Tumor-Derived Extracellular Vesicles as a Novel Source of Protein Biomarkers for Cancer Diagnosis and Monitoring.

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:Targeted quantitative proteomic analyses aim at systematically measuring the abundance of proteins in large sets of samples, without biases or missing values. One typical implementation is the verification of biomarker candidates in bodily fluids, which measures extended lists of validated transitions using triple qua...

journal_title：Proteomics

authors： Lesur A,Domon B

更新日期：2015-03-01 00:00:00

abstract：:The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...

journal_title：Proteomics

authors： Jung SO,Ro HS,Kho BH,Shin YB,Kim MG,Chung BH

更新日期：2005-11-01 00:00:00

abstract：:Diurnal physiological acclimation regulated by a circadian system is an advantage for plant fitness. The circadian system is composed of a signal input, the clock and output pathways. Understanding the regulation mechanism of the output pathways remains a major challenge. Diurnal proteomic change reflects the state of...

journal_title：Proteomics

authors： Wang Z,Wang T

更新日期：2011-01-01 00:00:00

abstract：:There is ample evidence for the involvement of protein phosphorylation on serine/threonine/tyrosine in bacterial signaling and regulation, but very few exact phosphorylation sites have been experimentally determined. Recently, gel-free high accuracy MS studies reported over 150 phosphorylation sites in two bacterial m...

journal_title：Proteomics

authors： Miller ML,Soufi B,Jers C,Blom N,Macek B,Mijakovic I

更新日期：2009-01-01 00:00:00

abstract：:Scab, caused by Fusarium graminearum, is a serious spike disease in wheat. To identify proteins in resistant wheat cultivar Wangshuibai induced by F. graminearum infection, proteins extracted from spikes 6, 12 and 24 h after inoculation were separated by 2-DE. Thirty protein spots showing 3-fold change in abundance wh...

journal_title：Proteomics

authors： Wang Y,Yang L,Xu H,Li Q,Ma Z,Chu C

更新日期：2005-11-01 00:00:00

abstract：:Basal-like breast cancers are commonly negative for expression of estrogen and progesterone receptors and HER-2 (triple-negative breast cancer), which makes this subtype of breast cancers more aggressive and less responsive to standard treatment. We have applied a small-scale chemical proteomics method using bisindoly...

journal_title：Proteomics

authors： Dolai S,Xu Q,Liu F,Molloy MP

更新日期：2011-07-01 00:00:00

abstract：:Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...

journal_title：Proteomics

authors： Uchiyama N,Kuno A,Tateno H,Kubo Y,Mizuno M,Noguchi M,Hirabayashi J

更新日期：2008-08-01 00:00:00

abstract：:Biotherapeutics, molecules produced from biological systems, require rigorous purification steps to remove impurities including host cell proteins (HCPs). Regulatory guidelines require manufacturers to monitor process-related impurities along the purification workflow. Mass spectrometry (MS) has recently been consider...

journal_title：Proteomics

authors： Trauchessec M,Hesse AM,Kraut A,Berard Y,Herment L,Fortin T,Bruley C,Ferro M,Manin C

更新日期：2021-01-18 00:00:00

abstract：:Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...

journal_title：Proteomics

authors： Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

更新日期：2005-04-01 00:00:00

abstract：:Normal Chinese Liver Proteome Expression Profile is one of the major parts of Human Liver Proteome Project. Before starting the studies, it is necessary to examine the interindividual variation of normal liver proteome and evaluate the minimal size of samples for proteomic analysis. In this study, normal liver samples...

journal_title：Proteomics

authors： Zhang X,Guo Y,Song Y,Sun W,Yu C,Zhao X,Wang H,Jiang H,Li Y,Qian X,Jiang Y,He F

更新日期：2006-10-01 00:00:00

abstract：:The International Plant Proteomics Organization (INPPO) is a global platform of the plant proteomics community or, more generally, the scientific community that uses proteomics to address plant biology. Organizing an international conference is one of its initiatives to promote plant proteomics by involving and gather...

journal_title：Proteomics

authors： Lüthje S,Renaut J,Job D,Hajduch M,Carpentier S,Sarkar A,Agrawal R,Dunn MJ,Rakwal R,Agrawal GK

更新日期：2015-05-01 00:00:00

abstract：:Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...

journal_title：Proteomics

authors： Hu S,Xie Y,Ramachandran P,Ogorzalek Loo RR,Li Y,Loo JA,Wong DT

更新日期：2005-04-01 00:00:00

abstract：:Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high-throughput ...

journal_title：Proteomics

authors： Dam S,Dyrlund TF,Ussatjuk A,Jochimsen B,Nielsen K,Goffard N,Ventosa M,Lorentzen A,Gupta V,Andersen SU,Enghild JJ,Ronson CW,Roepstorff P,Stougaard J

更新日期：2014-02-01 00:00:00

abstract：:Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is still limited. This is because we have so far mostly relied on g...

journal_title：Proteomics

authors： Simicevic J,Deplancke B

更新日期：2017-02-01 00:00:00

abstract：:Cerebrotendinous xanthomatosis (CTX) is an autosomal recessive lipid-storage disorder caused by a deficiency of the mitochondrial sterol 27-hydroxylase. Genetic analysis utilizing SSCP and direct DNA sequencing identified a new mutation. One base-pair of cytosine was deleted at codon 326 on exon 2 of CYP27 in all CTX ...

