Abstract:
:Cryopreservation is widely used in many assisted conception units. Semen cryopreservation is the only proven method that offers many couples the chance to have children. However, spermatozoa are exposed to physical and chemical stressors during freezing and thawing that result in adverse changes in membrane lipid composition, sperm motility, viability, and acrosome status. Wang et al. (Proteomics 2014, 14, 298-310) evaluate the protein content of freeze-thawed sperm samples relative to that of fresh sperm samples from the same normozoospermic donors. Four proteins are verified via Western blot and immunofluorescent staining, which are putatively involved in spermatozoon dysfunction. These marked differences demonstrated by Wang et al. suggest that dysfunctional spermatozoon after cryopreservation may be due to protein degradation and protein phosphorylation.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Yuan Jdoi
10.1002/pmic.201300564subject
Has Abstractpub_date
2014-02-01 00:00:00pages
155-6issue
2-3eissn
1615-9853issn
1615-9861journal_volume
14pub_type
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