Impact of ArcA loss in Shewanella oneidensis revealed by comparative proteomics under aerobic and anaerobic conditions.

abstract：:Advanced glycation endproduct (AGE) formation is an important mechanism for protein deterioration during diabetic complications and ageing. The effects on AGE formation following dihydroxyacetone (DHA) stress were studied in two model organisms, the yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegan...

journal_title：Proteomics

authors： Molin M,Pilon M,Blomberg A

更新日期：2007-10-01 00:00:00

abstract：:Urinary microvesicles constitute a rich source of membrane-bound and intracellular proteins that may provide important clues of pathophysiological mechanisms in renal disease. In the current study, we analyzed and compared the proteome of urinary microvesicles from patients with idiopathic membranous nephropathy (iMN)...

journal_title：Proteomics

authors： Rood IM,Merchant ML,Wilkey DW,Zhang T,Zabrouskov V,van der Vlag J,Dijkman HB,Willemsen BK,Wetzels JF,Klein JB,Deegens JK

更新日期：2015-11-01 00:00:00

abstract：:During early stages of embryo development, the brain cavity is filled with embryonic cerebrospinal fluid (E-CSF), a complex fluid containing different protein fractions that contributes to the regulation of the survival, proliferation and neurogenesis of the neuroectodermal stem cells. Using 2-DE, protein sequencing a...

journal_title：Proteomics

authors： Parada C,Gato A,Aparicio M,Bueno D

更新日期：2006-01-01 00:00:00

abstract：:A proteomic analysis of a wild-type and of a phoB mutant showed that Vibrio cholerae expresses genes of two major regulons in response to phosphate starvation. The Pho regulon, expressed by the wild-type, allowed the cells to adapt to the new environment. Induction of the general stress regulon was mainly observed in ...

journal_title：Proteomics

authors： von Krüger WM,Lery LM,Soares MR,de Neves-Manta FS,Batista e Silva CM,Neves-Ferreira AG,Perales J,Bisch PM

更新日期：2006-03-01 00:00:00

abstract：:The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...

journal_title：Proteomics

authors： Lubelwana Hafver T,Wanichawan P,Manfra O,de Souza GA,Lunde M,Martinsen M,Louch WE,Sejersted OM,Carlson CR

更新日期：2017-09-01 00:00:00

abstract：:As an initial screen for novel markers of renal cancer and to minimise background heterogeneity, we have compared the within-patient profiles of serum samples from seven patients pre- and post-nephrectomy. Samples were depleted of six of the most abundant proteins using Agilent's multiple affinity removal system (MARS...

journal_title：Proteomics

authors： Vasudev NS,Ferguson RE,Cairns DA,Stanley AJ,Selby PJ,Banks RE

更新日期：2008-12-01 00:00:00

abstract：:To exploit advances in proteomics for drug discovery, high-throughout methods for target validation that directly address the cellular roles of proteins are required. To do this, we have characterized fluorophore-assisted light inactivation (FALI) which uses coherent or diffuse light targeted by fluorescein-labeled pr...

journal_title：Proteomics

authors： Beck S,Sakurai T,Eustace BK,Beste G,Schier R,Rudert F,Jay DG

更新日期：2002-03-01 00:00:00

abstract：:Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...

journal_title：Proteomics

authors： Hu S,Xie Y,Ramachandran P,Ogorzalek Loo RR,Li Y,Loo JA,Wong DT

更新日期：2005-04-01 00:00:00

abstract：:In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...

journal_title：Proteomics

authors： Grimplet J,Wheatley MD,Jouira HB,Deluc LG,Cramer GR,Cushman JC

更新日期：2009-05-01 00:00:00

abstract：:Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrom...

journal_title：Proteomics

authors： Nissum M,Kuhfuss S,Hauptmann M,Obermaier C,Sukop U,Wildgruber R,Weber G,Eckerskorn C,Malmström J

更新日期：2007-12-01 00:00:00

abstract：:The pilot phase of the Human Brain Proteome Project as a part of the Human Proteome Organisation has just been started. In two pilot studies, 18 different laboratories are analyzing mouse brains of three age stages and human brain autopsy versus biopsy material, respectively. The overall aim is to elucidate the portfo...

