Quantitative Proteomic Analysis of Seminal Plasma, Sperm Membrane Proteins, and Seminal Extracellular Vesicles Suggests Vesicular Mechanisms Aid in the Removal and Addition of Proteins to the Ram Sperm Membrane.

abstract：:As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...

journal_title：Proteomics

authors： Wang W,Guo T,Song T,Lee CS,Balgley BM

更新日期：2007-04-01 00:00:00

abstract：:"Liquid biopsies" have received attention as a complementary tool for traditional tissue biopsies that may enhance the spectrum of analysis for tumor-derived factors. One such factor gaining prominence in the liquid biopsy field is extracellular vesicles (EVs), membrane-bound nanovesicles which are secreted by cells i...

journal_title：Proteomics

authors： Ruhen O,Meehan K

更新日期：2019-01-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:Proteomic analysis on sperm has been restricted to only a few model organisms. We present here a 2DE PAGE proteome map of sperm cells from a nonmodel organism, the marine mussel Mytilus edulis, a free-spawning marine invertebrate with external fertilization. Ninety-six protein spots showing high expression were select...

journal_title：Proteomics

authors： Diz AP,Dudley E,Skibinski DO

更新日期：2012-06-01 00:00:00

abstract：:This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. T...

journal_title：Proteomics

authors： Heo SH,Choi JH,Kim YM,Jung CW,Lee J,Jin HY,Kim GH,Lee BH,Shin CH,Yoo HW

更新日期：2013-04-01 00:00:00

abstract：:MALDI-MS Imaging is a novel label-free technique that can be used to visualize the changes in multiple mass responses following treatment. Following treatment with proinflammatory cytokine interleukin-22 (IL-22), the epidermal differentiation of Labskin, a living skin equivalent (LSE), successfully modeled psoriasis i...

journal_title：Proteomics

authors： Harvey A,Cole LM,Day R,Bartlett M,Warwick J,Bojar R,Smith D,Cross N,Clench MR

更新日期：2016-06-01 00:00:00

abstract：:Plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. Some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergisti...

journal_title：Proteomics

authors： da Silva AJ,Gómez-Mendoza DP,Junqueira M,Domont GB,Ximenes Ferreira Filho E,de Sousa MV,Ricart CA

更新日期：2012-08-01 00:00:00

abstract：:Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...

journal_title：Proteomics

authors： Riwaldt S,Pietsch J,Sickmann A,Bauer J,Braun M,Segerer J,Schwarzwälder A,Aleshcheva G,Corydon TJ,Infanger M,Grimm D

更新日期：2015-09-01 00:00:00

abstract：:Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...

journal_title：Proteomics

authors： Agrawal GK,Rakwal R,Yonekura M,Kubo A,Saji H

更新日期：2002-08-01 00:00:00

abstract：:The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A l...

journal_title：Proteomics

authors： Korf U,Derdak S,Tresch A,Henjes F,Schumacher S,Schmidt C,Hahn B,Lehmann WD,Poustka A,Beissbarth T,Klingmüller U

更新日期：2008-11-01 00:00:00

abstract：:Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...

journal_title：Proteomics

authors： Yu H,Hackenbroch L,Meyer FRL,Reiser J,Razzazi-Fazeli E,Nöbauer K,Besenfelder U,Vogl C,Brem G,Mayrhofer C

更新日期：2019-03-01 00:00:00

abstract：:The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...

journal_title：Proteomics

authors： Rahman MA,Karl K,Nonaka M,Fujimura H,Shinjo R,Oomori T,Wörheide G

更新日期：2014-11-01 00:00:00

abstract：:Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...

journal_title：Proteomics

authors： Kim SY,Joglekar MV,Hardikar AA,Phan TH,Khanal D,Tharkar P,Limantoro C,Johnson J,Kalionis B,Chrzanowski W

更新日期：2019-09-01 00:00:00

abstract：:This study aimed at establishing a sensitive multiple reaction monitoring-mass spectrometry (MRM-MS) method for the quantification of the drug metabolizing cytochrome P450 (CYP)3A4 enzyme in human liver homogenates. Liver samples were subjected to trypsin digestion. MRM-MS analyses were performed using three transitio...

journal_title：Proteomics

authors： Cieślak A,Kelly I,Trottier J,Verreault M,Wunsch E,Milkiewicz P,Poirier G,Droit A,Barbier O

更新日期：2016-11-01 00:00:00

abstract：:The field of biochemistry is currently faced with the enormous challenge of assigning functional significance to more than thirty thousand predicted protein products encoded by the human genome. In order to accomplish this daunting task, methods will be required that facilitate the global analysis of proteins in compl...

journal_title：Proteomics

authors： Patricelli MP,Giang DK,Stamp LM,Burbaum JJ

更新日期：2001-09-01 00:00:00

abstract：:A brief period of ischemia followed by timely reperfusion may lead to prolonged, yet reversible, contractile dysfunction (myocardial stunning). Damage to the myocardium occurs not only during ischemia, but also during reperfusion, where a massive release of oxygen-free radicals (OFR) occurs. We have previously utilize...

journal_title：Proteomics

authors： White MY,Tchen AS,McCarron HC,Hambly BD,Jeremy RW,Cordwell SJ

更新日期：2006-12-01 00:00:00

abstract：:Phosphorylation of proteins is one of the most prominent PTMs and for instance a key regulator of signal transduction. In order to improve our understanding of cellular phosphorylation events, considerable effort has been devoted to improving the analysis of phosphorylation by MS-based proteomics. Different enrichment...

