Large-scale identification of proteins in human salivary proteome by liquid chromatography/mass spectrometry and two-dimensional gel electrophoresis-mass spectrometry.

abstract：:We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...

journal_title：Proteomics

authors： Ramsubramaniam N,Harris SD,Marten MR

更新日期：2014-11-01 00:00:00

abstract：:Two Chinese bread wheat cultivars, Jinghua 9 and Zhongmai 175, distinct in grain weight and dough quality, were used to study proteome changes in the embryo and endosperm during grain development using a two-dimensional difference gel electrophoresis (2D-DIGE)-based proteomics approach. In total, 138 and 127 different...

journal_title：Proteomics

authors： Cao H,He M,Zhu C,Yuan L,Dong L,Bian Y,Zhang W,Yan Y

更新日期：2016-05-01 00:00:00

abstract：:Aspergillus fumigatus faces abrupt changes in oxygen concentrations at the site of infection. An increasing number of studies has demonstrated that elevated production of intracellular reactive oxygen species (ROS) under low oxygen conditions plays a regulatory role in modulating cellular responses for adaptation to h...

journal_title：Proteomics

authors： Shekhova E,Ivanova L,Krüger T,Stroe MC,Macheleidt J,Kniemeyer O,Brakhage AA

更新日期：2019-03-01 00:00:00

abstract：:Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...

journal_title：Proteomics

authors： Steevensz A,Gombar R,Vergilino R,Cristescu ME,Vacratsis PO

更新日期：2018-08-01 00:00:00

abstract：:Shewanella inhabit a wide variety of niches in nature and can utilize a broad spectrum of electron acceptors under anaerobic conditions. How they modulate their gene expression to adapt is poorly understood. ArcA, homologue of a global regulator controlling hundreds of genes involved in aerobic and anaerobic respirati...

journal_title：Proteomics

authors： Yuan J,Wei B,Lipton MS,Gao H

更新日期：2012-06-01 00:00:00

abstract：:This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused o...

journal_title：Proteomics

authors： Dumont D,Noben JP,Verhaert P,Stinissen P,Robben J

更新日期：2006-09-01 00:00:00

abstract：:Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...

journal_title：Proteomics

authors： Agrawal GK,Rakwal R,Yonekura M,Kubo A,Saji H

更新日期：2002-08-01 00:00:00

abstract：:Cyanobacteria are oxygenic photosynthetic prokaryotes and play a crucial role in the Earth's carbon and nitrogen cycles. The photoautotrophic cyanobacterium Anabaena sp. PCC 7120 has the ability to fix atmospheric nitrogen in heterocysts and produce hydrogen as a byproduct through a nitrogenase. In order to improve hy...

journal_title：Proteomics

authors： Kourpa K,Manarolaki E,Lyratzakis A,Strataki V,Rupprecht F,Langer JD,Tsiotis G

更新日期：2019-10-01 00:00:00

abstract：:In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes ...

journal_title：Proteomics

authors： Laria AE,Messineo S,Arcidiacono B,Varano M,Chiefari E,Semple RK,Rocha N,Russo D,Cuda G,Gaspari M,Brunetti A,Foti DP

更新日期：2018-04-01 00:00:00

abstract：:Gan et al. (Proteomics 2013, 13, 3117-3123) described a new "macropore" protocol for effective protein digestion by trypsin suitable for a wide range of pH including acidic pH. It was effective not only in experiments with solutions of a model protein (myoglobin), but also with a subfraction of rat liver cytosol. This...

journal_title：Proteomics

authors： Medvedev AE

更新日期：2013-11-01 00:00:00

abstract：:The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...

journal_title：Proteomics

authors： Kasyapa C,Gu TL,Nagarajan L,Polakiewicz R,Cowell JK

更新日期：2009-08-01 00:00:00

abstract：:The plenary session of the Proteomics Standards Initiative of the Human Proteome Organisation at the 6th annual HUPO world congress updated the delegates on the current status of the ongoing work of this group. The new MS interchange format, mzML, had been entered into the HUPO document process immediately prior to th...

journal_title：Proteomics

authors： Orchard S,Martens L,Tasman J,Binz PA,Albar JP,Hermjakob H

更新日期：2008-04-01 00:00:00

abstract：:In the post-genomic era, mass spectrometry is destined to fulfil a central role in biomedical research, and it is in the area of protein identification that mass spectrometry is now most rapidly expanding. An important identification method is to subject a protein to proteolysis and determine the resulting peptide mas...

journal_title：Proteomics

authors： Griffiths WJ,Jonsson AP

更新日期：2001-08-01 00:00:00

abstract：:Human plasma is the most clinically valuable specimen, containing not only a dynamic concentration range of protein components, but also several groups of high-abundance proteins that seriously interfere with the detection of low-abundance potential biomarker proteins. To establish a high-throughput method for efficie...

journal_title：Proteomics

authors： Cho SY,Lee EY,Lee JS,Kim HY,Park JM,Kwon MS,Park YK,Lee HJ,Kang MJ,Kim JY,Yoo JS,Park SJ,Cho JW,Kim HS,Paik YK

更新日期：2005-08-01 00:00:00

abstract：:The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...