journal_title：Proteomics

authors： Wang PW,Chang WN,Lu CH,Chao D,Schrag C,Pan TL

更新日期：2006-02-01 00:00:00

abstract：:With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...

journal_title：Proteomics

authors： Bereman MS

更新日期：2015-03-01 00:00:00

abstract：:Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...

journal_title：Proteomics

authors： Echan LA,Tang HY,Ali-Khan N,Lee K,Speicher DW

更新日期：2005-08-01 00:00:00

abstract：:Brain development and aging is a complex process involving proliferation, differentiation and apoptosis. Elucidating proteome changes in these processes can help to understand the mechanisms of brain development and maintenance as well as neurodegenerative diseases. The research reported here is a contribution to the ...

journal_title：Proteomics

authors： Föcking M,Boersema PJ,O'Donoghue N,Lubec G,Pennington SR,Cotter DR,Dunn MJ

更新日期：2006-09-01 00:00:00

abstract：:The availability of completed genome sequences of several eukaryotic and prokaryotic species has shifted the focus towards the identification and characterization of all gene products that are expressed in a given organism. In order to cope with the huge amounts of data that have been provided by large-scale sequencin...

journal_title：Proteomics

authors： Auerbach D,Thaminy S,Hottiger MO,Stagljar I

更新日期：2002-06-01 00:00:00

abstract：:We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...

journal_title：Proteomics

authors： Ro HS,Koh BH,Jung SO,Park HK,Shin YB,Kim MG,Chung BH

更新日期：2006-04-01 00:00:00

abstract：:Phosphoproteomics can be defined as the comprehensive study of protein phosphorylation by identification of the phosphoproteins, exact mapping of the phosphorylation sites, quantification of phosphorylation, and eventually, revealing their biological function. Its place in today's research is vitally important to addr...

journal_title：Proteomics

authors： Kersten B,Agrawal GK,Iwahashi H,Rakwal R

更新日期：2006-10-01 00:00:00

abstract：:Protein quantification at proteome-wide scale is an important aim, enabling insights into fundamental cellular biology and serving to constrain experiments and theoretical models. While proteome-wide quantification is not yet fully routine, many datasets approaching proteome-wide coverage are becoming available throug...

journal_title：Proteomics

authors： Wang M,Herrmann CJ,Simonovic M,Szklarczyk D,von Mering C

更新日期：2015-09-01 00:00:00

abstract：:The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...

journal_title：Proteomics

authors： Odom GJ,Ban Y,Colaprico A,Liu L,Silva TC,Sun X,Pico AR,Zhang B,Wang L,Chen X

更新日期：2020-11-01 00:00:00

abstract：:Sustainable innovations in sequencing technologies have resulted in a torrent of microbial genome sequencing projects. However, the prokaryotic genomes sequenced so far are unequally distributed along their phylogenetic tree; few phyla contain the majority, the rest only a few representatives. Accurate genome annotati...

journal_title：Proteomics

authors： Kumar D,Mondal AK,Kutum R,Dash D

更新日期：2016-01-01 00:00:00

abstract：:In a comparative study, we investigated the influence of nine sample preparation workflows and seven different lysis buffers for qualitative and quantitative analysis of the human adipose tissue proteome. Adipose tissue is not just a fat depot but also an endocrine organ, which cross-talks with other tissue types and ...

journal_title：Proteomics

authors： Pasing Y,Colnoe S,Hansen T

更新日期：2017-03-01 00:00:00

abstract：:Low root temperature causes a decrease in water uptake, which leads to mineral and nutrient deficiencies with potentially decreased root and shoot growth. Differential temperature effects in plants have been studied extensively, however, the effect of root chilling on the global protein expression in shoots has not be...

journal_title：Proteomics

authors： Neilson KA,Scafaro AP,Chick JM,George IS,Van Sluyter SC,Gygi SP,Atwell BJ,Haynes PA

更新日期：2013-06-01 00:00:00

abstract：:The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...

journal_title：Proteomics

authors： González-Camacho F,Medina FJ

更新日期：2004-02-01 00:00:00

abstract：:The 2-D peptide separations employing mixed mode reversed phase anion exchange (MM (RP-AX)) HPLC in the first dimension in conjunction with RP chromatography in the second dimension were developed and utilised for shotgun proteome analysis. Compared with strong cation exchange (SCX) typically employed for shotgun prot...

journal_title：Proteomics

authors： Phillips HL,Williamson JC,van Elburg KA,Snijders AP,Wright PC,Dickman MJ

更新日期：2010-08-01 00:00:00

abstract：:Cofilin, an actin-binding protein, regulates the rate, nature and extent of assembly of the actin cytoskeleton. Native Phast gels show that the addition of cofilin to an actin-DNase I complex (74 kDa) results in the formation of a ternary complex of 94 kDa indicating an equimolar stoichiometry in the ternary complex. ...

journal_title：Proteomics

authors： Nosworthy NJ,Kekic M,dos Remedios CG

更新日期：2001-12-01 00:00:00