Increased expression of peroxiredoxin 1 and identification of a novel lipid-metabolizing enzyme in the early phase of liver ischemia reperfusion injury.

abstract：:Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood or bile contaminatio...

journal_title：Proteomics

authors： Zhou L,Lu Z,Yang A,Deng R,Mai C,Sang X,Faber KN,Lu X

更新日期：2007-04-01 00:00:00

abstract：:Proteolytic modification of components of the extracellular milieu by metalloproteinases plays important roles in the regulation of multiple cellular and physiological processes and pathological conditions. ADAMTS1 is a secreted enzyme of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) fami...

journal_title：Proteomics

authors： Canals F,Colomé N,Ferrer C,Plaza-Calonge Mdel C,Rodríguez-Manzaneque JC

更新日期：2006-04-01 00:00:00

abstract：:We report on a novel strategy for identification of specific sulfation motifs in chondroitin/dermatan sulfate (CS/DS) chain derived from decorin (Dcn), based on enzyme cleavage and multistage MS (MS(n)). Released CS/DS chains were digested with chondroitin B and in parallel with AC I lyases to obtain oligosaccharides ...

journal_title：Proteomics

authors： Zamfir AD,Flangea C,Sisu E,Serb AF,Dinca N,Bruckner P,Seidler DG

更新日期：2009-07-01 00:00:00

abstract：:The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...

journal_title：Proteomics

authors： Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

更新日期：2012-06-01 00:00:00

abstract：:MALDI-MS is an extremely flexible technique and can be synergistically used in conjunction with established bioanalytical methods such as PAGE or SPR. To that end, slight modifications on the sample target plate may be necessary. Those can involve the use of hydrophobic coatings for improved sample deposition and desa...

journal_title：Proteomics

authors： König S

更新日期：2008-02-01 00:00:00

abstract：:Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...

journal_title：Proteomics

authors： Kamita M,Mori T,Sakai Y,Ito S,Gomi M,Miyamoto Y,Harada A,Niida S,Yamada T,Watanabe K,Ono M

更新日期：2015-05-01 00:00:00

abstract：:High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...

journal_title：Proteomics

authors： Hörmann S,Scheyhing C,Behr J,Pavlovic M,Ehrmann M,Vogel RF

更新日期：2006-03-01 00:00:00

abstract：:The gut epithelium formed between an organism and the environment plays an essential role in host-microbe interactions, yet remains one of the least characterized mammalian tissues. Especially the membrane proteins, which are critical to bacterial adhesion, are understudied, because these proteins are low in abundance...

journal_title：Proteomics

authors： Hesselager MO,Codrea MC,Bendixen E

更新日期：2015-07-01 00:00:00

abstract：:Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...

journal_title：Proteomics

authors： Finehout EJ,Cantor JR,Lee KH

更新日期：2005-06-01 00:00:00

abstract：:We here present the jmzReader library: a collection of Java application programming interfaces (APIs) to parse the most commonly used peak list and XML-based mass spectrometry (MS) data formats: DTA, MS2, MGF, PKL, mzXML, mzData, and mzML (based on the already existing API jmzML). The library is optimized to be used i...

journal_title：Proteomics

authors： Griss J,Reisinger F,Hermjakob H,Vizcaíno JA

更新日期：2012-03-01 00:00:00

abstract：:In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes ...

journal_title：Proteomics

authors： Laria AE,Messineo S,Arcidiacono B,Varano M,Chiefari E,Semple RK,Rocha N,Russo D,Cuda G,Gaspari M,Brunetti A,Foti DP

更新日期：2018-04-01 00:00:00

abstract：:Plants exhibit resistance against incompatible pathogens, via localized and systemic responses as part of an integrated defense mechanism. To study the compatible and incompatible interactions between rice and bacteria, a proteomic approach was applied. Rice cv. Java 14 seedlings were inoculated with compatible (Xo743...

journal_title：Proteomics

authors： Mahmood T,Jan A,Kakishima M,Komatsu S

更新日期：2006-11-01 00:00:00

abstract：:FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...

journal_title：Proteomics

authors： Gattiker A,Bienvenut WV,Bairoch A,Gasteiger E

更新日期：2002-10-01 00:00:00

abstract：:Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are an important class of glycoproteins that are tethered to the surface of mammalian cells via the lipid GPI. GPI-APs have been implicated in many important cellular functions including cell adhesion, cell signaling, and immune regulation. Proteomic identificat...

journal_title：Proteomics

authors： Cortes LK,Vainauskas S,Dai N,McClung CM,Shah M,Benner JS,Corrêa IR Jr,VerBerkmoes NC,Taron CH

更新日期：2014-11-01 00:00:00

abstract：:Niemann-Pick disease, type C1 (NPC1) is a fatal, autosomal recessive, neurodegenerative disorder caused by mutations in the NPC1 gene. As a result of the genetic defect, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal/lysosomal system causing both visceral and neurological def...

