Analysis of earthworm Eisenia fetida proteomes during cadmium exposure: An ecotoxicoproteomics approach.

abstract：:A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differenti...

journal_title：Proteomics

authors： Winter D,Seidler J,Kugelstadt D,Derrer B,Kappes B,Lehmann WD

更新日期：2010-04-01 00:00:00

abstract：:The community working on model organisms is growing steadily and the number of model organisms for which proteome data are being generated is continuously increasing. To standardize efforts and to make optimal use of proteomics data acquired from model organisms, a new Human Proteome Organisation (HUPO) initiative on ...

journal_title：Proteomics

authors： Jones AM,Aebersold R,Ahrens CH,Apweiler R,Baerenfaller K,Baker M,Bendixen E,Briggs S,Brownridge P,Brunner E,Daube M,Deutsch EW,Grossniklaus U,Heazlewood J,Hengartner MO,Hermjakob H,Jovanovic M,Lawless C,Lochnit G,Ma

更新日期：2012-02-01 00:00:00

abstract：:A two-dimensional liquid-phase separation scheme coupled with mass spectrometry (MS) is presented for proteomic analysis of cell lysates from normal and malignant breast epithelial cell lines. Liquid-phase separations consist of isoelectric focusing as the first dimension and nonporous silica reverse-phase high-perfor...

journal_title：Proteomics

authors： Hamler RL,Zhu K,Buchanan NS,Kreunin P,Kachman MT,Miller FR,Lubman DM

更新日期：2004-03-01 00:00:00

abstract：:The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...

journal_title：Proteomics

authors： González-Camacho F,Medina FJ

更新日期：2004-02-01 00:00:00

abstract：:The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...

journal_title：Proteomics

authors： Jorba N,Juarez S,Torreira E,Gastaminza P,Zamarreño N,Albar JP,Ortín J

更新日期：2008-05-01 00:00:00

abstract：:Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...

journal_title：Proteomics

authors： Kunec D,Nanduri B,Burgess SC

更新日期：2009-05-01 00:00:00

abstract：:Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, we provide a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson-Golabi-Behmel Syndrome (SGBS) cells. The SGBS cells are a...

journal_title：Proteomics

authors： Kalkhof S,Krieg L,Büttner P,Wabitsch M,Küntzel C,Friebe D,Landgraf K,Hanschkow M,Schubert K,Kiess W,Krohn K,Blüher M,von Bergen M,Körner A

更新日期：2020-05-08 00:00:00

abstract：:Two-dimensional electrophoresis is a widely used method for separating a large number of proteins from complex protein mixtures and for revealing differential patterns of protein expressions. In the computer-assisted proteome research, the comparison of protein separation profiles involves several heuristic steps, ran...

journal_title：Proteomics

authors： Salmi J,Aittokallio T,Westerholm J,Griese M,Rosengren A,Nyman TA,Lahesmaa R,Nevalainen O

更新日期：2002-11-01 00:00:00

abstract：:Warm ischemia reperfusion (IR) injury of the liver is associated with changes in the expression and/or post-translational modification of numerous proteins. Only a few of these have been identified. We used 2-D DIGE to identify cytosolic proteins altered in the early stage of IR in an established rat model of segmenta...

journal_title：Proteomics

authors： Wilson CH,Zeile S,Chataway T,Nieuwenhuijs VB,Padbury RT,Barritt GJ

更新日期：2011-11-01 00:00:00

abstract：:It becomes increasingly clear that separation of pure cell populations provides a uniquely sensitive and accurate approach to protein profiling in biological systems and opens up a new area for proteomic analysis. The method we described could simultaneously isolate population of hepatocytes (HCs), hepatic stellate ce...

journal_title：Proteomics

authors： Liu W,Hou Y,Chen H,Wei H,Lin W,Li J,Zhang M,He F,Jiang Y

更新日期：2011-09-01 00:00:00

abstract：:Secreted proteins play a pivotal role in cellular functions. To better understand malignant behavior, we adapted stable isotopic labeling with amino acids in cell culture technology to identify and quantify proteins differentially released into the extracellular media by a pair of normal and malignant breast-cancer ce...

journal_title：Proteomics

authors： Liang X,Huuskonen J,Hajivandi M,Manzanedo R,Predki P,Amshey JR,Pope RM

更新日期：2009-01-01 00:00:00

abstract：:Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...

journal_title：Proteomics

authors： ten Have S,Boulon S,Ahmad Y,Lamond AI

更新日期：2011-03-01 00:00:00

abstract：:Adipogenesis is an important aspect of energy homeostasis. Here we have used a differential proteome mapping strategy to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3 L1 preadipocytes. Two-dimensional gel electrophoresis analysis identified 8 proteins that are induc...

journal_title：Proteomics

authors： Choi KL,Wang Y,Tse CA,Lam KS,Cooper GJ,Xu A

更新日期：2004-06-01 00:00:00

abstract：:Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum pr...