journal_title：Proteomics

authors： Blüggel M,Bailey S,Körting G,Stephan C,Reidegeld KA,Thiele H,Apweiler R,Hamacher M,Meyer HE

更新日期：2004-08-01 00:00:00

abstract：:Cryopreservation is widely used in many assisted conception units. Semen cryopreservation is the only proven method that offers many couples the chance to have children. However, spermatozoa are exposed to physical and chemical stressors during freezing and thawing that result in adverse changes in membrane lipid comp...

journal_title：Proteomics

authors： Yuan J

更新日期：2014-02-01 00:00:00

abstract：:We bring a "spectrum" of classical data mining and statistical analysis methods to bear on discrimination of two groups of spectra from 24 diseased and 17 normal patients. Our primary goal is to accurately estimate the generalizability of this small dataset. After an aggressive preprocessing step that reduces consider...

journal_title：Proteomics

authors： Neville P,Tan PY,Mann G,Wolfinger R

更新日期：2003-09-01 00:00:00

abstract：:The availability of completed genome sequences of several eukaryotic and prokaryotic species has shifted the focus towards the identification and characterization of all gene products that are expressed in a given organism. In order to cope with the huge amounts of data that have been provided by large-scale sequencin...

journal_title：Proteomics

authors： Auerbach D,Thaminy S,Hottiger MO,Stagljar I

更新日期：2002-06-01 00:00:00

abstract：:Lipid rafts are membrane microdomains of unique lipid composition that segregate proteins with poorly understood consequences for membrane organization. Identification of raft associated proteins could therefore provide novel insight into raft-dependent functions. Monocytes process antigens for presentation to T cells...

journal_title：Proteomics

authors： Li N,Mak A,Richards DP,Naber C,Keller BO,Li L,Shaw AR

更新日期：2003-04-01 00:00:00

abstract：:Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...

journal_title：Proteomics

authors： Davoodi J,Kelly J,Gendron NH,MacKenzie AE

更新日期：2007-06-01 00:00:00

abstract：:Until now, no data are available on the outer membrane (OM) proteome of Erwinia amylovora, a Gram-negative plant pathogen, causing fire blight in most of the members of the Rosaceae family. Since the OM forms the interface between the bacterial cell and its environment it is in direct contact with the host. Additional...

journal_title：Proteomics

authors： Holtappels M,Noben JP,Valcke R

更新日期：2016-09-01 00:00:00

abstract：:The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...

journal_title：Proteomics

authors： Blair BG,Wu X,Zahari MS,Mohseni M,Cidado J,Wong HY,Beaver JA,Cochran RL,Zabransky DJ,Croessmann S,Chu D,Toro PV,Cravero K,Pandey A,Park BH

更新日期：2015-01-01 00:00:00

abstract：:Phosphoproteomics can be defined as the comprehensive study of protein phosphorylation by identification of the phosphoproteins, exact mapping of the phosphorylation sites, quantification of phosphorylation, and eventually, revealing their biological function. Its place in today's research is vitally important to addr...

journal_title：Proteomics

authors： Kersten B,Agrawal GK,Iwahashi H,Rakwal R

更新日期：2006-10-01 00:00:00

abstract：:The effects of di(2-ethylhexyl) phthalate (DEHP) on proteins secreted by HepG2 cells were studied using a proteomic approach. HepG2 cells were exposed to various concentrations of DEHP (0, 2.5, 5, 10, 25, 50, 100, and 250 microM) for 24 or 48 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and c...

journal_title：Proteomics

authors： Choi S,Park SY,Jeong J,Cho E,Phark S,Lee M,Kwak D,Lim JY,Jung WW,Sul D

更新日期：2010-05-01 00:00:00

abstract：:Seed coat color is an important attribute determining consumption of soybean seeds. Soybean cultivar Mallikong (M) has yellow seed coat while its naturally mutated cultivar Mallikong mutant (MM), has brown colored seed coat. We used integrated proteomics and metabolomics approach to investigate the differences between...

