Proteomic Profile of Daphnia pulex using Data-Independent Acquisition Mass Spectrometry and Ion Mobility Separation.

abstract：:The Annual Spring workshop of the HUPO-PSI was this year held at the EMBL International Centre for Advanced Training (EICAT) in Heidelberg, Germany. Delegates briefly reviewed the successes of the group to date. These include the wide spread implementation of the molecular interaction data exchange formats, PSI-MI XML...

journal_title：Proteomics

authors： Orchard S,Albar JP,Deutsch EW,Eisenacher M,Vizcaíno JA,Hermjakob H

更新日期：2011-11-01 00:00:00

abstract：:Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (...

journal_title：Proteomics

authors： Huang QY,Huang HQ

更新日期：2011-09-01 00:00:00

abstract：:In order to proceed through their life cycle, protozoan parasites of the genus Leishmania cycle between sandflies and mammals. This change of environment correlates with the differentiation from the promastigote stage (insect form) to the amastigote stage (intracellular mammalian form). The molecular basis underlying ...

journal_title：Proteomics

authors： Bente M,Harder S,Wiesgigl M,Heukeshoven J,Gelhaus C,Krause E,Clos J,Bruchhaus I

更新日期：2003-09-01 00:00:00

abstract：:The field of biochemistry is currently faced with the enormous challenge of assigning functional significance to more than thirty thousand predicted protein products encoded by the human genome. In order to accomplish this daunting task, methods will be required that facilitate the global analysis of proteins in compl...

journal_title：Proteomics

authors： Patricelli MP,Giang DK,Stamp LM,Burbaum JJ

更新日期：2001-09-01 00:00:00

abstract：:Proteome analysis of human hepatocellular carcinoma tissues was conducted using two-dimensional difference gel electrophoresis coupled with mass spectrometry. Paired samples from the normal and tumor region of resected human liver were labeled with Cy3 and Cy5, respectively while the pooled standard sample was labeled...

journal_title：Proteomics

authors： Liang CR,Leow CK,Neo JC,Tan GS,Lo SL,Lim JW,Seow TK,Lai PB,Chung MC

更新日期：2005-05-01 00:00:00

abstract：:A method is introduced to evaluate protein concentrations using the height sum of all MALDI-MS peaks that unambiguously match theoretic tryptic peptide masses of the protein sought after. The method uses native chromatographic protein fractionation prior to digestion but does not require any depletion, labeling, deriv...

journal_title：Proteomics

authors： Bublitz R,Kreusch S,Ditze G,Schulze M,Cumme GA,Fischer C,Winter A,Hoppe H,Rhode H

更新日期：2006-07-01 00:00:00

abstract：:Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...

journal_title：Proteomics

authors： Tan KT,Ang SJ,Tsai SY

更新日期：2020-03-01 00:00:00

abstract：:The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...

journal_title：Proteomics

authors： Odom GJ,Ban Y,Colaprico A,Liu L,Silva TC,Sun X,Pico AR,Zhang B,Wang L,Chen X

更新日期：2020-11-01 00:00:00

abstract：:Since the proposal of the signal hypothesis on protein subcellular sorting, a number of computational analyses have been performed in this field. A typical example is the development of prediction algorithms for the subcellular localization sites of input protein sequences. In this review, we mainly focus on the biolo...

journal_title：Proteomics

authors： Imai K,Nakai K

更新日期：2010-11-01 00:00:00

abstract：:The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen prepa...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Nebendahl M,Janssen O,Petersen A

更新日期：2010-12-01 00:00:00

abstract：:Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum, specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been p...

journal_title：Proteomics

authors： Barkla BJ,Vera-Estrella R,Pantoja O

更新日期：2012-09-01 00:00:00

abstract：:Serine/threonine protein phosphatase 1 (PP1) regulates multiple cellular processes. Protein phosphorylation-dephosphorylation is largely altered during ischemia and subsequent reperfusion. The brain is particularly vulnerable to stress resulting from ischemia-reperfusion (IR), however, the acquisition of ischemic tole...

journal_title：Proteomics

authors： Cid C,Garcia-Bonilla L,Camafeita E,Burda J,Salinas M,Alcazar A

更新日期：2007-09-01 00:00:00

abstract：:Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-b...

journal_title：Proteomics

authors： Pimienta G,Heithoff DM,Rosa-Campos A,Tran M,Esko JD,Mahan MJ,Marth JD,Smith JW

更新日期：2019-03-01 00:00:00

abstract：:Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification....

journal_title：Proteomics

authors： Kieffer P,Dommes J,Hoffmann L,Hausman JF,Renaut J

更新日期：2008-06-01 00:00:00

abstract：:Aphids are major insect pests of cereal crops, acting as virus vectors as well as causing direct damage. The responses of wheat to infestation by cereal aphid (Sitobion avenae) were investigated in a proteomic analysis. Approximately, 500 protein spots were reproducibly detected in the extracts from leaves of wheat se...

