Abstract:
:Innovation in genomics, transcriptomics, and proteomics research has created a plethora of state-of-the-art techniques such as nucleic acid sequencing and mass-spectrometry-based proteomics with paramount impact in the life sciences. While current approaches yield quantitative abundance analysis of biomolecules on an almost routine basis, coupling this high content to spatial information in a single cell and tissue context is challenging. Here, current implementations of spatial omics are discussed and recent developments in the field of DNA-barcoded fluorescence microscopy are reviewed. Light is shed on the potential of DNA-based imaging techniques to provide a comprehensive toolbox for spatial genomics and transcriptomics and discuss current challenges, which need to be overcome on the way to spatial proteomics using high-resolution fluorescence microscopy.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Schueder F,Unterauer EM,Ganji M,Jungmann Rdoi
10.1002/pmic.201900368subject
Has Abstractpub_date
2020-10-08 00:00:00pages
e1900368eissn
1615-9853issn
1615-9861pub_type
杂志文章,评审相关文献
PROTEOMICS文献大全abstract::Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800935
更新日期:2009-05-01 00:00:00
abstract::Diurnal physiological acclimation regulated by a circadian system is an advantage for plant fitness. The circadian system is composed of a signal input, the clock and output pathways. Understanding the regulation mechanism of the output pathways remains a major challenge. Diurnal proteomic change reflects the state of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000065
更新日期:2011-01-01 00:00:00
abstract::Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200356
更新日期:2013-03-01 00:00:00
abstract::The activation of caspases is a critical event for the execution phase of programmed cell death. Caspases are highly specific in their ability to activate or inhibit many crucial proteins in the cell via site-specific cleavage. To date, more than 60 proteins have been shown to be substrates of one or more caspases in ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400979
更新日期:2004-11-01 00:00:00
abstract::In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons: Low price, Visibl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200690121
更新日期:2006-09-01 00:00:00
abstract::Tumor development and progression consist of a series of complex processes involving multiple changes in gene expression (Paolo et al. Physiol. Rev., 1993, 73, 161-195; Lance et al. Cell., 1991, 64, 327-336). Tumor cells acquire an invasive and metastatic phenotype that is the main cause of death for cancer patients. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401132
更新日期:2005-03-01 00:00:00
abstract::Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a 2-DE-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800636
更新日期:2009-03-01 00:00:00
abstract::Cofilin, an actin-binding protein, regulates the rate, nature and extent of assembly of the actin cytoskeleton. Native Phast gels show that the addition of cofilin to an actin-DNase I complex (74 kDa) results in the formation of a ternary complex of 94 kDa indicating an equimolar stoichiometry in the ternary complex. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200111)1:12<1513::aid-prot1513>3
更新日期:2001-12-01 00:00:00
abstract::The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900109
更新日期:2019-11-01 00:00:00
abstract::Lipid particles (LP) of all types of cells are a depot of neutral lipids. The present investigation deals with the isolation of LP from the yeast Yarrowia lipolytica and the characterization of their lipid and protein composition. Properties of LP varied depending on the carbon source. LP from glucose-grown cells reve...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500339
更新日期:2006-03-01 00:00:00
abstract::Acute kidney injury (AKI) is one of the more frequent and lethal pathological conditions seen in intensive care units. Currently available treatments are not totally effective but stem cell-based therapies are emerging as promising alternatives, especially the use of mesenchymal stromal cells (MSC), although the signa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300084
更新日期:2014-06-01 00:00:00
abstract::Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500137
更新日期:2006-01-01 00:00:00
abstract::Many tumors arising from epithelial tissues produce mucins, which readily come into contact with infiltrating cells in cancer tissues. MUC2 mucins were purified from the conditioned medium of a colorectal cancer cell line, LS180 cells. It is known that in cancer patients, the number of dendritic cells (DCs) is reduced...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800039
更新日期:2008-08-01 00:00:00
abstract::The present review deals with the use of combinatorial ligand libraries, composed by hexapeptides, in the capture and concentration of the low-abundance proteome. This method, first reported in 2005, is compared with other current methodologies aimed at exploring the "deep proteome", such as: depletion of high-abundan...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800389
