Abstract:
:Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood or bile contamination, were collected from patients with pancreatic cancer (n = 5), benign pancreatic diseases (n = 6), or cholelithiasis (n = 3) during endoscopic retrograde cholangiopancreatography (ERCP). After ultramembrane centrifugation sample preparation, pancreatic juice proteins were separated by 2-DE and identified by MALDI-TOF-MS. A 2-DE dataset of pancreatic juice from patients with cholelithiasis was established, consisting of 76 protein spots representing 22 different proteins. Disease-associated obstruction of the pancreatic duct strongly effected the protein composition of pancreatic juice. Concurrently, pancreatic juice from patients with pancreatic cancer was compared to nonmalignant controls with comparable obstruction of pancreatic ducts. Seven protein spots were identified that consistently appeared at changed levels in pancreatic juice from patients with pancreatic cancer. In conclusion, comparative proteomic analysis of human pancreatic juice can be used to identify biomarkers of pancreatic cancer.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Zhou L,Lu Z,Yang A,Deng R,Mai C,Sang X,Faber KN,Lu Xdoi
10.1002/pmic.200600086subject
Has Abstractpub_date
2007-04-01 00:00:00pages
1345-55issue
8eissn
1615-9853issn
1615-9861journal_volume
7pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400132
更新日期:2015-01-01 00:00:00
abstract::Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either (13) C-naphthalene or (13) C-fluorene ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200569
更新日期:2013-10-01 00:00:00
abstract::In an accompanying study (in this issue, DOI 10.1002/pmic.200402044), we have characterised the proteome of Sca-1(+) progenitor cells, which may function as precursors of vascular smooth muscle cells (SMCs). In the present study, we have analysed and mapped protein expression in aortic SMCs of mice, using 2-DE, MALDI-...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402045
更新日期:2005-11-01 00:00:00
abstract::Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated software, named IQuant, which integrates a postprocessing tool of protein identification and advanced statistical algorithms to process the MS/MS signals generated f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300361
更新日期:2014-10-01 00:00:00
abstract::Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700588
更新日期:2008-02-01 00:00:00
abstract::Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300854
更新日期:2004-08-01 00:00:00
abstract::Mulberry dwarf (MD) is a serious infectious disease of mulberry caused by phytoplasma. Infection with MD phytoplasma results in stress phenotypes of yellowing, phyllody, stunting, proliferation, and witches' broom. Physiological and biochemical analysis has shown that infection with MD phytoplasma causes an increase i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900012
更新日期:2009-12-01 00:00:00
abstract::Metabolite flux to UDP-GlcNAc and Golgi N-glycan biosynthesis regulates surface residency of glycoprotein receptors and transporters, and thus sensitivities of cells to extracellular cues. Salvage of GlcNAc increases UDP-GlcNAc and branching of N-glycans progressively, but displays an optimum for cell proliferation an...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800208
更新日期:2008-08-01 00:00:00
abstract::MALDI-MS is an extremely flexible technique and can be synergistically used in conjunction with established bioanalytical methods such as PAGE or SPR. To that end, slight modifications on the sample target plate may be necessary. Those can involve the use of hydrophobic coatings for improved sample deposition and desa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700782
更新日期:2008-02-01 00:00:00
abstract::A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500721
更新日期:2006-05-01 00:00:00
abstract::Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700139
更新日期:2007-08-01 00:00:00
abstract::Renal cell carcinoma (RCC) originates in the renal cortex. It accounts for 2-3 percent of all cancers occurring in adults and it is characterised by lack of early clinical manifestations, unpredictable outcome, and absence of effective treatment modalities except early surgery. RCC comprises a heterogeneous group of t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200110)1:10<1288::AID-PROT1288>3
更新日期:2001-10-01 00:00:00
abstract::Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500129
更新日期:2006-03-01 00:00:00
abstract::Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum, specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200152
更新日期:2012-09-01 00:00:00
abstract::Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400442
更新日期:2015-05-01 00:00:00