journal_title：Proteomics

authors： Gupta R,Min CW,Kim SW,Wang Y,Agrawal GK,Rakwal R,Kim SG,Lee BW,Ko JM,Baek IY,Bae DW,Kim ST

更新日期：2015-05-01 00:00:00

abstract：:Antibody-based proteomics applied to tissue microarray (TMA) technology provides a very efficient means of visualizing and locating antigen expression in large collections of normal and pathological tissue samples. To characterize antigen expression on TMAs, the use of image analysis methods avoids the effects of huma...

journal_title：Proteomics

authors： Decaestecker C,Lopez XM,D'Haene N,Roland I,Guendouz S,Duponchelle C,Berton A,Debeir O,Salmon I

更新日期：2009-10-01 00:00:00

abstract：:Beneficial microbes have a positive impact on the productivity and fitness of the host plant. A better understanding of the biological impacts and underlying mechanisms by which the host derives these benefits will help to address concerns around global food production and security. The recent development of omics-bas...

journal_title：Proteomics

authors： Khatabi B,Gharechahi J,Ghaffari MR,Liu D,Haynes PA,McKay MJ,Mirzaei M,Salekdeh GH

更新日期：2019-08-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:Cancer cells maintain their intracellular ROS concentrations at required levels for their survival. Changes in ROS concentrations can regulate biochemical signaling mechanisms that control cell function. It has been demonstrated that ROS regulate the cellular events through redox regulation of redox-sensitive proteins...

journal_title：Proteomics

authors： Yuan K,Liu Y,Chen HN,Zhang L,Lan J,Gao W,Dou Q,Nice EC,Huang C

更新日期：2015-01-01 00:00:00

abstract：:Unconjugated bilirubin (UCB) is a powerful antioxidant and a modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop a physiological jaundice, in case of severe hyperbilirubinemia UCB may become neurotoxic causing severe long-term neuronal damages, also kno...

journal_title：Proteomics

authors： Deganuto M,Cesaratto L,Bellarosa C,Calligaris R,Vilotti S,Renzone G,Foti R,Scaloni A,Gustincich S,Quadrifoglio F,Tiribelli C,Tell G

更新日期：2010-04-01 00:00:00

abstract：:Proteins and peptides serve as biomarkers in the context of multiple pathologies. The hypothesis that protein or peptide biomarkers may also be of value in the context of the Covid-19 pandemic appears self-evident. Proteome based biomarkers are not expected to display significant added value in the detection of viral ...

journal_title：Proteomics

authors： Jankowski J

更新日期：2020-12-01 00:00:00

abstract：:SELDI-TOF-MS is rapidly gaining popularity as a screening tool for clinical applications of proteomics. Application of adequate statistical techniques in all the stages from measurement to information is obligatory. One of the statistical methods often used in proteomics is classification: the assignment of subjects t...

journal_title：Proteomics

authors： Hendriks MM,Smit S,Akkermans WL,Reijmers TH,Eilers PH,Hoefsloot HC,Rubingh CM,de Koster CG,Aerts JM,Smilde AK

更新日期：2007-10-01 00:00:00

abstract：:The von Hippel-Lindau (VHL) tumour suppressor gene plays a central role in development of clear cell renal cell carcinoma (RCC). Using a cell line pair generated from the VHL-defective RCC cell line UMRC2 by transfection with vector control or VHL (-/+VHL) and stable isotope labelling with amino acids in cell culture ...

journal_title：Proteomics

authors： Aggelis V,Craven RA,Peng J,Harnden P,Cairns DA,Maher ER,Tonge R,Selby PJ,Banks RE

更新日期：2009-04-01 00:00:00

abstract：:This paper proposes a novel profiling method for SELDI-TOF and MALDI-TOF MS data that integrates a novel peak detection method based on modified smoothed non-linear energy operator, correlation-based peak selection and Bayesian additive regression trees. The peak detection and classification performance of the propose...

journal_title：Proteomics

authors： He S,Li X,Viant MR,Yao X

更新日期：2009-09-01 00:00:00