journal_title：Proteomics

authors： Eyrich B,Sickmann A,Zahedi RP

更新日期：2011-02-01 00:00:00

abstract：:Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenes...

journal_title：Proteomics

authors： Ray S,Reddy PJ,Jain R,Gollapalli K,Moiyadi A,Srivastava S

更新日期：2011-06-01 00:00:00

abstract：:Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...

journal_title：Proteomics

authors： ten Have S,Boulon S,Ahmad Y,Lamond AI

更新日期：2011-03-01 00:00:00

abstract：:Plant secretomics is a newly emerging area of the plant proteomics field. It basically describes the global study of secreted proteins into the extracellular space of plant cell or tissue at any given time and under certain conditions through various secretory mechanisms. A combination of biochemical, proteomics and b...

journal_title：Proteomics

authors： Agrawal GK,Jwa NS,Lebrun MH,Job D,Rakwal R

更新日期：2010-02-01 00:00:00

abstract：:The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...

journal_title：Proteomics

authors： Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh J

更新日期：2006-11-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry (MS/MS) were used to determine the multiple acetylation sites in the histone acetyltransferase (HAT): p300-HAT. Partial cleavage of the peptides containing acetylated lysine residues by trypsin provided a set of nested sequences...

journal_title：Proteomics

authors： Wang D,Thompson P,Cole PA,Cotter RJ

更新日期：2005-06-01 00:00:00

abstract：:Carcinoma of the prostate (CaP) is the second leading cause of cancer-related mortality among American men. While high cure rates are associated with localized CaP, no cure exists for advanced recurrent disease. At present there are no known serologic biomarkers specific to this stage of the disease. Several groups ha...

journal_title：Proteomics

authors： Lam YW,Mobley JA,Evans JE,Carmody JF,Ho SM

更新日期：2005-07-01 00:00:00

abstract：:Accumulating evidence suggests that extracellular alpha-synuclein (eSNCA) plays an important role in the pathogenesis of Parkinson's disease or related synucleinopathies by inducing neurotoxicity directly or indirectly via microglial or astroglial activation. However, the mechanisms by which this occurs remain to be c...

journal_title：Proteomics

authors： Liu J,Shi M,Hong Z,Zhang J,Bradner J,Quinn T,Beyer RP,Mcgeer PL,Chen S,Zhang J

更新日期：2010-06-01 00:00:00

abstract：:Toxic oil syndrome (TOS) is a disease that appeared in Spain in 1981. It affected more than 20 000 people and produced over 300 deaths in the first 2 years. In this paper, a prospective study on the differences in gene expression in sera between a control versus a TOS-affected population, both originally exposed to th...

journal_title：Proteomics

authors： Quero C,Colomé N,Prieto MR,Carrascal M,Posada M,Gelpí E,Abian J

更新日期：2004-02-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrP(TSE)) of the host-encoded cellular prion protein (PrP(C)). PrP(TSE) has a central role in the pathogenesis of the disease but other factors are likely involved ...

journal_title：Proteomics

authors： Giorgi A,Di Francesco L,Principe S,Mignogna G,Sennels L,Mancone C,Alonzi T,Sbriccoli M,De Pascalis A,Rappsilber J,Cardone F,Pocchiari M,Maras B,Schininà ME

更新日期：2009-08-01 00:00:00

abstract：:Seed oil bodies (OBs) are intracellular particles storing lipids as food or biofuel reserves in oleaginous plants. Since Brassica napus OBs could be easily contaminated with protein bodies and/or myrosin cells, they must be purified step by step using floatation technique in order to remove non-specifically trapped pr...

journal_title：Proteomics

authors： Jolivet P,Boulard C,Bellamy A,Larré C,Barre M,Rogniaux H,d'Andréa S,Chardot T,Nesi N

更新日期：2009-06-01 00:00:00

abstract：:Human butyrophilin (BTN) expression in milk fat globule (MFGM) was evaluated using two dimensional electrophoresis (2-DE) as the separation technique, and peptide mass mapping by matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) as the identification tool. Since milk composition changes througho...

journal_title：Proteomics

authors： Cavaletto M,Giuffrida MG,Fortunato D,Gardano L,Dellavalle G,Napolitano L,Giunta C,Bertino E,Fabris C,Conti A

更新日期：2002-07-01 00:00:00

abstract：:Two-dimensional electrophoresis is a widely used method for separating a large number of proteins from complex protein mixtures and for revealing differential patterns of protein expressions. In the computer-assisted proteome research, the comparison of protein separation profiles involves several heuristic steps, ran...

journal_title：Proteomics

authors： Salmi J,Aittokallio T,Westerholm J,Griese M,Rosengren A,Nyman TA,Lahesmaa R,Nevalainen O

更新日期：2002-11-01 00:00:00

abstract：:Radiation therapy (RT), combined with chemotherapy, is currently the standard adjuvant approach for UICC-stage II and III rectal cancer patients. Individual rectal tumors display wide ranges of radiosensitivity (RS). The aim of the present study was to identify proteins associated with radioresistance (RR), with the f...

journal_title：Proteomics

authors： Allal AS,Kähne T,Reverdin AK,Lippert H,Schlegel W,Reymond MA

更新日期：2004-08-01 00:00:00