abstract::Recently, we introduced a novel proteomics method employing a metalloendopeptidase with Lys-N specificity to produce proteolytic peptides. Fragmentation spectra generated by electron transfer dissociation, for a large proportion of the Lys-N proteolytic peptides, were found to be dominated by extensive series of c-typ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900405
更新日期:2010-03-01 00:00:00
abstract::When the p-value is set at <0.05 in statistical group comparisons, a 5% rate of "false significant" results is expected. In order to test the reliability of our 2-DE method, we loaded each of 24 gels with equal-sized samples (200 mug protein from pooled rat brain, pH 4-7, stained with ruthenium fluorescent stain for v...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701074
更新日期:2008-05-01 00:00:00
abstract::The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200401015
更新日期:2005-02-01 00:00:00
abstract::MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200501332
更新日期:2006-01-01 00:00:00
abstract::Suberoylanilide hydroxamic acid (SAHA) is a potent histone deacetylase (HDAC) inhibitor. Inhibitors of HDACs are used in cancer therapy based on the role HDACs play in transcription by regulating chromatin compaction and non-histone proteins such as transcription factors. Profiling of HDAC expression is of interest in...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000717
更新日期:2011-10-01 00:00:00
abstract::While metastasis is the primary cause of colorectal cancer (CRC) mortality, the molecular mechanisms underpinning it remains elusive. Metastasis is propagated through driver oncogene/suppressor gene mutations, accompanied by passenger mutations and underlying genomic instability. To understand cancer biology, a unifyi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900026
更新日期:2019-11-01 00:00:00
abstract::Proteins determine the quality of barley in malting and brewing end-uses. In this regard, water-soluble barley proteins play a major role in the formation, stability, and texture of head foams. Our objective was to survey the barley seed proteins that could be involved in the foaming properties of beer. Therefore, two...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401153
更新日期:2005-07-01 00:00:00
abstract::The use of anthrax spores as a bioweapon has spurred efforts aimed at identifying key proteins expressed in Bacillus anthracis. Because spore germination and outgrowth occur prior to and are required for disease manifestations, blocking germination and early outgrowth with novel vaccines or inhibitors targeting critic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400831
更新日期:2004-09-01 00:00:00
abstract::Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry-based proteomics. Their common principle is to comprehensively measur...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900330
更新日期:2020-08-03 00:00:00
abstract::The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400342
更新日期:2015-01-01 00:00:00
abstract::Serine/threonine protein phosphatase 1 (PP1) regulates multiple cellular processes. Protein phosphorylation-dephosphorylation is largely altered during ischemia and subsequent reperfusion. The brain is particularly vulnerable to stress resulting from ischemia-reperfusion (IR), however, the acquisition of ischemic tole...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700214
更新日期:2007-09-01 00:00:00
abstract::We report on a novel strategy for identification of specific sulfation motifs in chondroitin/dermatan sulfate (CS/DS) chain derived from decorin (Dcn), based on enzyme cleavage and multistage MS (MS(n)). Released CS/DS chains were digested with chondroitin B and in parallel with AC I lyases to obtain oligosaccharides ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800440
更新日期:2009-07-01 00:00:00
abstract::Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900289
更新日期:2020-06-01 00:00:00
abstract::Articles assembled in the second part of this Special Issue describe some experimental and computational approaches for the structural and functional characterization of intrinsically disordered proteins. Since these tools represent specialized gear for the focused analysis of various aspects of dark proteome, they ca...
journal_title:Proteomics
pub_type: 社论
doi:10.1002/pmic.201900085
更新日期:2019-03-01 00:00:00
abstract::The plenary session of the Proteomics Standards Initiative of the Human Proteome Organisation at the 6th annual HUPO world congress updated the delegates on the current status of the ongoing work of this group. The new MS interchange format, mzML, had been entered into the HUPO document process immediately prior to th...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200701086
更新日期:2008-04-01 00:00:00