journal_title：Proteomics

authors： Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

更新日期：2019-11-01 00:00:00

abstract：:The senescence-accelerated mouse (SAM) is a useful animal model to study aging or age-associated disorders due to its inherited aging phenotype. To investigate proteins involved in the aging process in liver, we compared the young (4- or 20-week old) and the aged group (50-week-old) of SAMP8 (short life span) and SAMR...

journal_title：Proteomics

authors： Cho YM,Bae SH,Choi BK,Cho SY,Song CW,Yoo JK,Paik YK

更新日期：2003-10-01 00:00:00

abstract：:Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/cGMP (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecul...

journal_title：Proteomics

authors： Zhang G,Wong YH,Zhang Y,He LS,Xu Y,Qian PY

更新日期：2015-11-01 00:00:00

abstract：:Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...

journal_title：Proteomics

authors： Ebhardt HA,Sabidó E,Hüttenhain R,Collins B,Aebersold R

更新日期：2012-04-01 00:00:00

abstract：:Antibody-based proteomics applied to tissue microarray (TMA) technology provides a very efficient means of visualizing and locating antigen expression in large collections of normal and pathological tissue samples. To characterize antigen expression on TMAs, the use of image analysis methods avoids the effects of huma...

journal_title：Proteomics

authors： Decaestecker C,Lopez XM,D'Haene N,Roland I,Guendouz S,Duponchelle C,Berton A,Debeir O,Salmon I

更新日期：2009-10-01 00:00:00

abstract：:Cofilin, an actin-binding protein, regulates the rate, nature and extent of assembly of the actin cytoskeleton. Native Phast gels show that the addition of cofilin to an actin-DNase I complex (74 kDa) results in the formation of a ternary complex of 94 kDa indicating an equimolar stoichiometry in the ternary complex. ...

journal_title：Proteomics

authors： Nosworthy NJ,Kekic M,dos Remedios CG

更新日期：2001-12-01 00:00:00

abstract：:Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...

journal_title：Proteomics

authors： Westers L,Westers H,Zanen G,Antelmann H,Hecker M,Noone D,Devine KM,van Dijl JM,Quax WJ

更新日期：2008-07-01 00:00:00

abstract：:Staphylococcus aureus is an opportunistic human pathogen, which can cause life-threatening disease. Proteome analyses of the bacterium can provide new insights into its pathophysiology and important facets of metabolic adaptation and, thus, aid the recognition of targets for intervention. However, the value of such pr...

journal_title：Proteomics

authors： Depke M,Michalik S,Rabe A,Surmann K,Brinkmann L,Jehmlich N,Bernhardt J,Hecker M,Wollscheid B,Sun Z,Moritz RL,Völker U,Schmidt F

更新日期：2015-11-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:Elite rice hybrids, when compared to their parental lines, exhibit increased yield and other favorable agronomical traits, such as pathogen- and water-stress resistances, which are described as heterosis, and the molecular mechanism of heterosis remains to be elucidated. Since genomic sequences of the paternal (9311) ...

journal_title：Proteomics

authors： Wang W,Meng B,Ge X,Song S,Yang Y,Yu X,Wang L,Hu S,Liu S,Yu J

更新日期：2008-11-01 00:00:00

abstract：:On 23 and 24 July, 2007, the Centre for Economic and Social Aspects of Genomics (CESAGen) held its first sociomics workshop at the Wellcome Trust Genome Campus, Hinxton, UK. The topic was transformation of knowledge production. Participants included social scientists together with those working on different elements o...

journal_title：Proteomics

authors： McNally R

更新日期：2008-01-01 00:00:00

abstract：:Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease typically characterized by a distribution of eczematous skin lesions with lichenification, pruritic excoriations, and dry skin with wide varieties of pathophysiologic aspects. Recently, AD was divided into extrinsic and intrinsic forms according t...

journal_title：Proteomics

authors： Park YD,Kim SY,Jang HS,Seo EY,Namkung JH,Park HS,Cho SY,Paik YK,Yang JM

更新日期：2004-11-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...

journal_title：Proteomics

authors： Sidahmed-Adrar N,Ottavi JF,Benzoubir N,Ait Saadi T,Bou Saleh M,Mauduit P,Guettier C,Desterke C,Le Naour F

更新日期：2019-11-01 00:00:00

abstract：:Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...

journal_title：Proteomics

authors： Allal AS,Kähne T,Reverdin AK,Lippert H,Schlegel W,Reymond MA

更新日期：2004-08-01 00:00:00

abstract：:Aberrant telomerase reactivation in differentiated cells represents a major event in oncogenic transformation. Recurrent somatic mutations in the human telomerase reverse transcriptase (TERT) promoter region, predominantly localized to two nucleotide positions, are highly prevalent in many cancer types. Both mutations...

journal_title：Proteomics

authors： Makowski MM,Willems E,Fang J,Choi J,Zhang T,Jansen PW,Brown KM,Vermeulen M

更新日期：2016-02-01 00:00:00

abstract：:We here present the jmzReader library: a collection of Java application programming interfaces (APIs) to parse the most commonly used peak list and XML-based mass spectrometry (MS) data formats: DTA, MS2, MGF, PKL, mzXML, mzData, and mzML (based on the already existing API jmzML). The library is optimized to be used i...

journal_title：Proteomics

authors： Griss J,Reisinger F,Hermjakob H,Vizcaíno JA

更新日期：2012-03-01 00:00:00