更新日期：2001-02-01 00:00:00

abstract：:Protein interactions have been at the focus of computational biology in recent years. In particular, interest has come from two different communities--structural and systems biology. Here, we will discuss key systems and structural biology methods that have been used for analysis and prediction of protein-protein inte...

journal_title：Proteomics

authors： Hooda Y,Kim PM

更新日期：2012-05-01 00:00:00

abstract：:We previously reported that NSC606985, a new camptothecin analog, induces apoptosis of acute myeloid leukemic cells, which is triggered by proteolytic activation of protein kinase C delta (PKC delta). Here, we performed quantitative proteomic analysis of NSC606985-treated and untreated leukemic U937 cells with two-dim...

journal_title：Proteomics

authors： Zheng Y,Wang LS,Xia L,Han YH,Liao SH,Wang XL,Cheng JK,Chen GQ

更新日期：2009-04-01 00:00:00

abstract：:Uni- or multidimensional microcapillary liquid chromatography (microLC) matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry (MS/MS) approaches have gained significant attention for quantifying and identifying proteins in complex biological samples. The off-line coupling of microLC with MS quan...

journal_title：Proteomics

authors： Graber A,Juhasz PS,Khainovski N,Parker KC,Patterson DH,Martin SA

更新日期：2004-02-01 00:00:00

abstract：:In proteomic research, two-dimensional electrophoresis (2-D) is an important tool for investigating differential patterns of qualitative and quantitative protein expression. The strength of the technique is due to its unrivalled power of being able to separate simultaneously thousands of proteins. The key to the compa...

journal_title：Proteomics

authors： Veeser S,Dunn MJ,Yang GZ

更新日期：2001-07-01 00:00:00

abstract：:Aluminum (Al) toxicity is a major constraint for plant root development and growth as well as crop yield in acidic soils, which constitute approximately 40% of the potentially arable lands worldwide. The mechanisms of Al tolerance in plants are not well understood. As a whole systems approach, proteomic techniques hav...

journal_title：Proteomics

authors： Zheng L,Lan P,Shen RF,Li WF

更新日期：2014-03-01 00:00:00

abstract：:The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...

journal_title：Proteomics

authors： Mehaffy C,Hess A,Prenni JE,Mathema B,Kreiswirth B,Dobos KM

更新日期：2010-05-01 00:00:00

abstract：:CRISPR-Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non-histone chromosomal protein HMG-14 (HMGN1) in regulating chromatin structure and tumor immunity, gene knockout of HMGN1 is performed by CRISPR in cancer cells and the...

journal_title：Proteomics

authors： Mehnert M,Li W,Wu C,Salovska B,Liu Y

更新日期：2019-07-01 00:00:00

abstract：:The recent development in immune checkpoint inhibitors and chimeric antigen receptor (CAR) T-cells in the treatment of cancer has not only demonstrated the potency of utilizing T-cell reactivity for cancer therapy, but has also highlighted the need for developing new approaches to discover targets suitable for such no...

journal_title：Proteomics

authors： Ternette N,Olde Nordkamp MJM,Müller J,Anderson AP,Nicastri A,Hill AVS,Kessler BM,Li D

更新日期：2018-06-01 00:00:00

abstract：:Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (...

journal_title：Proteomics

authors： Wang MC,Peng ZY,Li CL,Li F,Liu C,Xia GM

更新日期：2008-04-01 00:00:00

abstract：:Deciphering a complete landscape of protein-RNA interactions in the human proteome remains an elusive challenge. We computationally elucidate RNA binding proteins (RBPs) using an approach that complements previous efforts. We employ two modern complementary sequence-based methods that provide accurate predictions from...

journal_title：Proteomics

authors： Chowdhury S,Zhang J,Kurgan L

更新日期：2018-11-01 00:00:00

abstract：:The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...

journal_title：Proteomics

authors： Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

更新日期：2013-11-01 00:00:00

abstract：:Antibody microarrays have great potential for significant value in biological research. Cancer research in particular could benefit from the unique experimental capabilities of this technology. This article examines the current state of antibody microarray technological developments and assay formats, along with a rev...

journal_title：Proteomics

authors： Haab BB

更新日期：2003-11-01 00:00:00

abstract：:Calcineurin is a heterodimeric serine/threonine protein phosphatase, important for many cellular processes such as T-cell regulation, cardiac hypertrophy and kidney development. We previously reported the characterization of Caenorhabditis elegans calcineurin mutants as providing a simple but excellent genetic model s...

journal_title：Proteomics

authors： Ahn DH,Singaravelu G,Lee S,Ahnn J,Shim YH

更新日期：2006-02-01 00:00:00

abstract：:In the present study, changes in protein synthesis patterns after salt shock visualized by 35S-methionine labeling and the changed protein composition in salt-acclimated cells of the cyanobacterium Synechocystis sp. strain PCC 6803 were analyzed by a combination of 2-DE for protein separation and PMF for protein ident...

journal_title：Proteomics

authors： Fulda S,Mikkat S,Huang F,Huckauf J,Marin K,Norling B,Hagemann M

更新日期：2006-05-01 00:00:00

abstract：:Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins ...

journal_title：Proteomics

authors： Janjanam J,Jamwal M,Singh S,Kumar S,Panigrahi AK,Hariprasad G,Jena MK,Anand V,Kumar S,Kaushik JK,Dang AK,Mukesh M,Mishra BP,Srinivasan A,Reddy VS,Mohanty AK

更新日期：2013-11-01 00:00:00

abstract：:Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either (13) C-naphthalene or (13) C-fluorene ...

journal_title：Proteomics

authors： Herbst FA,Bahr A,Duarte M,Pieper DH,Richnow HH,von Bergen M,Seifert J,Bombach P

更新日期：2013-10-01 00:00:00