Proteomic identification of differentially expressed plasma membrane proteins in renal cell carcinoma by stable isotope labelling of a von Hippel-Lindau transfectant cell line model.

abstract：:Low-density lipoprotein (LDL) is a major cholesterol carrier in human blood. Oxidations of apolipoprotein B-100 (apo B-100, LDL protein) could be proatherogenic and play critical roles in early stages of plaque formation in the arterial wall. The structure of apo B-100 is still poorly understood, partially due to its ...

journal_title：Proteomics

authors： Chakraborty S,Cai Y,Tarr MA

更新日期：2014-11-01 00:00:00

abstract：:A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differenti...

journal_title：Proteomics

authors： Winter D,Seidler J,Kugelstadt D,Derrer B,Kappes B,Lehmann WD

更新日期：2010-04-01 00:00:00

abstract：:Identification of differential proteomic responses to cadmium (Cd) would provide a means for better understanding the survival mechanisms of the earthworm, Eisenia fetida, living in a Cd-polluted environment, and the stress responses can be assessed by ecotoxiptoromics approaches. Extracts of whole earthworm collected...

journal_title：Proteomics

authors： Wang X,Chang L,Sun Z,Zhang Y,Yao L

更新日期：2010-12-01 00:00:00

abstract：:While metastasis is the primary cause of colorectal cancer (CRC) mortality, the molecular mechanisms underpinning it remains elusive. Metastasis is propagated through driver oncogene/suppressor gene mutations, accompanied by passenger mutations and underlying genomic instability. To understand cancer biology, a unifyi...

journal_title：Proteomics

authors： Ahn SB,Mohamedali A,Pascovici D,Adhikari S,Sharma S,Nice EC,Baker MS

更新日期：2019-11-01 00:00:00

abstract：:Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...

journal_title：Proteomics

authors： Kamita M,Mori T,Sakai Y,Ito S,Gomi M,Miyamoto Y,Harada A,Niida S,Yamada T,Watanabe K,Ono M

更新日期：2015-05-01 00:00:00

abstract：:The CpG motif of bacterial DNA (CpG-DNA) is a potent immunostimulating agent whose mechanism of action is not yet clear. Here, we used both DNA microarray and proteomic approaches to investigate the effects of oligodeoxynucleotides containing the CpG motif (CpG-ODN) on gene transcription and protein expression profile...

journal_title：Proteomics

authors： Kuo CC,Kuo CW,Liang CM,Liang SM

更新日期：2005-03-01 00:00:00

abstract：:SARS-CoV-2 infection results in a mild-to-moderate disease course in most patients, allowing outpatient self-care and quarantine. However, in approx. 10% of cases a two- or three-phasic critical disease course with starting from day 7 to 10 is observed. To facilitate and plan outpatient care, biomarkers prognosing suc...

journal_title：Proteomics

authors： Wendt R,Kalbitz S,Lübbert C,Kellner N,Macholz M,Schroth S,Ermisch J,Latosisnka A,Arnold B,Mischak H,Beige J,Metzger J

更新日期：2020-09-10 00:00:00

abstract：:Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...

journal_title：Proteomics

authors： Echan LA,Tang HY,Ali-Khan N,Lee K,Speicher DW

更新日期：2005-08-01 00:00:00

abstract：:The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...

journal_title：Proteomics

authors： Ihling C,Sinz A

更新日期：2005-05-01 00:00:00

abstract：:Aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. During human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. Here, we report changes in protein expression in the heads o...

journal_title：Proteomics

authors： Nunes AT,Brito NF,Oliveira DS,Araujo GD,Nogueira FC,Domont GB,Moreira MF,Moreira LM,Soares MR,Melo AC

更新日期：2016-10-01 00:00:00

abstract：:Bio-active peptides are involved in the regulation of most physiological processes in the body. Classical bio-active peptides (CBAPs) are cleaved from a larger precursor protein and stored in secretion vesicles from which they are released in the extracellular space. Recently, another non-classical type of bio-active ...

journal_title：Proteomics

authors： Budamgunta H,Olexiouk V,Luyten W,Schildermans K,Maes E,Boonen K,Menschaert G,Baggerman G

更新日期：2018-05-01 00:00:00

abstract：:2-D analysis of plant proteomes containing thousands of proteins has limited dynamic resolution because only abundant proteins can be detected. Proteomic assessment of the non-abundant proteins within seeds is difficult when 60-80% is storage proteins. Resolution can be improved through sample fractionation using sepa...

journal_title：Proteomics

authors： Krishnan HB,Oehrle NW,Natarajan SS

更新日期：2009-06-01 00:00:00

abstract：:FtsH is the only membrane-bound and essential protease in Escherichia coli. It is responsible for the degradation of regulatory proteins and enzymes such as the heat-shock sigma factor RpoH or LpxC, the key enzyme of lipopolysaccharide biosynthesis. To find new FtsH targets, we trapped substrates in E. coli cells from...

journal_title：Proteomics

authors： Arends J,Thomanek N,Kuhlmann K,Marcus K,Narberhaus F

更新日期：2016-12-01 00:00:00

abstract：:This study describes the gel-free phosphoproteomic analysis of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination of strong cation exchange and IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing over ...

journal_title：Proteomics

authors： Davanture M,Dumur J,Bataillé-Simoneau N,Campion C,Valot B,Zivy M,Simoneau P,Fillinger S

更新日期：2014-07-01 00:00:00

abstract：:Roots, leaves, and intermediate sections between roots and leaves (ISRL) of wheat seedlings show different physiological functions at the protein level. We performed the first integrative proteomic analysis of different tissues of the drought-tolerant wheat cultivar Hanxuan 10 (HX-10) and drought-sensitive cultivar Ch...