journal_title：Proteomics

authors： Ferry N,Stavroulakis S,Guan W,Davison GM,Bell HA,Weaver RJ,Down RE,Gatehouse JA,Gatehouse AM

更新日期：2011-05-01 00:00:00

abstract：:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...

journal_title：Proteomics

authors： Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

更新日期：2006-12-01 00:00:00

abstract：:The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...

journal_title：Proteomics

authors： Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

更新日期：2019-11-01 00:00:00

abstract：:The prediction of adverse drug reactions (ADRs) has become increasingly important, due to the rising concern on serious ADRs that can cause drugs to fail to reach or stay in the market. We proposed a framework for predicting ADR profiles by integrating protein-protein interaction (PPI) networks with drug structures. W...

journal_title：Proteomics

authors： Huang LC,Wu X,Chen JY

更新日期：2013-01-01 00:00:00

abstract：:Trophoblastic cell lines are widely used in in vitro studies of placental function as a surrogate for primary trophoblasts. To date, no reference proteomics dataset exists to directly compare the shared and unique characteristics of these cells. Here, we performed comparative proteomic profiling of the BeWo and HTR8/S...

journal_title：Proteomics

authors： Szklanna PB,Wynne K,Nolan M,Egan K,Áinle FN,Maguire PB

更新日期：2017-05-01 00:00:00

abstract：:Protein quantification at proteome-wide scale is an important aim, enabling insights into fundamental cellular biology and serving to constrain experiments and theoretical models. While proteome-wide quantification is not yet fully routine, many datasets approaching proteome-wide coverage are becoming available throug...

journal_title：Proteomics

authors： Wang M,Herrmann CJ,Simonovic M,Szklarczyk D,von Mering C

更新日期：2015-09-01 00:00:00

abstract：:Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an ...

journal_title：Proteomics

authors： Schueder F,Unterauer EM,Ganji M,Jungmann R

更新日期：2020-10-08 00:00:00

abstract：:Spatial organisation of proteins according to their function plays an important role in the specificity of their molecular interactions. Emerging proteomics methods seek to assign proteins to sub-cellular locations by partial separation of organelles and computational analysis of protein abundance distributions among ...

journal_title：Proteomics

authors： Trotter MW,Sadowski PG,Dunkley TP,Groen AJ,Lilley KS

更新日期：2010-12-01 00:00:00

abstract：:Simple protein separation by 1DE is a widely used method to reduce sample complexity and to prepare proteins for mass spectrometric identification via in-gel digestion. While several automated solutions are available for in-gel digestion particularly of small cylindric gel plugs derived from 2D gels, the processing of...

journal_title：Proteomics

authors： Schmidt C,Hesse D,Raabe M,Urlaub H,Jahn O

更新日期：2013-05-01 00:00:00

abstract：:Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...

journal_title：Proteomics

authors： Erce MA,Pang CN,Hart-Smith G,Wilkins MR

更新日期：2012-02-01 00:00:00

abstract：:Scab, caused by Fusarium graminearum, is a serious spike disease in wheat. To identify proteins in resistant wheat cultivar Wangshuibai induced by F. graminearum infection, proteins extracted from spikes 6, 12 and 24 h after inoculation were separated by 2-DE. Thirty protein spots showing 3-fold change in abundance wh...

journal_title：Proteomics

authors： Wang Y,Yang L,Xu H,Li Q,Ma Z,Chu C

更新日期：2005-11-01 00:00:00

abstract：:Membrane proteins are key molecules in the cell, and are important targets for pharmaceutical drugs. Few three-dimensional structures of membrane proteins have been obtained, which makes computational prediction of membrane proteins crucial for studies of these key molecules. Here, seven membrane protein topology pred...

journal_title：Proteomics

authors： Fagerberg L,Jonasson K,von Heijne G,Uhlén M,Berglund L

更新日期：2010-03-01 00:00:00

abstract：:Aberrant telomerase reactivation in differentiated cells represents a major event in oncogenic transformation. Recurrent somatic mutations in the human telomerase reverse transcriptase (TERT) promoter region, predominantly localized to two nucleotide positions, are highly prevalent in many cancer types. Both mutations...

journal_title：Proteomics

authors： Makowski MM,Willems E,Fang J,Choi J,Zhang T,Jansen PW,Brown KM,Vermeulen M

更新日期：2016-02-01 00:00:00

abstract：:Helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. In addition H. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are a...

journal_title：Proteomics

authors： Hynes SO,McGuire J,Falt T,Wadström T

更新日期：2003-03-01 00:00:00

abstract：:Proteins extracted from leaf blades of rice plants infected with blast fungus, Magnaporthe grisea, were separated by two-dimensional polyacrylamide gel electrophoresis. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane, and 63 proteins were analyzed by a gas-phase protein sequencer. ...

journal_title：Proteomics

authors： Konishi H,Ishiguro K,Komatsu S

更新日期：2001-09-01 00:00:00

abstract：:Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...

journal_title：Proteomics

authors： Ebhardt HA,Sabidó E,Hüttenhain R,Collins B,Aebersold R

更新日期：2012-04-01 00:00:00