journal_title：Proteomics

authors： Nicholas B,Skipp P,Mould R,Rennard S,Davies DE,O'Connor CD,Djukanović R

更新日期：2006-08-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. Miniaturization of IMAC column is essential for the analysis of a small amount of sample. Nanoscale IMAC column was prepared by chemical modification of...

journal_title：Proteomics

authors： Feng S,Pan C,Jiang X,Xu S,Zhou H,Ye M,Zou H

更新日期：2007-02-01 00:00:00

abstract：:Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such a...

journal_title：Proteomics

authors： Banfi C,Brioschi M,Wait R,Begum S,Gianazza E,Fratto P,Polvani G,Vitali E,Parolari A,Mussoni L,Tremoli E

更新日期：2006-03-01 00:00:00

abstract：:Apoptosis contributes to cell death after cerebral ischaemia. A quantitative proteomics approach has been employed to define alterations in protein levels in apoptosis induced with staurosporine (STS). Human neuroblastoma derived SH-SY5Y cells were treated with STS (500 nM for 6 h) to induce apoptosis. Quantitative 2-...

journal_title：Proteomics

authors： Short DM,Heron ID,Birse-Archbold JL,Kerr LE,Sharkey J,McCulloch J

更新日期：2007-09-01 00:00:00

abstract：:The hypothalamus is the central regulatory unit that balances a number of body functions including metabolic rate, hunger, and satiety signals. Hypothalamic neurons monitor and respond to alterations of circulating nutrients and hormones that reflect the peripheral energy status. These extracellular signals are integr...

journal_title：Proteomics

authors： Kuhla B,Kuhla S,Rudolph PE,Albrecht D,Metges CC

更新日期：2007-10-01 00:00:00

abstract：:Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/cGMP (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecul...

journal_title：Proteomics

authors： Zhang G,Wong YH,Zhang Y,He LS,Xu Y,Qian PY

更新日期：2015-11-01 00:00:00

abstract：:Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...

journal_title：Proteomics

authors： Liu T,Qian WJ,Chen WN,Jacobs JM,Moore RJ,Anderson DJ,Gritsenko MA,Monroe ME,Thrall BD,Camp DG 2nd,Smith RD

更新日期：2005-04-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry-based proteomics. Their common principle is to comprehensively measur...

journal_title：Proteomics

authors： Mann M

更新日期：2020-08-03 00:00:00

abstract：:Aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. During human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. Here, we report changes in protein expression in the heads o...

journal_title：Proteomics

authors： Nunes AT,Brito NF,Oliveira DS,Araujo GD,Nogueira FC,Domont GB,Moreira MF,Moreira LM,Soares MR,Melo AC

更新日期：2016-10-01 00:00:00

abstract：:Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...

journal_title：Proteomics

authors： Tan KT,Ang SJ,Tsai SY

更新日期：2020-03-01 00:00:00

abstract：:Aluminum (Al) toxicity is a major constraint for plant root development and growth as well as crop yield in acidic soils, which constitute approximately 40% of the potentially arable lands worldwide. The mechanisms of Al tolerance in plants are not well understood. As a whole systems approach, proteomic techniques hav...

journal_title：Proteomics

authors： Zheng L,Lan P,Shen RF,Li WF

更新日期：2014-03-01 00:00:00

abstract：:Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...

journal_title：Proteomics

authors： Nguyen-Kim H,San Clemente H,Balliau T,Zivy M,Dunand C,Albenne C,Jamet E

更新日期：2016-02-01 00:00:00

abstract：:The sample preparation procedures established for Prorocentrum triestinum were adapted to cover both thecate and athecate dinoflagellates. Further, whether trichloroacetic acid (TCA) precipitation can be used to fix and preserve the harmful or nuisance species from local waters that they infest was tested. Optimized t...

journal_title：Proteomics

authors： Chan LL,Hodgkiss IJ,Lu S,Lo SC

更新日期：2004-01-01 00:00:00

abstract：:An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to F...

journal_title：Proteomics

authors： Noga MJ,Lewandowski JJ,Suder P,Silberring J

更新日期：2005-11-01 00:00:00

abstract：:Cyanobacteria are oxygenic photosynthetic prokaryotes and play a crucial role in the Earth's carbon and nitrogen cycles. The photoautotrophic cyanobacterium Anabaena sp. PCC 7120 has the ability to fix atmospheric nitrogen in heterocysts and produce hydrogen as a byproduct through a nitrogenase. In order to improve hy...

journal_title：Proteomics

authors： Kourpa K,Manarolaki E,Lyratzakis A,Strataki V,Rupprecht F,Langer JD,Tsiotis G

更新日期：2019-10-01 00:00:00

abstract：:Pathogenic bacteria excrete a variety of virulence factors into extracellular medium and to the cell surface which have essential roles in the colonization and insurrection of the host cells, and thus reflect the degree of bacterial pathogenicity. For the exploration of virulence factors expressed in the secreted prot...

journal_title：Proteomics

authors： Pocsfalvi G,Cacace G,Cuccurullo M,Serluca G,Sorrentino A,Schlosser G,Blaiotta G,Malorni A

更新日期：2008-06-01 00:00:00