journal_title：Proteomics

authors： Hao P,Zhu J,Gu A,Lv D,Ge P,Chen G,Li X,Yan Y

更新日期：2015-05-01 00:00:00

abstract：:Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...

journal_title：Proteomics

authors： Yu H,Hackenbroch L,Meyer FRL,Reiser J,Razzazi-Fazeli E,Nöbauer K,Besenfelder U,Vogl C,Brem G,Mayrhofer C

更新日期：2019-03-01 00:00:00

abstract：:In a previous report we demonstrated protein kinase C (PKC)-mediated phosphorylation of the ligand-binding domain (LBD) of orphan nuclear receptor TR2. In this report, we provide the evidence of PKC-mediated phosphorylation of the DNA-binding domain (DBD) of TR2. Two PKC target sites were predicted within the DBD, at ...

journal_title：Proteomics

authors： Khan SA,Park SW,Huq MD,Wei LN

更新日期：2006-01-01 00:00:00

abstract：:Corynebacteria belong to the high G+C Gram-positive bacteria (Actinobacteria) and are closely related to Mycobacterium and Nocardia species. The best investigated member of this group of almost seventy species is Corynebacterium glutamicum, a soil bacterium isolated in 1957, which is used for the industrial production...

journal_title：Proteomics

authors： Poetsch A,Haussmann U,Burkovski A

更新日期：2011-08-01 00:00:00

abstract：:The protein components of urine are useful indicators of renal function and human health in general. Urine samples are easily attainable making them ideal substrates for biomarker research. Analysis of the urine proteome however, has been hindered by the great variability of the urine specimens, and the presence of va...

journal_title：Proteomics

authors： Zerefos PG,Vougas K,Dimitraki P,Kossida S,Petrolekas A,Stravodimos K,Giannopoulos A,Fountoulakis M,Vlahou A

更新日期：2006-08-01 00:00:00

abstract：:3Z-3-[(1H-pyrrol-2-yl)-methylidene]-1-(1-piperidinylmethyl)-1,3-2H-indol-2-one (Z24), a synthetic anti-angiogenic compound, inhibits the growth and metastasis of certain tumors. Previous works have shown that Z24 induces hepatotoxicity in rodents. We examined the hepatotoxic mechanism of Z24 at the protein level and l...

journal_title：Proteomics

authors： Wang Y,Yang B,Wu C,Zheng Z,Yuan Y,Hu Z,Ma H,Li S,Liao M,Wang Q

更新日期：2010-08-01 00:00:00

abstract：:Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

更新日期：2009-07-01 00:00:00

abstract：:Menthol is a naturally occurring cyclic monoterpene used in oral hygiene products, confectionary, pharmaceuticals, cosmetics, pesticides, and as a flavoring agent. In the present study, we analyzed the differentially expressing proteome in L-menthol-treated Caco-2 cell line as it was found to inhibit cell proliferatio...

journal_title：Proteomics

authors： Faridi U,Sisodia BS,Shukla AK,Shukla RK,Darokar MP,Dwivedi UN,Shasany AK

更新日期：2011-05-01 00:00:00

abstract：:Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...

journal_title：Proteomics

authors： Guergues J,Zhang P,Liu B,Stevens SM Jr

更新日期：2019-06-01 00:00:00

abstract：:Clostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate-active enzymes were identified, and their modular structures and activities ...

journal_title：Proteomics

authors： Blouzard JC,Coutinho PM,Fierobe HP,Henrissat B,Lignon S,Tardif C,Pagès S,de Philip P

更新日期：2010-02-01 00:00:00

abstract：:In higher plants many different genes encode Lhcb proteins that belong to a highly conserved protein family. Evolutionary conservation of this genetic redundancy suggests that individual gene products play different roles in light harvesting and photoprotection depending on environmental conditions. We have tested the...

journal_title：Proteomics

authors： Caffarri S,Frigerio S,Olivieri E,Righetti PG,Bassi R

更新日期：2005-02-01 00:00:00

abstract：:The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...

journal_title：Proteomics

authors： Blair BG,Wu X,Zahari MS,Mohseni M,Cidado J,Wong HY,Beaver JA,Cochran RL,Zabransky DJ,Croessmann S,Chu D,Toro PV,Cravero K,Pandey A,Park BH

更新日期：2015-01-01 00:00:00

abstract：:High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...

journal_title：Proteomics

authors： Majoul T,Bancel E,Triboï E,Ben Hamida J,Branlard G

更新日期：2003-02-01 00:00:00

abstract：:Quantitative proteomic analysis using stable isotope labeling with amino acids in cell culture (SILAC), as metabolic labeling with MS, has been used as an excellent technique to measure relative abundance change in proteins and post-transitional modifications. Since its development in 2002, SILAC has proven to have un...

journal_title：Proteomics

authors： Jang WE,Kim MS

更新日期：2018-03-01 00:00:00

abstract：:The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...

journal_title：Proteomics

authors： Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh J

更新日期：2006-11-01 00:00:00

abstract：:Cancer cells maintain their intracellular ROS concentrations at required levels for their survival. Changes in ROS concentrations can regulate biochemical signaling mechanisms that control cell function. It has been demonstrated that ROS regulate the cellular events through redox regulation of redox-sensitive proteins...

journal_title：Proteomics

authors： Yuan K,Liu Y,Chen HN,Zhang L,Lan J,Gao W,Dou Q,Nice EC,Huang C

更新日期：2015-01-01 00:00:00