journal_title：Proteomics

authors： Pergande MR,Serna-Perez F,Mohsin SB,Hanek J,Cologna SM

更新日期：2019-09-01 00:00:00

abstract：:The accurate and precise absolute abundance of proteins can be determined using mass spectrometry by spiking the sample with stable isotope-labeled standards. In this study, we developed a strategy of hierarchical use of peptide-concatenated standards (PCSs) to quantify more proteins over a wider dynamic range. Multip...

journal_title：Proteomics

authors： Kito K,Okada M,Ishibashi Y,Okada S,Ito T

更新日期：2016-05-01 00:00:00

abstract：:Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such a...

journal_title：Proteomics

authors： Banfi C,Brioschi M,Wait R,Begum S,Gianazza E,Fratto P,Polvani G,Vitali E,Parolari A,Mussoni L,Tremoli E

更新日期：2006-03-01 00:00:00

abstract：:Immune proteomics is an increasingly powerful tool for the investigation of the adaptive immune response to natural encounters between micro-organisms and their hosts. The versatile species Staphylococcus aureus serves to illustrate how these techniques can be employed to appreciate the complexity and diversity of the...

journal_title：Proteomics

authors： Bröker BM,van Belkum A

更新日期：2011-08-01 00:00:00

abstract：:Aberrant telomerase reactivation in differentiated cells represents a major event in oncogenic transformation. Recurrent somatic mutations in the human telomerase reverse transcriptase (TERT) promoter region, predominantly localized to two nucleotide positions, are highly prevalent in many cancer types. Both mutations...

journal_title：Proteomics

authors： Makowski MM,Willems E,Fang J,Choi J,Zhang T,Jansen PW,Brown KM,Vermeulen M

更新日期：2016-02-01 00:00:00

abstract：:The activation of caspases is a critical event for the execution phase of programmed cell death. Caspases are highly specific in their ability to activate or inhibit many crucial proteins in the cell via site-specific cleavage. To date, more than 60 proteins have been shown to be substrates of one or more caspases in ...

journal_title：Proteomics

authors： Lee AY,Park BC,Jang M,Cho S,Lee DH,Lee SC,Myung PK,Park SG

更新日期：2004-11-01 00:00:00

abstract：:The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...

journal_title：Proteomics

authors： Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

更新日期：2013-11-01 00:00:00

abstract：:Menthol is a naturally occurring cyclic monoterpene used in oral hygiene products, confectionary, pharmaceuticals, cosmetics, pesticides, and as a flavoring agent. In the present study, we analyzed the differentially expressing proteome in L-menthol-treated Caco-2 cell line as it was found to inhibit cell proliferatio...

journal_title：Proteomics

authors： Faridi U,Sisodia BS,Shukla AK,Shukla RK,Darokar MP,Dwivedi UN,Shasany AK

更新日期：2011-05-01 00:00:00

abstract：:Normal sera contain a large number of naturally occurring autoantibodies which can mask important disease-associated ones. Western blotting has evolved as the most important tool to demonstrate autoantibodies in autoimmune diseases, because of its ability to simultaneous screening for a wide spectrum of different anti...

journal_title：Proteomics

authors： Grus FH,Joachim SC,Pfeiffer N

更新日期：2003-06-01 00:00:00

abstract：:Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...

journal_title：Proteomics

authors： Schuemie M,Chichester C,Lisacek F,Coute Y,Roes PJ,Sanchez JC,Kors J,Mons B

更新日期：2007-03-01 00:00:00

abstract：:By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...

journal_title：Proteomics

authors： Medjahed D,Luke BT,Tontesh TS,Smythers GW,Munroe DJ,Lemkin PF

更新日期：2003-08-01 00:00:00

abstract：:Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...

journal_title：Proteomics

authors： Echan LA,Tang HY,Ali-Khan N,Lee K,Speicher DW

更新日期：2005-08-01 00:00:00

abstract：:We have combined high-resolution two-dimensional (2-D) gel electrophoresis and mass spectrometry with the aim of identifying proteins represented in the 2-D gel database of the wing imaginal discs of Drosophila melanogaster. First, we obtained a high-resolution 2-D gel pattern of [35S]methionine + [35S]cysteine-labele...

journal_title：Proteomics

authors： Alonso J,Santarén JF

更新日期：2005-02-01 00:00:00

abstract：:Staphylococcus aureus is an opportunistic human pathogen, which can cause life-threatening disease. Proteome analyses of the bacterium can provide new insights into its pathophysiology and important facets of metabolic adaptation and, thus, aid the recognition of targets for intervention. However, the value of such pr...

journal_title：Proteomics

authors： Depke M,Michalik S,Rabe A,Surmann K,Brinkmann L,Jehmlich N,Bernhardt J,Hecker M,Wollscheid B,Sun Z,Moritz RL,Völker U,Schmidt F

更新日期：2015-11-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:Biological membranes are compartmentalized into microdomains that exhibit particular lipid and protein compositions. Membrane microdomains, such as tetraspanin-enriched microdomains and lipid rafts, have been suggested to play a role in a variety of physiological and pathological processes. Therefore, the characteriza...

journal_title：Proteomics

authors： Le Naour F,André M,Boucheix C,Rubinstein E

更新日期：2006-12-01 00:00:00