更新日期:2009-03-01 00:00:00
abstract::Large-scale interaction studies contribute the largest fraction of protein interactions information in databases. However, co-purification of non-specific or indirect ligands, often results in data sets that are affected by a considerable number of false positives. For the fraction of interactions mediated by short li...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000030
更新日期:2011-01-01 00:00:00
abstract::Warm ischemia reperfusion (IR) injury of the liver is associated with changes in the expression and/or post-translational modification of numerous proteins. Only a few of these have been identified. We used 2-D DIGE to identify cytosolic proteins altered in the early stage of IR in an established rat model of segmenta...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100053
更新日期:2011-11-01 00:00:00
abstract::Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701114
更新日期:2008-08-01 00:00:00
abstract::Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry-based proteomics. Their common principle is to comprehensively measur...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900330
更新日期:2020-08-03 00:00:00
abstract::Protein interactions have been at the focus of computational biology in recent years. In particular, interest has come from two different communities--structural and systems biology. Here, we will discuss key systems and structural biology methods that have been used for analysis and prediction of protein-protein inte...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100597
更新日期:2012-05-01 00:00:00
abstract::The rice heterotrimeric G-protein complex, a guanine-nucleotide-dependent on-off switch, mediates vital cellular processes and responses to biotic and abiotic stress. Exchange of bound GDP (resting state) for GTP (active state) is spontaneous in plants including rice and thus there is no need for promoting guanine nuc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800385
更新日期:2019-05-01 00:00:00
abstract::Two new iodoacetamide-substituted cyanines, C3NIASO3 and C5NIASO3, were synthesized starting from hemicyanine and were utilized for labeling plasma proteins. Specificity, sensitivity and feasibility for SH residues was tested utilizing an equimolar mixture of standard proteins and with normal plasma. Oxidized plasma p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800254
更新日期:2009-01-01 00:00:00
abstract::Unconjugated bilirubin (UCB) is a powerful antioxidant and a modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop a physiological jaundice, in case of severe hyperbilirubinemia UCB may become neurotoxic causing severe long-term neuronal damages, also kno...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900579
更新日期:2010-04-01 00:00:00
abstract::Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. sc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500245
更新日期:2016-04-01 00:00:00
abstract::Separation and identification of hydrophobic membrane proteins is a major challenge in proteomics. Identification of such sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins by peptide mass fingerprinting (PMF) via matrix-assisted laser desorption/ionization-time of flight (MALDI-TO...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400851
更新日期:2004-12-01 00:00:00
abstract::Chinese tarantula, Chilobrachys jingzhao is one of the most venomous spiders in southern China and its venom is a mixture of various compounds with diversified biological activities. The proteome of C. jingzhao venom was analyzed by proteomic techniques. Proteins with molecular weight of over 10 kDa, indicated by gel-...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600785
更新日期:2007-06-01 00:00:00
abstract::A simple and rapid method for characterizing hydrophobic integral membrane proteins and its utility for membrane proteomics using microcapillary liquid chromatography coupled on-line with tandem mass spectrometry (microLC-MS/MS) is described. The present technique does not rely on the use of detergents, strong organic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300543
更新日期:2004-01-01 00:00:00
abstract::We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800201
更新日期:2008-09-01 00:00:00
abstract::The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200601019
更新日期:2007-09-01 00:00:00
abstract::Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000382
更新日期:2011-02-01 00:00:00
abstract::Recombinant human growth hormone (rhGH) is used for the treatment of several disorders. Structural integrity of rhGH is of critical importance for its clinical use and modifications thereof may act as markers in situations such as rhGH doping, as illegal rhGH-abuse in sports is of increasing interest. In the current s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500334
更新日期:2006-02-01 00:00:00